AB323522

Monkey RANTES (CCL5) ELISA Kit

SimpleStep
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Monkey RANTES (CCL5) ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey RANTES (CCL5) with a sensitivity of 13.423 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

C-C motif chemokine 5, Small-inducible cytokine A5, T-cell-specific protein RANTES

5 Images

Sandwich ELISA - Monkey RANTES (CCL5) ELISA Kit (AB323522)

sELISA

Supplier Data

Sandwich ELISA - Monkey RANTES (CCL5) ELISA Kit (AB323522)

Interpolated concentrations of Cynomolgus Macaque RANTES in treated and untreated PBMC supernatant.

Interpolated concentration of native RANTES was measured in duplicate at different sample concentrations. Undiluted samples are as follows : treated Cynomolgus Macaque PBMC supernatant (50 ng/mL PMA + 1 µg/mL ionomycin, 24 hrs) 1 : 100 and untreated Cynomolgus Macaque PBMC supernatant 1 : 2. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. No detectable RANTES concentrations were measured in 100% cell culture media (RPMI1640 containing 10% fetal bovine serum).

sELISA

Supplier Data

Sandwich ELISA - Monkey RANTES (CCL5) ELISA Kit (AB323522)

Example of monkey RANTES standard curve in Sample Diluent NS.

Example of monkey RANTES standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Monkey RANTES (CCL5) ELISA Kit (AB323522)

Interpolated concentrations of Rhesus Macaque RANTES in serum, plasma (citrate), and plasma (EDTA).

Interpolated concentration of native RANTES was measured in duplicate at different sample concentrations. Undiluted samples are as follows : Rhesus Macaque serum 1 : 400, Rhesus Macaque plasma (citrate) 1 : 50, and Rhesus Macaque plasma (EDTA) 1 : 33. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Monkey RANTES (CCL5) ELISA Kit (AB323522)

Interpolated concentrations of monkey RANTES in serum in 96-well vs. 384-well plates.

Interpolated concentration of native RANTES was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 400 Rhesus Macaque serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Monkey RANTES (CCL5) ELISA Kit (AB323522)

Example of monkey RANTES standard curve in Sample Diluent NS in 96-well vs. 384-well plate.

Example of monkey RANTES standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant

Reacts with

Monkey

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 13.423 pg/mL

Range

37.5 - 2400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Monkey RANTES (CCL5) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RANTES (CCL5) protein in Serum, Cit Plasma, EDTA Plasma, Cell culture supernatant. Quantitate Monkey RANTES (CCL5) with 13.423 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

Single-wash protocol reduces assay time to 90 minutes or less
High sensitivity, specificity and reproducibility from superior antibodies
Fully validated in biological samples
96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" } ]

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Recovery

[ { "sample": "Serum", "range": "108 - 116 %", "average": "= 113" }, { "sample": "Citrate plasma", "range": "111 - 114 %", "average": "= 112" }, { "sample": "EDTA Plasma", "range": "105 - 119 %", "average": "= 114" }, { "sample": "Cell culture supernatant", "range": "102 - 110 %", "average": "= 105" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.

See full target information CCL5

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websiteProtocolBooklet

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Product promise

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