Mouse Adiponectin ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Mouse Adiponectin with a sensitivity of 7.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Heparin Plasma, Citrate plasma, EDTA Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 23.44 - 1500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 7.5 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
Alternative names
Acdc, Acrp30, Apm1, Adipoq, Adiponectin, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipocyte-specific protein AdipoQ, ACRP30
What's included?
Recommended products
Mouse Adiponectin ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Mouse Adiponectin with a sensitivity of 7.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Heparin Plasma, Citrate plasma, EDTA Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 23.44 - 1500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 7.5 pg/mL
- General recovery
- 112%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 2.4 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 2.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 105 | Range 103 - 107 % |
Sample type Citrate plasma | Average % = 91 | Range 84 - 95 % |
Sample type EDTA Plasma | Average % = 94 | Range 91 - 96 % |
Sample type Heparin Plasma | Average % = 95 | Range 87 - 101 % |
Sample type Serum | Average % = 112 | Range 109 - 114 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse Adiponectin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Adiponectin protein in mouse serum, plasma and cell culture supernatant. Quantitate Mouse Adiponectin with 2.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Adiponectin also known as ADIPOQ or adipocyte complement-related protein of 30 kDa (Acrp30) is a 30 kDa protein that plays a significant mechanical role in energy homeostasis. Adiponectin is secreted predominantly by adipose tissue and circulates in the blood. This protein forms various multimeric structures including low-molecular-weight medium-molecular-weight and high-molecular-weight forms each with distinct biological functions. Its expression in adipose tissue links it closely to metabolic processes and functions related to fat storage and energy balance.
- Biological function summary
Adiponectin influences glucose regulation and fatty acid oxidation. It acts as a hormone with several metabolic roles including anti-diabetic anti-atherogenic and anti-inflammatory properties. Adiponectin participates in forming a complex with other proteins such as AdipoR1 and AdipoR2 which are receptors facilitating signal transduction. The interaction of adiponectin with its receptors leads to the induction of several lipid and glucose metabolism pathways important for maintaining cellular energy balance.
- Pathways
Many regulatory cascades are influenced by adiponectin. This protein is integral to the AMPK signaling pathway and the PPAR signaling pathway. In the AMPK pathway adiponectin enhances insulin sensitivity and stimulates oxidation of fatty acids in muscle tissue. Through the PPAR pathway it influences lipid metabolism and storage. Adiponectin interacts with key proteins such as leptin and resistin coordinating various metabolic pathways to balance energy and glucose levels making it a critical factor in metabolic regulation.
- Associated diseases and disorders
Reduced levels of adiponectin associate with conditions like type 2 diabetes and cardiovascular disease. Lower circulating adiponectin concentrations correlate with obesity leading to increased insulin resistance and higher risk of type 2 diabetes. In cardiovascular disease this protein's role links to its anti-inflammatory properties and ability to decrease the proliferation of vascular smooth muscle cells. Adiponectin also interacts with proteins like cytokines that play a role in these conditions affecting the body’s inflammatory responses and metabolic processes highlighting its importance in health and disease management.
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5 product images
Sandwich ELISA - Mouse Adiponectin ELISA Kit - Extracellular (ab309173)
Example of mouse Adiponectin standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Adiponectin ELISA Kit - Extracellular (ab309173)
Interpolated concentration of native Adiponectin was measured in duplicate at different sample concentrations. Undiluted samples are as follows: 1:10,000 mouse serum, 1:10,000 plasma (citrate), 1:10,000 plasma (EDTA), and 1:10,000 plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Mouse Adiponectin ELISA Kit - Extracellular (ab309173)
Interpolated concentration of native Adiponectin was measured in duplicate at different sample concentrations. Undiluted samples are as follows: mouse kidney supernatant 3%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Mouse Adiponectin ELISA Kit - Extracellular (ab309173)
Standard curve comparison between the original Mouse Adiponectin SimpleStep ELISA (Mouse Adiponectin ELISA Kit ab226900) and current Mouse Adiponectin SimpleStep ELISA (ab309173).
Sandwich ELISA - Mouse Adiponectin ELISA Kit - Extracellular (ab309173)
Standard curve comparison between Mouse Adiponectin SimpleStep ELISA kit (ab309173) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.
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