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AB252354

Monkey TNF alpha ELISA Kit

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(4 Publications)

Monkey TNF alpha ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey TNF alpha with a sensitivity of 4.93 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

4 Images
ELISA - Monkey TNF alpha ELISA Kit (AB252354)
  • ELISA

Supplier Data

ELISA - Monkey TNF alpha ELISA Kit (AB252354)
Sandwich ELISA - Monkey TNF alpha ELISA Kit (AB252354)
  • sELISA

Unknown

Sandwich ELISA - Monkey TNF alpha ELISA Kit (AB252354)

Example of monkey TNF alpha standard curve in Sample Diluent NS.

The TNF alpha standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Monkey TNF alpha ELISA Kit (AB252354)
  • sELISA

Unknown

Sandwich ELISA - Monkey TNF alpha ELISA Kit (AB252354)

Interpolated concentrations of native TNF alpha in Rhesus macaque PHA stimulated cell culture supernatant samples.

The concentrations of TNF alpha were measured in duplicates, interpolated from the TNF alpha standard curves and corrected for sample dilution. Undiluted samples are as follows : PHA stimulated PBMC supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNF alpha concentration was determined to be 5776.09 pg/mL in PHA stimulated PBMC supernatant.

Sandwich ELISA - Monkey TNF alpha ELISA Kit (AB252354)
  • sELISA

Unknown

Sandwich ELISA - Monkey TNF alpha ELISA Kit (AB252354)

Interpolated concentrations of native TNF alpha in Rhesus macaque PHA stimulated and unstimulated cell culture supernatant samples.

The concentrations of TNF alpha were measured in duplicates, interpolated from the TNF alpha standard curves and corrected for sample dilution. Undiluted samples are as follows : unstimulated PBMC supernatant 12.5% and PHA stimulated PBMC supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNF alpha concentration was determined to be under the minimal detectable dose for unstimulated PBMC supernatant and 5776.09 pg/mL in PHA stimulated PBMC supernatant.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Cerebral Spinal Fluid, Heparin Plasma, Serum

Reacts with

Monkey

Assay type

Sandwich (quantitative)

Sensitivity

= 4.93 pg/mL

Range

15.63 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Monkey TNF alpha ELISA Kit ab252354 is a rapid single-wash 90-min Sandwich ELISA to measure Monkey TNF alpha in cell culture supernatant, CSF, heparin plasma, serum. This SimpleStep sensitivity is 4.93 pg/mL.

How the assay works

Monkey TNF alpha SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Monkey TNF alpha ELISA Kit ab252354 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]
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Recovery

[ { "sample": "Cerebral Spinal Fluid", "range": "94 - 102 %", "average": "= 99" }, { "sample": "Cell culture supernatant", "range": "91 - 119 %", "average": "= 106" }, { "sample": "Serum", "range": "80 - 82 %", "average": "= 81" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab252354-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB252354-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB252354-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB252354-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB252354-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB252354-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey TNF alpha Capture Antibody", "number":"AB252354-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Monkey TNF alpha Lyophilized Recombinant Protein", "number":"AB252354-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB252354-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey TNF alpha Detector Antibody", "number":"AB252354-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB252354-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
See full target information TNF
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 23:258 PubMed40158134

2025

Single-cell multi-omics analysis reveals the mechanism of action of a novel antioxidant polyphenol nanoparticle loaded with STAT3 agonist in mediating cardiomyocyte ferroptosis to ameliorate age-related heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Haoyuan Zheng,Yuan Tian,Dongyu Li,Yanxiao Liang

NPJ vaccines 9:153 PubMed39164276

2024

A multi-targeting immunotherapy ameliorates multiple facets of Alzheimer's disease in 3xTg mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xuejian Feng,Yunyu Hou,Jiaxin Liu,Fei Yan,Mingrui Dai,Mo Chen,Jianan Wang,Jie Li,Zhenjiang Liu,Dong Sun,Yong Zhang,Xianghui Yu,Wei Kong,Hui Wu

Frontiers in aging neuroscience 14:918640 PubMed35847680

2022

The Effect of Sevoflurane Anesthesia on the Biomarkers of Neural Injury in the Prefrontal Cortex of Aged Marmosets.

Applications

Unspecified application

Species

Unspecified reactive species

Yanyong Cheng,Lingling Shi,Haoli Mao,Zhenyu Xue,Siyu Liu,Zilong Qiu,Lei Zhang,Hong Jiang

Cell 185:1728-1744.e16 PubMed35460644

2022

Circular RNA vaccines against SARS-CoV-2 and emerging variants.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Qu,Zongyi Yi,Yong Shen,Liangru Lin,Feng Chen,Yiyuan Xu,Zeguang Wu,Huixian Tang,Xiaoxue Zhang,Feng Tian,Chunhui Wang,Xia Xiao,Xiaojing Dong,Li Guo,Shuaiyao Lu,Chengyun Yang,Cong Tang,Yun Yang,Wenhai Yu,Junbin Wang,Yanan Zhou,Qing Huang,Ayijiang Yisimayi,Shuo Liu,Weijin Huang,Yunlong Cao,Youchun Wang,Zhuo Zhou,Xiaozhong Peng,Jianwei Wang,Xiaoliang Sunney Xie,Wensheng Wei
View all publications
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websiteProtocolBooklet
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