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Monkey TNF alpha ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Monkey TNF alpha in Cell culture supernatant, Cerebral Spinal Fluid, Heparin Plasma, Serum samples.
Alternative names=Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, TNFSF2, TNFA, TNF
- Detection method
Colorimetric
- Sample types
Cell culture supernatant, Cerebral Spinal Fluid, Heparin Plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Monkey
- Range
15.63 - 1000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 4.93 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Images
Target data
Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity).The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
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Monkey TNF alpha ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Monkey TNF alpha in Cell culture supernatant, Cerebral Spinal Fluid, Heparin Plasma, Serum samples.
Alternative names=Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, TNFSF2, TNFA, TNF
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture supernatant, Cerebral Spinal Fluid, Heparin Plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Monkey
- Range
15.63 - 1000 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 4.93 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 8 | mean - | SD - | C.V. 3.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 3 | mean - | SD - | C.V. 3.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cerebral Spinal Fluid | Average % = 99 | Range 94 - 102 % |
Sample type Cell culture supernatant | Average % = 106 | Range 91 - 119 % |
Sample type Serum | Average % = 81 | Range 80 - 82 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Monkey TNF alpha ELISA Kit (ab252354) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TNF alpha protein in cell culture supernatant, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey TNF alpha with 4.93 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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