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AB326898

Monkey Interferon gamma ELISA Kit, Chemiluminescent

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Monkey Interferon gamma ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Monkey Interferon gamma with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 10.97 - 24000 pg/ml with a sensitivity of 2.47 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

Interferon gamma, IFN-gamma, IFNG

3 Images
Sandwich ELISA - Monkey Interferon gamma ELISA Kit, Chemiluminescent (AB326898)
  • sELISA

Lab

Sandwich ELISA - Monkey Interferon gamma ELISA Kit, Chemiluminescent (AB326898)

Dynamic range comparison between the Chemiluminescent Monkey Interferon gamma SimpleStep ELISA and the colorimetric Monkey Interferon gamma SimpleStep ELISA (ab315428). The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range

Sandwich ELISA - Monkey Interferon gamma ELISA Kit, Chemiluminescent (AB326898)
  • sELISA

Lab

Sandwich ELISA - Monkey Interferon gamma ELISA Kit, Chemiluminescent (AB326898)

Dynamic range comparison between the Chemiluminescent Monkey Interferon gamma SimpleStep ELISA and the colorimetric Monkey Interferon gamma SimpleStep ELISA (ab315428). The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Monkey Interferon gamma ELISA Kit, Chemiluminescent (AB326898)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey Interferon gamma ELISA Kit, Chemiluminescent (AB326898)

Interpolated concentrations of native Interferon gamma in cynomolgus monkey unstimulated and stimulated PBMC cell culture supernatant samples

Interpolated concentration of native Interferon gamma was measured in duplicate at different sample concentrations. Undiluted samples are as follows : unstimulated PBMC cell culture supernatant (50%) and stimulated PBMC cell culture supernatant (2.5%) treated with PMA (50 ng/mL) and ionomycin (1 µg/mL) for 24 hrs.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Luminescent

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cerebral Spinal Fluid, Cell culture supernatant

Reacts with

Human, Monkey

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.47 pg/mL

Range

10.97 - 24000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Monkey Interferon gamma ELISA Kit, Chemiluminescent (ab326898) is a rapid single-wash sandwich 55-min ELISA to measure Monkey Interferon gamma in Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cerebral Spinal Fluid, Cell culture supernatant with a sensitivity of 2.47 pg/mL.

How the assay works

Monkey Interferon gamma ELISA Kit, Chemiluminescent (ab326898) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Monkey Interferon gamma (ab326898) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Interferon gamma antibody [EPR24641-55] - BSA and Aside free (Capture) ab289984
- Anti-Interferon gamma antibody [EPR24641-20] - BSA and Aside free (Detector) ab289983
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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.9" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.2" } ]
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Recovery

[ { "sample": "Serum", "range": "91 - 98 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "95 - 106 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "96 - 109 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "109 - 112 %", "average": "= 110" }, { "sample": "Cerebral Spinal Fluid", "range": "100 - 110 %", "average": "= 105" }, { "sample": "Cell culture supernatant", "range": "108 - 115 %", "average": "= 110" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (By similarity). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).
See full target information IFNG
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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