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Monkey Interferon gamma ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Monkey Interferon gamma in Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cerebral Spinal Fluid, Cell culture supernatant samples.

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Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cerebral Spinal Fluid, Cell culture supernatant

Assay type

Sandwich (quantitative)

Reactive species

Rhesus monkey

Range

93.75 - 6000 pg/mL

Assay time

1h 30m

Sensitivity

= 25.374 pg/mL

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Shipping To:
United States

Images

Target data

What's included?

1 x 96 Tests
Components
10X Monkey Interferon gamma Capture Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent 4BI
1 x 6 mL
Monkey Interferon gamma Detector Antibody
1 x 600 µL
Monkey Interferon gamma Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL

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Monkey Interferon gamma ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Monkey Interferon gamma in Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cerebral Spinal Fluid, Cell culture supernatant samples.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cerebral Spinal Fluid, Cell culture supernatant

Assay type

Sandwich (quantitative)

Reactive species

Rhesus monkey

Range

93.75 - 6000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 25.374 pg/mL

General recovery

94%

Precision

Intra assay

Sample
Supernatant
n
8
mean
-
SD
-
C.V.
2.9

Inter assay

Sample
Supernatant
n
3
mean
-
SD
-
C.V.
2.2

Recovery

Sample specific recovery

Sample type
Serum
Average %
= 94
Range
91 - 98 %
Sample type
EDTA Plasma
Average %
= 99
Range
95 - 106 %
Sample type
Citrate plasma
Average %
= 101
Range
96 - 109 %
Sample type
Heparin Plasma
Average %
= 110
Range
109 - 112 %
Sample type
Cerebral Spinal Fluid
Average %
= 105
Range
100 - 110 %
Sample type
Cell culture supernatant
Average %
= 110
Range
108 - 115 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Monkey Interferon gamma SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Interferon gamma protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, Cerebral Spinal Fluid, Cell culture supernatant. Quantitate Monkey Interferon gamma with 25.374 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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