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AB283887

Monkey Lipocalin-2 ELISA Kit

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(2 Publications)

Monkey Lipocalin-2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey Lipocalin-2 with a sensitivity of 618 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
4 Images
Sandwich ELISA - Monkey Lipocalin-2 ELISA Kit (AB283887)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey Lipocalin-2 ELISA Kit (AB283887)

Representative image of Monkey Lipocalin-2 ELISA Kit (ab283887)

Components shown from left to right :

- Sample diluent NS

- 10X Monkey Lipocalin-2 Capture Antibody

- 10X Monkey Lipocalin-2 Detector Antibody

- Monkey Lipocalin-2 Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent CPR2

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Sandwich ELISA - Monkey Lipocalin-2 ELISA Kit (AB283887)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey Lipocalin-2 ELISA Kit (AB283887)

Interpolated concentrations of native Lipocalin-2 in monkey serum, plasma samples

Sandwich ELISA - Monkey Lipocalin-2 ELISA Kit (AB283887)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey Lipocalin-2 ELISA Kit (AB283887)

Example of monkey Lipocalin-2 standard curve in Sample Diluent NS +1X Enhancer.

The Lipocalin-2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Monkey Lipocalin-2 ELISA Kit (AB283887)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey Lipocalin-2 ELISA Kit (AB283887)

Interpolated concentrations of native Lipocalin-2 in monkey cell culture supernatant samples.

The concentrations of Lipocalin-2 were measured in duplicates, interpolated from the Lipocalin-2 standard curves and corrected for sample dilution. Undiluted samples are as follows : PBMC treated and untreated supernatant neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipocalin-2 concentration was determined to be 8.29 ng/mL in PBMC treated supernatant. No detectable signal in PBMC untreated supernatant.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Monkey

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 618 pg/mL

Range

937.5 - 60000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Monkey Lipocalin-2 ELISA Kit (ab283887) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Lipocalin-2 protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey Lipocalin-2 with 618 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Lipocalin-2 (also known as Neutrophil gelatinase-associated lipocalin or NGAL) is an iron binding and iron trafficking protein. Lipocalin-2 is involved in multiple cellular processes including apoptosis, innate immunity, and renal development. Deficiency in Lipocalin-2 leads to increased susceptibility to bacterial infection indicating the bacteriostatic function may be related to Lipocalin-2 limiting bacterial iron supply. Studies have shown elevated Lipocalin-2 secretion in the blood and urine after kidney injury in which its levels can be proportionally associated with the severity of prognosis, thus making Lipocalin-2 a useful biomarker for acute kidney injury.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "85 - 90 %", "average": "= 87" }, { "sample": "Serum", "range": "83 - 94 %", "average": "= 90" }, { "sample": "EDTA Plasma", "range": "84 - 90 %", "average": "= 88" }, { "sample": "Heparin Plasma", "range": "82 - 96 %", "average": "= 88" }, { "sample": "Citrate plasma", "range": "82 - 104 %", "average": "= 96" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283887-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB283887-CMP13", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB283887-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB283887-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey Lipocalin-2 Capture Antibody", "number":"AB283887-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Monkey Lipocalin-2 Lyophilized Recombinant Protein", "number":"AB283887-CMP02", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB283887-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB283887-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB283887-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey Lipocalin-2 Detector Antibody", "number":"AB283887-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB283887-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB283887-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information Lipocalin-2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Epigenetics & chromatin 13:6 PubMed32070414

2020

JMJD1B, a novel player in histone H3 and H4 processing to ensure genome stability.

Applications

Unspecified application

Species

Unspecified reactive species

Francisco Saavedra,Zachary A Gurard-Levin,Camila Rojas-Villalobos,Isabelle Vassias,Raquel Quatrini,Geneviève Almouzni,Alejandra Loyola

The Journal of biological chemistry 286:17714-21 PubMed21454524

2011

Sequential establishment of marks on soluble histones H3 and H4.

Applications

Unspecified application

Species

Unspecified reactive species

Francisca Alvarez,Francisca Muñoz,Pierre Schilcher,Axel Imhof,Geneviève Almouzni,Alejandra Loyola
View all publications
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Product promise

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