Monkey RANTES (CCL5) ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey RANTES (CCL5) with a sensitivity of 13.423 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Monkey
- Range
- 37.5 - 2400 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 13.423 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
Alternative names
C-C motif chemokine 5, Small-inducible cytokine A5, T-cell-specific protein RANTES
What's included?
Monkey RANTES (CCL5) ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey RANTES (CCL5) with a sensitivity of 13.423 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Monkey
- Range
- 37.5 - 2400 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 13.423 pg/mL
- General recovery
- 113%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 6.5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 5.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 113 | Range 108 - 116 % |
Sample type Citrate plasma | Average % = 112 | Range 111 - 114 % |
Sample type EDTA Plasma | Average % = 114 | Range 105 - 119 % |
Sample type Cell culture supernatant | Average % = 105 | Range 102 - 110 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Monkey RANTES (CCL5) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RANTES (CCL5) protein in Serum, Cit Plasma, EDTA Plasma, Cell culture supernatant. Quantitate Monkey RANTES (CCL5) with 13.423 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
- Also available in a fully validated 384-well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Sandwich ELISA - Monkey RANTES (CCL5) ELISA Kit (ab323522)
Interpolated concentrations of monkey RANTES in serum in 96-well vs. 384-well plates.
Interpolated concentration of native RANTES was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:400 Rhesus Macaque serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Monkey RANTES (CCL5) ELISA Kit (ab323522)
Example of monkey RANTES standard curve in Sample Diluent NS in 96-well vs. 384-well plate.
Example of monkey RANTES standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Monkey RANTES (CCL5) ELISA Kit (ab323522)
Interpolated concentrations of Cynomolgus Macaque RANTES in treated and untreated PBMC supernatant.
Interpolated concentration of native RANTES was measured in duplicate at different sample concentrations. Undiluted samples are as follows: treated Cynomolgus Macaque PBMC supernatant (50 ng/mL PMA + 1 ?g/mL ionomycin, 24 hrs) 1:100 and untreated Cynomolgus Macaque PBMC supernatant 1:2. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. No detectable RANTES concentrations were measured in 100% cell culture media (RPMI1640 containing 10% fetal bovine serum).
Sandwich ELISA - Monkey RANTES (CCL5) ELISA Kit (ab323522)
Interpolated concentrations of Rhesus Macaque RANTES in serum, plasma (citrate), and plasma (EDTA).
Interpolated concentration of native RANTES was measured in duplicate at different sample concentrations. Undiluted samples are as follows: Rhesus Macaque serum 1:400, Rhesus Macaque plasma (citrate) 1:50, and Rhesus Macaque plasma (EDTA) 1:33. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Monkey RANTES (CCL5) ELISA Kit (ab323522)
Example of monkey RANTES standard curve in Sample Diluent NS.
Example of monkey RANTES standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
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