Mouse Activin A ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse Activin A in Mouse in Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 62.5 - 4000 pg/mL
- Sensitivity
- < 10 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
Alternative names
Inhibin beta A chain, Activin beta-A chain, Inhba
What's included?
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Mouse Activin A ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse Activin A in Mouse in Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 62.5 - 4000 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 10 pg/mL
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's mouse Activin A in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse Activin A in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.
A Activin A specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Activin A is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Activin A amount of sample captured in plate.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Activin A also known by its gene name INHBA is a protein involved in various mechanical processes in the human body. This protein forms part of the transforming growth factor-beta (TGF-β) superfamily and has a molecular weight of approximately 25 kDa for its dimeric forms. Activin A is expressed in many tissues including the pituitary gland reproductive organs and placenta. Researchers often use techniques like the Activin A ELISA test kit to measure its concentration in biological samples.
- Biological function summary
Activin A plays a significant role in regulating cell differentiation proliferation and apoptosis. As part of a complex Activin A interacts with specific receptors on cell surfaces initiating intracellular signaling cascades that influence these vital processes. It acts as a growth factor in the reproductive system influencing follicle-stimulating hormone (FSH) secretion and is important in embryonic development and wound healing.
- Pathways
Activin A participates in pathways such as the TGF-β signaling pathway and the SMAD protein signaling pathway. Within these pathways it interacts with receptor proteins like type I and type II Activin receptors and downstream SMAD proteins. These interactions enable Activin A to regulate gene expression relevant to various cellular functions.
- Associated diseases and disorders
Altered Activin A levels have associations with conditions like cancer and fibrotic diseases. Its overexpression links to some cancer types including colorectal and prostate cancer where it influences tumor growth and metastasis. Moreover its interaction with proteins like myostatin may contribute to muscle wasting disorders. The dysregulation of these pathways highlights the significance of Activin A in pathophysiology.
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1 product image
Sandwich ELISA - Mouse Activin A ELISA Kit (ab119569)
Representative Standard Curve using ab199569.
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Product protocols
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