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AB289698

Monkey MIP-1 alpha/CCL3 ELISA Kit

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Monkey MIP-1 alpha/CCL3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey MIP-1 alpha/CCL3 with a sensitivity of 8.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

C C motif chemokine 3, CCL3_HUMAN, Chemokine (C C motif) ligand 3, Chemokine C C motif ligand 3, Chemokine ligand 3, G0/G1 switch regulatory protein 19-1, G0S19 1, G0S19 1 protein, Heparin binding chemotaxis protein, L2G25B, LD78 alpha, LD78-alpha(4-69), MIP 1A, MIP-1-alpha, MIP-1-alpha(4-69), Macrophage inflammatory protein 1-alpha, PAT 464.1, SCYA 3, SIS alpha, SIS-beta, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein, macrophage inflammatory protein 1a, small inducible cytokine A3 (homologous to mouse Mip-1a)

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Sandwich ELISA - Monkey MIP-1 alpha/CCL3 ELISA Kit (AB289698)
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Supplier Data

Sandwich ELISA - Monkey MIP-1 alpha/CCL3 ELISA Kit (AB289698)

Example of monkey MIP-1 alpha standard curve in Sample Diluent NS.

Example of monkey MIP-1 alpha standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Monkey MIP-1 alpha/CCL3 ELISA Kit (AB289698)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey MIP-1 alpha/CCL3 ELISA Kit (AB289698)

Interpolated concentrations of monkey MIP-1 alpha in PBMC stimulated cell culture supernatant.

Interpolated concentration of native MIP-1 alpha was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 200 PBMC stimulated cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. No detectable signal was observed in unstimulated.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Monkey

Assay type

Sandwich (quantitative)

Sensitivity

= 8.7 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Monkey MIP-1 aplha/CCL3) ELISA Kit (ab289698) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Monkey MIP-1 alpha/CCL3 in serum, plasma (EDTA), plasma (citrate), and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey MIP-1 alpha/CCL3 with 8.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.3" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "97 - 103 %", "average": "= 101" }, { "sample": "Serum", "range": "78 - 79 %", "average": "= 78" }, { "sample": "EDTA Plasma", "range": "97 - 108 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "80 - 85 %", "average": "= 82" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MIP-1 alpha also known as CCL3 is a chemokine that plays a critical role in the immune system. With a molecular weight of around 8 kDa it is primarily expressed in activated T-cells macrophages and fibroblasts. MIP-1 alpha acts as a signaling protein that binds to receptors on target cells directing the movement of immune cells. Researchers commonly refer to it using various alternative names such as MIP-1a and CCL3-alpha rat and it is frequently measured using assays like MIP-1 alpha ELISA for experimental analyses.
Biological function summary

Proteins including MIP-1 alpha participate in the recruitment and activation of diverse immune cells aiding the inflammatory response. Through interaction with receptors like CCR1 and CCR5 MIP-1 alpha mobilizes cells like natural killer cells and monocytes. This chemokine does not generally form part of a larger protein complex but interacts directly with membrane receptors to exert its function in immune cell chemotaxis.

Pathways

MIP-1 alpha operates in the context of both the inflammatory response and the chemokine signaling pathways. Its involvement in these pathways helps mediate the trafficking of leukocytes. Notably MIP-1 alpha interacts with proteins such as CCR1 and CCR5 to transmit signals that instigate immune responses. These proteins are integral to its role in modulating immunity providing targets for research into controlling inflammatory processes.

MIP-1 alpha closely relates to conditions such as rheumatoid arthritis and HIV infection. In rheumatoid arthritis its overexpression can lead to excessive inflammation and joint destruction. Meanwhile in HIV infection MIP-1 alpha interacts with CCR5 a co-receptor necessary for the virus entry into cells. This dual relationship elucidates its potential as a therapeutic target in managing both inflammatory diseases and viral entry mechanisms.
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Product protocols

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Target data

See full target information C-C motif chemokine 3
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websiteProtocolBooklet
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