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AB213843

Mouse ACE2 ELISA Kit

5

(1 Review)

|

(7 Publications)

Mouse ACE2 ELISA Kit is a Sandwich ELISA for the measurement of Mouse ACE2 in Mouse in Cell Culture Media, Biofluids samples.

View Alternative Names

Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, Ace2

1 Images
Sandwich ELISA - Mouse ACE2 ELISA Kit (AB213843)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse ACE2 ELISA Kit (AB213843)

Mouse ACE2 ELISA Kit (ab213843) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Heparin Plasma, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Mouse ACE2 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213843) is designed for the quantitative measurement of Mouse ACE2 in cell culture supernatants, serum and plasma (heparin).

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for ACE2 has been pre-coated onto 96-well plates. Standards (Expression system for standard: CHO; Immunogen sequence: Q18-T740) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ACE2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse ACE2 amount of sample captured in plate.

Angiotensin-converting enzyme 2 (ACE 2) is a protein belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. By sequence similarity to a sequence in GenBank, this gene is mapped to Xp22.2. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "131 pg/mL", "standardDeviation": "10.48", "coefficientOfVariability": "8" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "557 pg/mL", "standardDeviation": "31.74", "coefficientOfVariability": "5.7" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "1192 pg/mL", "standardDeviation": "84.63", "coefficientOfVariability": "7.1" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "146 pg/mL", "standardDeviation": "11.38", "coefficientOfVariability": "7.8" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "538 pg/mL", "standardDeviation": "27.88", "coefficientOfVariability": "4.7" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "1252 pg/mL", "standardDeviation": "75.77", "coefficientOfVariability": "6.1" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ACE2 protein also known as angiotensin-converting enzyme 2 is an essential component in the renin-angiotensin system. It functions mechanically by converting the hormone angiotensin II to angiotensin-(1-7) which helps regulate blood pressure and fluid balance. The molecular weight of ACE2 is approximately 120 kDa. This protein is expressed in various tissues particularly the lungs heart kidneys and gastrointestinal tract. In cultured cells like Caco-2 cells researchers often study this expression.
Biological function summary

The ACE2 protein plays an important role in the regulation of cardiovascular and renal functions. It is a single-pass type I membrane protein and its activity reduces inflammation and oxidative stress in cells. ACE2 does not function as part of a larger protein complex but its enzymatic conversion has a substantial impact on reducing the effects of angiotensin II in the body leading to vasodilation and decreased blood pressure.

Pathways

ACE2 involvement is significant in the renin-angiotensin system and the kallikrein-kinin system. These pathways are essential for maintaining cardiovascular homeostasis. In the renin-angiotensin system ACE2 works in opposition to angiotensin-converting enzyme (ACE) balancing the effects through the production of angiotensin-(1-7) from angiotensin II. Additionally ACE2 interacts indirectly with proteins like angiotensin receptor type 1 (AT1) and angiotensin receptor type 2 (AT2) ensuring proper signaling and physiological responses.

ACE2 links closely with conditions such as hypertension and COVID-19. Increased activity of angiotensin II due to low ACE2 levels contributes to hypertension. In infectious disease SARS-CoV-2 virus responsible for COVID-19 uses ACE2 as an entry receptor to initiate infection in host cells. This interaction highlights the importance of ACE2 in disease pathogenesis and has prompted interest in ACE2 as a potential therapeutic target.
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Product protocols

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Target data

Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II. Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin. Also cleaves other biological peptides, such as apelins, casomorphins and dynorphin A (By similarity). By cleavage of angiotensin II, may be an important regulator of heart function (PubMed : 12075344, PubMed : 12967627). By cleavage of angiotensin II, may also have a protective role in acute lung injury (PubMed : 16001071). Plays an important role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19, regulating its trafficking on the cell surface and its activity (PubMed : 18424768, PubMed : 19185582, PubMed : 22677001).
See full target information Ace2
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed34575910

2021

Cardiac and Renal SARS-CoV-2 Viral Entry Protein Regulation by Androgens and Diet: Implications for Polycystic Ovary Syndrome and COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Samar Rezq,Alexandra M Huffman,Jelina Basnet,Licy L Yanes Cardozo,Damian G Romero

International journal of molecular sciences 22: PubMed33922918

2021

SARS-CoV-2 Viral Entry Proteins in Hyperandrogenemic Female Mice: Implications for Women with PCOS and COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra M Huffman,Samar Rezq,Jelina Basnet,Licy L Yanes Cardozo,Damian G Romero

Environmental toxicology and pharmacology 86:103656 PubMed33838329

2021

E-cigarette vape and lung ACE2 expression: Implications for coronavirus vulnerability.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Lallai,Letizia Manca,Christie D Fowler

Clinical and experimental hypertension (New York, 42:661-668 PubMed32476477

2020

Chemical renal denervation-induced upregulation of the ACE2/Ang (1-7)/Mas axis attenuates blood pressure elevation in spontaneously hypertensive rats.

Applications

Unspecified application

Species

Unspecified reactive species

Wenzheng Han,Ming Wang,Xinrong Zhai,Qian Gan,Shaofeng Guan,Xinkai Qu

Acta neuropathologica 139:485-502 PubMed31982938

2020

ACE2 activation protects against cognitive decline and reduces amyloid pathology in the Tg2576 mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Charles E Evans,James S Miners,Giulia Piva,Christine L Willis,David M Heard,Emma J Kidd,Mark A Good,Patrick G Kehoe

Cell metabolism 27:1323-1337.e5 PubMed29706566

2018

FGF21 Prevents Angiotensin II-Induced Hypertension and Vascular Dysfunction by Activation of ACE2/Angiotensin-(1-7) Axis in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xuebo Pan,Yihui Shao,Fan Wu,Yuan Wang,Rongrong Xiong,Jujia Zheng,Haishan Tian,Baile Wang,Yanfang Wang,Yi Zhang,Zongsheng Han,Aijuan Qu,Haixia Xu,Aihua Lu,Tianxin Yang,Xiaokun Li,Aimin Xu,Jie Du,Zhuofeng Lin

Molecular medicine reports 16:8485-8492 PubMed28944898

2017

Effects of IQP, VEP and Spirulina platensis hydrolysates on the local kidney renin angiotensin system in spontaneously hypertensive rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahui Zheng,Jingyue Wang,Huanglei Pan,Hongli Wu,Difeng Ren,Jun Lu
View all publications
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