Monkey IP-10 (CXCL10) ELISA Kit
- SimpleStep
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Monkey IP-10 (CXCL10) ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey IP-10 (CXCL10) with a sensitivity of 1.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
View Alternative Names
SCYB10, CXCL10, C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, Small-inducible cytokine B10, Gamma-IP10, IP-10
- sELISA
Supplier Data
Sandwich ELISA - Monkey IP-10 (CXCL10) ELISA Kit (AB314607)
Representative image of Monkey IP-10 (CXCL10) ELISA Kit (ab314607)
Components shown from left to right :
- Sample diluent NS
- 10X Monkey IP-10 (CXCL10) Capture Antibody
- 10X Monkey IP-10 (CXCL10) Detector Antibody
- Monkey IP-10 (CXCL10) Lyophilised Purified Protein (2 vials)
- 50X Cell Extraction Enhancer Solution (ab193971)
- Stop Solution
- TMB solution
- 10X Wash Buffer PT (ab206977)
- Antibody Diluent 4BI
SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.
Plate Seals - shown underneath bottles / vials.
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.
- sELISA
Supplier Data
Sandwich ELISA - Monkey IP-10 (CXCL10) ELISA Kit (AB314607)
Interpolated concentration of native IP-10 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : plasma (EDTA) 50% and cerebrospinal fluid 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + Enhancer.
- sELISA
Supplier Data
Sandwich ELISA - Monkey IP-10 (CXCL10) ELISA Kit (AB314607)
Example of rhesus macaque IP-10 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Monkey IP-10 (CXCL10) ELISA Kit (AB314607)
Interpolated concentration of native IP-10 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : untreated PBMC supernatant 100% and treated PBMC supernatant 5%. PMBC’s were cultured in the presence and absence of 50 ng/mL PMA and 1 µg/mL ionomycin for 24 hours. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Monkey IP-10 (CXCL10) ELISA Kit (AB314607)
Example of rhesus macaque IP-10 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Monkey IP-10 (CXCL10) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IP-10 (CXCL10) protein in EDTA Plasma, Cerebral Spinal Fluid, Cell culture supernatant. Quantitate Monkey IP-10 (CXCL10) with 1.3 pg/ml sensitivity. SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
IP10 plays a role in modulating the activities of immune cells. It attracts T cells eosinophils monocytes and natural killer (NK) cells by binding to the CXCR3 receptor. IP10 is not part of a larger complex but interacts with other cytokines to influence cell migration and the immune response. High levels of IP10 can reflect strong immune activation which is why it is often measured in inflammatory conditions using standard assays like the IP-10 ELISA kits.
Pathways
The role of IP10 lies within the Th1-type immune response pathway. In this pathway IP10 works alongside other chemokines to recruit and activate immune cells to sites of inflammation or infection. It synergizes with IFN-γ to propagate immune signals. IP10 is also linked with the CXCR3 receptor which plays a critical role in these pathways providing a connection to other proteins such as CXCL9 and CXCL11 which have similar functions in cell-mediated immunity.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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