Monkey IL-8 ELISA Kit
- Recombinant
- SimpleStep
- What is this?
Be the first to review this product! Submit a review
|
(0 Publication)
Monkey IL-8 ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey IL-8 with a sensitivity of 2.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8
- ELISA
Supplier Data
ELISA - Monkey IL-8 ELISA Kit (AB242232)
- sELISA
Unknown
Sandwich ELISA - Monkey IL-8 ELISA Kit (AB242232)
Example of monkey IL-8 standard curve in Sample Diluent NS. The IL-8 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Unknown
Sandwich ELISA - Monkey IL-8 ELISA Kit (AB242232)
Interpolated concentrations of native IL-8 in stimulated and unstimulated Rhesus macaque peripheral blood mononuclear cell (PBMC) supernatant samples.
Rhesus macaque PBMCs were cultured in the presence (stimulated) or absence (unstimulated) of 50 ng/mL PMA and 1 μg/mL ionomycin for 24 hours. The concentrations of IL-8 were measured in duplicates, interpolated from the IL-8 standard curves and corrected for sample dilution. Undiluted samples are as follows : stimulated PBMC supernatant 3%, and unstimulated PBMC supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-8 concentration was determined to be 9,926 pg/mL in neat stimulated PBMC cell culture supernatant and 36.36 pg/mL in neat unstimulated PBMC cell culture supernatant. IL-6 was undetectable in naive cell culture media (not shown).
- sELISA
Unknown
Sandwich ELISA - Monkey IL-8 ELISA Kit (AB242232)
Comparison of rhesus macaque and cynomolgus monkey serum, plasma, and PMA + Ionomycin stimulated and unstimulated PBMC cell culture supernatants (SN) (24 hours). The concentrations of IL-8 were measured in duplicates, interpolated from the IL-8 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values in neat samples are plotted (mean +/- SD, n=2) and also shown in the table below.
- sELISA
Unknown
Sandwich ELISA - Monkey IL-8 ELISA Kit (AB242232)
Interpolated concentrations of native IL-8 in monkey serum and plasma samples. The concentrations of IL-8 were measured in duplicates, interpolated from the IL-8 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10%, plasma (heparin) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-8 concentration was determined to be 2,072 pg/mL in neat serum, 644.2 pg/mL in neat plasma (heparin), and 248.7 pg/mL in neat plasma (EDTA).
Complementary Products
Reactivity data
Product details
How the assay works
Monkey IL-8 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Monkey IL-8 ELISA Kit ab242232 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
IL-8 functions as a chemoattractant for neutrophils and lymphocytes facilitating their movement towards the site of infection or injury. It does not form part of a larger protein complex but operates individually to enhance immune cell migration. IL-8 possesses unique binding motifs allowing it to interact with specific receptors namely CXCR1 and CXCR2 on target cells. This binding triggers cellular responses leading to effective immune surveillance and response to inflammatory stimuli.
Pathways
IL-8 operates within important inflammatory and immune response pathways. It forms a part of the NF-κB signaling cascade which activates in response to stress signals promoting the expression of other inflammatory mediators. Additionally it engages in the mitogen-activated protein kinase (MAPK) pathway influencing cellular responses such as proliferation and differentiation. The interaction of IL-8 with these pathways highlights its role in modulating immune responses and highlights its interaction with other proteins such as TNF-α and IL-1β.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com