Monkey TNF alpha ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey TNF alpha with a sensitivity of 4.93 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Frontiers in aging neuroscience 14:9186402022The Effect of Sevoflurane Anesthesia on the Biomarkers of Neural Injury in the Prefrontal Cortex of Aged Marmosets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanyong Cheng,Lingling Shi,Haoli Mao,Zhenyu Xue,Siyu Liu,Zilong Qiu,Lei Zhang,Hong JiangPubMed 35847680
- Cell 185:1728-1744.e162022Circular RNA vaccines against SARS-CoV-2 and emerging variants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Qu,Zongyi Yi,Yong Shen,Liangru Lin,Feng Chen,Yiyuan Xu,Zeguang Wu,Huixian Tang,Xiaoxue Zhang,Feng Tian,Chunhui Wang,Xia Xiao,Xiaojing Dong,Li Guo,Shuaiyao Lu,Chengyun Yang,Cong Tang,Yun Yang,Wenhai Yu,Junbin Wang,Yanan Zhou,Qing Huang,Ayijiang Yisimayi,Shuo Liu,Weijin Huang,Yunlong Cao,Youchun Wang,Zhuo Zhou,Xiaozhong Peng,Jianwei Wang,Xiaoliang Sunney Xie,Wensheng WeiPubMed 35460644
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Cerebral Spinal Fluid, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Monkey
- Range
- 15.63 - 1000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 4.93 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity). The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Alternative names
TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a
What's included?
Recommended products
Monkey TNF alpha ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey TNF alpha with a sensitivity of 4.93 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Cerebral Spinal Fluid, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Monkey
- Range
- 15.63 - 1000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 4.93 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Supernatant | n 8 | C.V. 3.6 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Supernatant | n 3 | C.V. 3.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cerebral Spinal Fluid | Average % = 99 | Range 94 - 102 % |
Sample type Cell culture supernatant | Average % = 106 | Range 91 - 119 % |
Sample type Serum | Average % = 81 | Range 80 - 82 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Monkey TNF alpha ELISA Kit (ab252354) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TNF alpha protein in cell culture supernatant, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey TNF alpha with 4.93 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Sandwich ELISA - Monkey TNF alpha ELISA Kit (ab252354)
Example of monkey TNF alpha standard curve in Sample Diluent NS.
The TNF alpha standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Monkey TNF alpha ELISA Kit (ab252354)
Interpolated concentrations of native TNF alpha in Rhesus macaque PHA stimulated cell culture supernatant samples.
The concentrations of TNF alpha were measured in duplicates, interpolated from the TNF alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: PHA stimulated PBMC supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNF alpha concentration was determined to be 5776.09 pg/mL in PHA stimulated PBMC supernatant.
Sandwich ELISA - Monkey TNF alpha ELISA Kit (ab252354)
Interpolated concentrations of native TNF alpha in Rhesus macaque PHA stimulated and unstimulated cell culture supernatant samples.
The concentrations of TNF alpha were measured in duplicates, interpolated from the TNF alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated PBMC supernatant 12.5% and PHA stimulated PBMC supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNF alpha concentration was determined to be under the minimal detectable dose for unstimulated PBMC supernatant and 5776.09 pg/mL in PHA stimulated PBMC supernatant.
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