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AB315314

Monkey MMP3 ELISA Kit

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Monkey MMP3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey MMP3 with a sensitivity of 10.45 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
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Sandwich ELISA - Monkey MMP3 ELISA Kit (AB315314)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey MMP3 ELISA Kit (AB315314)

Interpolated concentration of native MMP3 was measured in duplicate. Undiluted samples are 50% diluted stimulated and unstimulated PBMC cell culture supernatant. Stimulated PBMC cell culture supernatants were treated with 50 ng/mL PMA and 1 µg/mL Ionomycin for 24 hours. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Monkey MMP3 ELISA Kit (AB315314)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey MMP3 ELISA Kit (AB315314)

Interpolated concentration of nativeMMP3 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 5% serum, 3.125% plasma (citrate), 3.125% plasma (EDTA), and 6.25% plasma (heparin).  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Monkey MMP3 ELISA Kit (AB315314)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey MMP3 ELISA Kit (AB315314)

Example of Monkey MMP3 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cerebral Spinal Fluid, Cell culture supernatant

Reacts with

Rhesus monkey

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 10.45 pg/mL

Range

21.875 - 1400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Monkey MMP3 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MMP3 protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, Cerebral Spinal Fluid, and Cell culture supernatant. Quantitate Monkey MMP3 with 10.45 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab315065) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7.9" } ]
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Recovery

[ { "sample": "Serum", "range": "97 - 107 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "95 - 103 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "107 - 110 %", "average": "= 108" }, { "sample": "Heparin Plasma", "range": "91 - 101 %", "average": "= 95" }, { "sample": "Cerebral Spinal Fluid", "range": "97 - 101 %", "average": "= 99" }, { "sample": "Cell culture supernatant", "range": "97 - 105 %", "average": "= 102" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab315314-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB315314-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB315314-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB315314-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Monkey MMP3 Lyophilized Recombinant Protein", "number":"AB315314-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB315314-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315314-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey MMP3 Capture Antibody", "number":"AB315314-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey MMP3 Detector Antibody", "number":"AB315314-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB315314-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB315314-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMP3 also known as stromelysin-1 is a member of the matrix metalloproteinase (MMP) family. These proteins are zinc-dependent endopeptidases. MMP3 specifically has a molecular weight of approximately 54 kDa and is responsible for degrading extracellular matrix components such as collagen fibronectin and laminin. MMP3 is expressed in various tissues including fibroblasts chondrocytes and endothelial cells. It plays a significant role in tissue remodeling wound healing and potentially aids in the breakdown of cellular matrices.
Biological function summary

MMP3 contributes to multiple physiological and pathological processes. It supports the remodeling of connective tissues and assists in normal developmental processes. MMP3 can work as part of a complex with other MMP proteins which can enhance or inhibit its activity. The balance of MMPs including MMP3 regulates the extracellular matrix turnover and is important for maintaining tissue homeostasis.

Pathways

MMP3 participates in pathways involved in tissue remodeling and inflammatory responses. It is part of cellular pathways regulated by cytokines like interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha). These pathways link MMP3 to other proteins such as MMP1 and MMP9 which perform overlapping and distinct roles in matrix degradation during inflammation and tissue repair.

MMP3 is implicated in conditions like arthritis and cardiovascular diseases. MMP3 contributes to cartilage degradation in rheumatoid arthritis and is associated with the breakdown of vascular structures in atherosclerosis. Proteins like MMP9 and MMP13 often interact with MMP3 in these pathological conditions forming a network that exacerbates tissue damage and disease progression.
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Product protocols

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Target data

See full target information MMP3
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websiteProtocolBooklet
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Product promise

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