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AB326314

Mouse AGT ELISA Kit, Chemiluminescent

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Mouse AGT ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse AGT with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 22.86 - 50000 pg/ml with a sensitivity of 7.5pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

Serpina8, Agt, Angiotensinogen, Serpin A8

5 Images
Sandwich ELISA - Mouse AGT ELISA Kit, Chemiluminescent (AB326314)
  • sELISA

Lab

Sandwich ELISA - Mouse AGT ELISA Kit, Chemiluminescent (AB326314)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Mouse AGT ELISA Kit, Chemiluminescent (AB326314)
  • sELISA

Lab

Sandwich ELISA - Mouse AGT ELISA Kit, Chemiluminescent (AB326314)

Standard curve comparison between the Chemiluminescent Mouse AGT SimpleStep ELISA and colorimetric Mouse AGT SimpleStep ELISA (ab245718) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse AGT ELISA Kit, Chemiluminescent (AB326314)
  • sELISA

Lab

Sandwich ELISA - Mouse AGT ELISA Kit, Chemiluminescent (AB326314)

Dynamic range comparison between the Chemiluminescent Mouse AGT SimpleStep ELISA, colorimetric Mouse AGT SimpleStep ELISA (ab245718), and leading chemiluminescent competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Mouse AGT ELISA Kit, Chemiluminescent (AB326314)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse AGT ELISA Kit, Chemiluminescent (AB326314)

Interpolated concentrations of native AGT in mouse serum and plasma samples.

The concentrations of AGT were measured in duplicates interpolated from the AGT standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 2000 plasma (citrate) 1 : 2000 plasma (EDTA) 1 : 2000 and plasma (heparin) 1 : 2000. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean AGT concentration was determined to be 13.4 µg/mL in serum 9.1 µg/mL in plasma (citrate) 8.8 µg/mL in plasma (EDTA) and 12.5 µg/mL in plasma (heparin).

Sandwich ELISA - Mouse AGT ELISA Kit, Chemiluminescent (AB326314)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse AGT ELISA Kit, Chemiluminescent (AB326314)

Interpolated concentrations of native AGT in mouse liver supernatant samples. The concentrations of AGT were measured in duplicates interpolated from the AGT standard curves and corrected for sample dilution. Undiluted samples are as follows : mouse liver supernatant 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean AGT concentration was determined to be 70.7 ng/mL in mouse liver supernatant.

Key facts

Detection method

Luminescent

Sample types

Cell culture supernatant, Serum, EDTA Plasma, Heparin Plasma, Citrate plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 7.5 pg/mL

Range

22.86 - 50000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Mouse AGT ELISA Kit, Chemiluminescent (ab326314) is a rapid single-wash sandwich 55-min ELISA to measure Mouse AGT in Cell culture supernatant, Serum, EDTA Plasma, Heparin Plasma, Citrate plasma with a sensitivity of 7.5 pg/mL.

How the assay works

Mouse AGT ELISA Kit, Chemiluminescent (ab326314) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse AGT (ab326314) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-AGT antibody [EPR22199-244] - BSA and Aside free (Capture) ab259565
- Anti-AGT antibody [EPR22199-236] - BSA and Aside free (Detector) ab259566
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "99 - 105 %", "average": "= 103" }, { "sample": "Serum", "range": "107 - 115 %", "average": "= 112" }, { "sample": "EDTA Plasma", "range": "105 - 115 %", "average": "= 111" }, { "sample": "Heparin Plasma", "range": "92 - 112 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "110 - 114 %", "average": "= 112" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.. Angiotensin-2. Acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone. Acts by binding to angiotensin receptors AGTR1 and AGTR2. Also binds the DEAR/FBXW7-AS1 receptor.. Angiotensin-3. Stimulates aldosterone release.. Angiotensin 1-7. Is a ligand for the G-protein coupled receptor MAS1 (PubMed : 12829792). Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets (PubMed : 12829792, PubMed : 18026570).
See full target information Agt
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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