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AB315295

Monkey Osteopontin ELISA Kit

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Monkey Osteopontin ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey Osteopontin with a sensitivity of 41.516 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
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Sandwich ELISA - Monkey Osteopontin ELISA Kit (AB315295)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey Osteopontin ELISA Kit (AB315295)

Interpolated concentration of native Osteopontin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1.25%, plasma (EDTA) 10%, plasma (citrate) 2.5%, plasma (heparin) 2.5%, and CSF 2.5%.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Monkey Osteopontin ELISA Kit (AB315295)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey Osteopontin ELISA Kit (AB315295)

Interpolated concentration of native Osteopontin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : unstimulated PBMC cell culture supernatant 50% and stimulated PBMC cell culture supernatant 12.5%.  Stimulated PBMC cell culture supernatants were treated with 50 ng/mL PMA and 1 µg/mL Ionomycin for 24 hours. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Monkey Osteopontin ELISA Kit (AB315295)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey Osteopontin ELISA Kit (AB315295)

Example of monkey Osteopontin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cerebral Spinal Fluid, Cell culture supernatant

Reacts with

Rhesus monkey

Assay type

Sandwich (quantitative)

Sensitivity

= 41.516 pg/mL

Range

109.375 - 7000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Monkey Osteopontin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Osteopontin protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Monkey Osteopontin with 41.516 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab315065) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.7" } ]
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Recovery

[ { "sample": "Serum", "range": "90 - 98 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "108 - 121 %", "average": "= 115" }, { "sample": "Citrate plasma", "range": "98 - 111 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "107 - 122 %", "average": "= 115" }, { "sample": "Cerebral Spinal Fluid", "range": "110 - 123 %", "average": "= 115" }, { "sample": "Cell culture supernatant", "range": "102 - 113 %", "average": "= 107" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Osteopontin also known as OPN or secreted phosphoprotein 1 (SPP1) is a glycoprotein with a molecular weight of approximately 44 to 75 kDa depending on its post-translational modifications. Osteopontin is characteristically expressed in bone but is also present in various body tissues including kidney liver and immune system cells. This multifunctional protein interacts with cell surface receptors such as integrins and CD44 playing an important role in cell signaling and extracellular matrix interactions. Researchers often study it using osteopontin western blot or osteopontin ELISA kit to understand its expression patterns and roles.
Biological function summary

Osteopontin affects cell adhesion migration and survival. It does not form part of a known large complex but interacts closely with its integrin receptors. Osteopontin modulates immune responses and aids in bone remodeling by affecting osteoclast and osteoblast functions. It is also influential in inflammation processes where it orchestrates the recruitment and activation of macrophages. The osteopontin function is significant in both physiological processes and pathologies marking it as an important protein to study in numerous biological contexts.

Pathways

Osteopontin is integral to both the bone remodeling and immune response pathways. It collaborates with proteins like osteocalcin and matrix metalloproteinases within these processes. In the bone remodeling pathway osteopontin mediates communication between osteoclasts and osteoblasts impacting bone resorption and formation. In the immune response pathway it coordinates with cytokines such as interleukin-6 to modulate immune cell activities. Its roles in these pathways indicate its multifunctional nature and its impact across different biological systems.

Osteopontin links to cancer and cardiovascular diseases. It facilitates tumor progression and metastasis by interacting with proteins such as vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9). In cardiovascular diseases osteopontin influences atherosclerosis development through interactions with inflammatory cytokines and contributes to plaque stability. These disease associations highlight the importance of osteopontin as both a therapeutic target and a biomarker for disease progression and prognosis.
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Product protocols

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Target data

See full target information Osteopontin
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websiteProtocolBooklet
en
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