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AB245718

Mouse AGT ELISA Kit

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(4 Publications)

Mouse AGT ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse AGT with a sensitivity of 49.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Serpina8, Agt, Angiotensinogen, Serpin A8

4 Images
ELISA - Mouse AGT ELISA Kit (AB245718)
  • ELISA

Supplier Data

ELISA - Mouse AGT ELISA Kit (AB245718)
Sandwich ELISA - Mouse AGT ELISA Kit (AB245718)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse AGT ELISA Kit (AB245718)

Interpolated concentrations of native AGT in mouse serum and plasma samples.

The concentrations of AGT were measured in duplicates, interpolated from the AGT standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 2,000, plasma (citrate) 1 : 2,000, plasma (EDTA) 1 : 2,000, and plasma (heparin) 1 : 2,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AGT concentration was determined to be 13.4 μg/mL in serum, 9.1 μg/mL in plasma (citrate), 8.8 μg/mL in plasma (EDTA), and 12.5 μg/mL in plasma (heparin).

Sandwich ELISA - Mouse AGT ELISA Kit (AB245718)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse AGT ELISA Kit (AB245718)

Interpolated concentrations of native AGT in mouse liver supernatant samples. The concentrations of AGT were measured in duplicates, interpolated from the AGT standard curves and corrected for sample dilution. Undiluted samples are as follows : mouse liver supernatant 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AGT concentration was determined to be 70.7 ng/mL in mouse liver supernatant.

Sandwich ELISA - Mouse AGT ELISA Kit (AB245718)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse AGT ELISA Kit (AB245718)

Example of mouse AGT standard curve in Sample Diluent NS. The AGT standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 49.9 pg/mL

Range

156.25 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse AGT ELISA Kit ab245718 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse AGT in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 49.9 pg/mL.

How the assay works

Mouse AGT SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse AGT ELISA Kit ab245718 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Angiotensinogen antibody [EPR22199-244] - BSA and Azide free (Capture) ab259566
- Anti-Angiotensinogen antibody [EPR22199-236] - BSA and Azide free (Detector) ab259566
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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "99 - 105 %", "average": "= 103" }, { "sample": "Serum", "range": "107 - 115 %", "average": "= 112" }, { "sample": "EDTA Plasma", "range": "105 - 115 %", "average": "= 111" }, { "sample": "Heparin Plasma", "range": "92 - 112 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "110 - 114 %", "average": "= 112" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab245718-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB245718-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB245718-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB245718-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB245718-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB245718-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse AGT Lyophilized Recombinant Protein", "number":"AB245718-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse AGT Capture Antibody", "number":"AB245718-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB245718-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse AGT Detector Antibody", "number":"AB245718-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB245718-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiotensinogen also known as AGT is a precursor to angiotensin peptides and plays a mechanical role in the renin-angiotensin system. It is a glycoprotein with a mass of approximately 60 kDa and is mainly produced in the liver. This protein circulates in the bloodstream where it waits for renin to cleave it. Angiotensinogen's presence is significant in various tissues but primarily it functions within the plasma after hepatic secretion.
Biological function summary

Angiotensinogen is critical in the regulation of blood pressure and fluid balance. It serves as the substrate for renin an enzyme that initiates a cascade leading to the production of angiotensin I which further converts into active forms like angiotensin II. Angiotensinogen does not operate as part of a larger protein complex but interacts closely with enzymes and other substrates to mediate its effects on the cardiovascular system.

Pathways

Angiotensinogen is an essential component of the renin-angiotensin-aldosterone system (RAAS) which helps regulate blood pressure and electrolyte balance. The pathway involves the conversion of angiotensinogen to angiotensin I by renin followed by the conversion to angiotensin II by angiotensin-converting enzyme (ACE). This system also has connections with proteins such as aldosterone which is involved in sodium and water retention influencing cardiovascular health.

Angiotensinogen links to hypertension and cardiovascular diseases. The protein's interaction with renin and ACE results in the formation of angiotensin II a potent vasoconstrictor that increases blood pressure. Elevated levels of angiotensinogen are often observed in individuals with high 117/85 blood pressure indicating a risk for chronic conditions. Managing angiotensinogen levels through RAAS modulation is a therapeutic strategy for controlling these disorders.
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Product protocols

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Target data

Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.. Angiotensin-2. Acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone. Acts by binding to angiotensin receptors AGTR1 and AGTR2. Also binds the DEAR/FBXW7-AS1 receptor.. Angiotensin-3. Stimulates aldosterone release.. Angiotensin 1-7. Is a ligand for the G-protein coupled receptor MAS1 (PubMed : 12829792). Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets (PubMed : 12829792, PubMed : 18026570).
See full target information Agt
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Foods (Basel, Switzerland) 13: PubMed38790847

2024

Modulatory Effects of the Kuwanon-Rich Fraction from Mulberry Root Bark on the Renin-Angiotensin System.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Hae Lee,Heon-Woong Kim,So-Ah Kim,Wan-Taek Ju,Seong-Ryul Kim,Hyun-Bok Kim,Ik-Seob Cha,Seong-Wan Kim,Jong-Woo Park,Sang-Kuk Kang

Global translational medicine 2: PubMed37293374

2023

Antisense oligonucleotides targeting hepatic angiotensinogen reduce atherosclerosis and liver steatosis in hypercholesterolemic mice.

Applications

Unspecified application

Species

Mouse

Dien Ye,Congqing Wu,Lei Cai,Deborah A Howatt,Ching-Ling Liang,Yuriko Katsumata,Adam E Mullick,Ryan E Temel,A H Jan Danser,Alan Daugherty,Hong S Lu

JCI insight 8: PubMed36472907

2023

LRP1 protects against excessive superior mesenteric artery remodeling by modulating angiotensin II-mediated signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jackie M Zhang,Dianaly T Au,Hisashi Sawada,Michael K Franklin,Jessica J Moorleghen,Deborah A Howatt,Pengjun Wang,Brittany O Aicher,Brian Hampton,Mary Migliorini,Fenge Ni,Adam E Mullick,Mashhood M Wani,Areck A Ucuzian,Hong S Lu,Selen C Muratoglu,Alan Daugherty,Dudley K Strickland

Arteriosclerosis, thrombosis, and vascular biology 42:1254-1261 PubMed36004642

2022

Inhibition of the Renin-Angiotensin System Fails to Suppress β-Aminopropionitrile-Induced Thoracic Aortopathy in Mice-Brief Report.

Applications

Unspecified application

Species

Mouse

Hisashi Sawada,Satoko Ohno-Urabe,Dien Ye,Michael K Franklin,Jessica J Moorleghen,Deborah A Howatt,Adam E Mullick,Alan Daugherty,Hong S Lu
View all publications
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