AB282298

Monkey TIM-3 ELISA Kit

Recombinant
SimpleStep
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Monkey TIM-3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey TIM-3 with a sensitivity of 7.77 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

3 Images

Sandwich ELISA - Monkey TIM-3 ELISA Kit (AB282298)

sELISA

Supplier Data

Sandwich ELISA - Monkey TIM-3 ELISA Kit (AB282298)

Interpolated concentrations of native TIM-3 in monkey serum, plasma-EDTA and cell culture supernatant samples.

The concentrations of TIM-3 were measured in duplicates, interpolated from the TIM-3 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (EDTA) 50%, and PBMC stimulated supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIM-3 concentration was determined to be 662.6 pg/mL in serum, 1,663 pg/mL in plasma (EDTA), 205.2 pg/mL in stimulated PBMC supernatant, and undetectable in unstimulated PBMC supernatant.

sELISA

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Sandwich ELISA - Monkey TIM-3 ELISA Kit (AB282298)

Example of monkey TIM-3 standard curve in Sample Diluent NS + 1X Enhancer.

The TIM-3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Monkey TIM-3 ELISA Kit (AB282298)

Representative image of Monkey TIM-3 ELISA Kit (ab282298)

Components shown from left to right :

- Sample diluent NS

- 10X Monkey TIM-3 Capture Antibody

- 10X Monkey TIM-3 Detector Antibody

- Monkey TIM-3 Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Monkey

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 7.77 pg/mL

Range

43.75 - 2800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Monkey TIM-3 ELISA kit (ab272199) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Monkey TIM-3 protein in monkey srum, plasma, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey TIM-3 with 7.77 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

T-Cell Membrane Protein 3 (TIM-3), also known as Hepatitis A Virus Cellular Receptor 2 is type 1 transmembrane protein encoded by the HAVCR2 gene. TIM-3 contains a 181- amino acid extracellular portion that includes a Ig-like V-type domain, a 21- amino acid- transmembrane section, and a 78- amino acid cytoplasmic section. TIM-3 is a cell surface receptor expressed in Th1 lymphocytes, regulatory T-cells, innate immune cells including dendritic cells, NK cells, and monocytes. Binding to Galectin-9 induces intracellular calcium influx which triggers apoptosis. Other ligands are phosphatidyl serine, High Mobility Group Protein 1 (HMGB1), and Carcinoembryonic Antigen Related Cell Adhesion Molecule 1 (CEACAM1). TIM-3 can be cleaved to a soluble form from the cell surface by ADAM10 or ADAM17 mediated lysis. The soluble form of TIM-3 has been found to be elevated in the plasma of patients with HIV/AIDS, Hepatitis, and graft-versus-host Disease.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.79" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "96 - 99 %", "average": "= 100" }, { "sample": "Serum", "range": "90 - 93 %", "average": "= 92" }, { "sample": "EDTA Plasma", "range": "96 - 102 %", "average": "= 100" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab282298-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB282298-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB282298-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB282298-CMP11", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB282298-CMP12", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB282298-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB282298-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB282298-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Monkey TIM-3 Lyophilized Recombinant Protein", "number":"AB282298-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB282298-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey TIM-3 Capture Antibody", "number":"AB282298-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey TIM-3 Detector Antibody", "number":"AB282298-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIM-3 also known as T cell immunoglobulin and mucin-domain containing-3 or HAVCR2 is a protein involved in immune regulation. It possesses an approximate mass of 35 kDa. TIM-3 is expressed on various immune cells including T cells NK cells and dendritic cells especially after activation. The expression level often changes in response to inflammatory conditions suggesting its role in modulating immune responses.

Biological function summary

TIM-3 functions as a checkpoint inhibitor impacting immune cell activity. It is not part of a larger physical complex but it modulates immune responses by interacting with its ligands such as Galectin-9 phosphatidylserine and CEACAM1. TIM-3 involvement in downregulating Th1 cell responses shows its necessary role in maintaining immune homeostasis. The protein also acts in regulating tolerance mechanisms and preventing autoimmunity.

Pathways

TIM-3 participation is seen in the immune checkpoint and T cell exhaustion pathways. TIM-3 signaling results in T cell inhibition affecting the PD-1 pathway as well. It shares a relationship with proteins like LAG-3 and PD-1 which are key to immune inhibitory signaling. These interactions depict TIM-3's role in immune tolerance during chronic infections and malignancies.

TIM-3 association with cancer and chronic infections provides insight into therapeutic implications. In cancer TIM-3 contributes to immune evasion often co-expressed with PD-1 leading to T cell exhaustion. In autoimmune diseases TIM-3 modulation may affect disease progression by influencing immune tolerance. Understanding TIM-3's role in these contexts aids in developing targeted therapies such as anti-TIM-3 antibodies to enhance immune responses in cancer while promoting tolerance in autoimmunity.

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Product protocols

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Target data

See full target information HAVCR2

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Monkey TIM-3 ELISA Kit

Sandwich ELISA - Monkey TIM-3 ELISA Kit (AB282298)

Sandwich ELISA - Monkey TIM-3 ELISA Kit (AB282298)

Sandwich ELISA - Monkey TIM-3 ELISA Kit (AB282298)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Secondary Antibodies

Primary Antibodies

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise