Monkey IL-6 ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey IL-6 with a sensitivity of 3.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Frontiers in aging neuroscience 14:9186402022The Effect of Sevoflurane Anesthesia on the Biomarkers of Neural Injury in the Prefrontal Cortex of Aged Marmosets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanyong Cheng,Lingling Shi,Haoli Mao,Zhenyu Xue,Siyu Liu,Zilong Qiu,Lei Zhang,Hong JiangPubMed 35847680
- Cell 185:1728-1744.e162022Circular RNA vaccines against SARS-CoV-2 and emerging variants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Qu,Zongyi Yi,Yong Shen,Liangru Lin,Feng Chen,Yiyuan Xu,Zeguang Wu,Huixian Tang,Xiaoxue Zhang,Feng Tian,Chunhui Wang,Xia Xiao,Xiaojing Dong,Li Guo,Shuaiyao Lu,Chengyun Yang,Cong Tang,Yun Yang,Wenhai Yu,Junbin Wang,Yanan Zhou,Qing Huang,Ayijiang Yisimayi,Shuo Liu,Weijin Huang,Yunlong Cao,Youchun Wang,Zhuo Zhou,Xiaozhong Peng,Jianwei Wang,Xiaoliang Sunney Xie,Wensheng WeiPubMed 35460644
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Monkey, Cynomolgus monkey, Rhesus monkey
- Range
- 12.5 - 800 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 3.2 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells. IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury (By similarity). In the adaptive immune response, is required for the differentiation of B cells into immunoglobulin-secreting cells. Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Required to drive naive CD4(+) T cells to the Th17 lineage. Also required for proliferation of myeloma cells and the survival of plasmablast cells (By similarity). Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability. Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF. Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance (By similarity). 'Trans-signaling' in central nervous system also regulates energy and glucose homeostasis. Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand (By similarity). Also acts as a myokine (By similarity). Plays a protective role during liver injury, being required for maintenance of tissue regeneration (By similarity). Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection (By similarity). Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration (By similarity).
Alternative names
Interleukin-6, IL-6, IL6
What's included?
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Monkey IL-6 ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey IL-6 with a sensitivity of 3.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Monkey, Cynomolgus monkey, Rhesus monkey
- Range
- 12.5 - 800 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 3.2 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Cell Media | n 8 | C.V. 5.5 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Cell Media | n 3 | C.V. 5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 90 | Range 86 - 94 % |
Sample type Serum | Average % = 76 | Range 75 - 77 % |
Sample type EDTA Plasma | Average % = 85 | Range 78 - 92 % |
Sample type Heparin Plasma | Average % = 103 | Range 94 - 114 % |
Sample type Citrate plasma | Average % = 78 | Range 76 - 81 % |
Sample type Cell culture media | Average % = 101 | Range 95 - 109 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Monkey IL-6 ELISA Kit ab242233 is a rapid single-wash 90-min Sandwich ELISA to measure Monkey IL-6 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 3.2 pg/mL.
How the assay works
Monkey IL-6 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Monkey IL-6 ELISA Kit ab242233 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Save your precious samples
Our 384-well Monkey IL-6 ELISA Kit only requires a maximum of 12.5µL sample
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Interleukin-6 (IL-6) a cytokine also known as IFN-beta2 plays a significant role in immune response and inflammation. The IL-6 protein has a molecular weight of approximately 20-26 kDa. Expression of IL-6 occurs in various cell types including T cells macrophages and fibroblasts. Researchers often measure IL-6 levels in biological samples using IL-6 ELISA kits an essential tool for studying this protein’s function and presence in experimental and clinical settings.
- Biological function summary
IL-6 influences immune regulation and acts as part of the acute phase response. It stimulates the production of acute-phase proteins and supports the differentiation of B cells into antibody-producing cells. IL-6 is not known to be part of a larger complex acting primarily as a single entity in signal transduction. Moreover IL-6 impacts the metabolism of iron and bone homeostasis showing its multifunctional nature.
- Pathways
IL-6 forms an integral part of several signaling routes particularly the JAK-STAT pathway. In this context IL-6 interacts with signal transducer proteins like STAT3 to transmit signals from the cell surface to the nucleus affecting gene expression. Another important pathway is the MAPK pathway through which IL-6 influences cell proliferation and survival. These interactions reflect IL-6's diverse effects in cellular processes.
- Associated diseases and disorders
IL-6's association with rheumatoid arthritis and multiple myeloma emphasizes its role in chronic inflammation and cancer. In rheumatoid arthritis IL-6 contributes to inflammation and joint damage often together with TNF-alpha highlighting a potential target for anti-inflammatory therapies. In multiple myeloma IL-6 supports the survival and proliferation of cancerous plasma cells highlighting its importance in cancer progression and possible treatment targets.
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5 product images
ELISA - Monkey IL-6 ELISA Kit (ab242233)
Sandwich ELISA - Monkey IL-6 ELISA Kit (ab242233)
Example of monkey IL-6 standard curve in Sample Diluent NS. The IL-6 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Monkey IL-6 ELISA Kit (ab242233)
Interpolated concentrations of native IL-6 in Rhesus monkey Peripheral Blood Monocyte (PBMC) cell culture supernatant samples.
PBMCs were cultured in the presence (stimulated) or absence of 50 ng/mL PMA + 1 μg/mL Ionomycin for 24 hours. The concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 concentration was determined to be 696.3 pg/mL in neat stimulated PBMC cell culture supernatant, and undetectable in unstimulated PBMC cell culture supernatant, and naive cell culture media controls (not shown).
Sandwich ELISA - Monkey IL-6 ELISA Kit (ab242233)
Comparison of native IL-6 in rhesus and cynomolgus stimulated peripheral blood mononuclear cell (PBMC) supernatant samples.
PBMCs were cultured in the presence or absence of 50 ng/mL PMA and 1 μg/mL Ionomycin for 24 hours. The concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 concentration was determined to be 696.3 pg/mL in neat stimulated rhesus PBMC supernatant and 907.3 pg/mL in neat stimulated cynomolgus PBMC supernatant. Values for unstimulated supernatants were undetectable in both rhesus and cynomolgus samples (not shown).
Sandwich ELISA - Monkey IL-6 ELISA Kit (ab242233)
Example of monkey IL-6 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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