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AB285263

Mouse Alanine Aminotransferase (ALT) ELISA Kit

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(8 Publications)

Mouse Alanine Aminotransferase (ALT) ELISA Kit is a sandwich ELISA designed to quantify Mouse Alanine Aminotransferase (ALT) with a sensitivity of 0.938 mIU/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 1.56 - 100 mIU/mL

View Alternative Names

Gpt1, Gpt, Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT 1

1 Images
Sandwich ELISA - Mouse Alanine Aminotransferase (ALT) ELISA Kit (AB285263)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Alanine Aminotransferase (ALT) ELISA Kit (AB285263)

ALT Standard Curve.

Typical Standard Curve : These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 0.938 mIU/mL

Range

1.56 - 100 mIU/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Alanine Aminotransferase (ALT) ELISA Kit ab285263 is a sandwich ELISA to measure Mouse Alanine Aminotransferase (ALT) in serum, plasma, cell culture supernatant with a sensitivity of 0.938 0.

How the assay works

The density of color is proportional to the amount of mouse GPT captured from the samples.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse Alanine Aminotransferase (ALT) ELISA Kit ab285263 protocol summary

1. Wash plate with wash solution
2. Add standards and samples into appropriate wells. Incubate then discard
3. Add Biotin detection antibody and incubate,
4. Wash
Add SABC solution and incubate
5. Wash
6. Add TMB solution and incubate
7. Add Stop solution and read at 450 nm

This product is manufactured by BioVision, an Abcam company and was previously called E4324 Alanine Aminotransferase 1 (GPT) (Mouse) ELISA Kit. E4324-100 is the same size as the 96 test size of ab285263.

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Precision

[ { "reproducibilityType": "Intra", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 8" }, { "reproducibilityType": "Inter", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285263-96Test", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB285263-CMP04", "productcode":"" }, { "size":"1 x 5 Unit", "name":"Plate sealers", "number":"AB285263-CMP05", "productcode":"" }, { "size":"1 x 120 µL", "name":"Biotin/Detection antibody (Concentrated)", "number":"AB285263-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB285263-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Substrate", "number":"AB285263-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample and Standard Dilution Buffer", "number":"AB285263-CMP08", "productcode":"" }, { "size":"1 x 2 Vial", "name":"Lyophilized Standard", "number":"AB285263-CMP06", "productcode":"" }, { "size":"1 x 120 µL", "name":"HRP-Streptavidin Conjugate (SABC)", "number":"AB285263-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"Antibody dilution buffer", "number":"AB285263-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"SABC dilution buffer", "number":"AB285263-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"25X Wash buffer", "number":"AB285263-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alanine transaminase also known as ALT or alanine aminotransferase is an important enzyme in the body. It has a molecular mass of approximately 54 kDa and catalyzes the conversion between alanine and pyruvate by transferring an amino group. ALT is mainly expressed in the liver but also present in other tissues like the heart and muscles. It is often measured as 'ALT activity' in clinical settings to ascertain liver health. In laboratories an 'ALT assay' or 'ALT kit' can determine alanine transaminase activity.
Biological function summary

Alanine transaminase plays a critical role in amino acid metabolism and energy production. It facilitates the transamination process which is significant for the balance of amino acids and carbohydrates. ALT as a standalone enzyme does not form part of a protein complex but works closely with alanine aminotransferase reactions. It helps maintain the cellular nitrogen balance linking nitrogen metabolism with energy pathways.

Pathways

The role of alanine transaminase is to interconnect carbohydrate and amino acid metabolism. It participates in the gluconeogenesis pathway and the citric acid cycle important for energy production. Through its activity in these pathways it interacts with proteins like aspartate aminotransferase (AST) affecting the balance of amino and keto acids in cells.

Alanine transaminase levels indicate liver conditions such as hepatitis and liver cirrhosis. Elevated ALT activity suggests liver damage or inflammation. It also connects to conditions involving muscular injury. In these scenarios increased presence and activity of AST often accompany ALT providing a broader picture of the extent of tissue damage. Understanding the involvement of ALT in these pathways helps diagnose and monitor the progression of relevant health issues.
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Product protocols

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Target data

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).
See full target information Gpt
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in nutrition 11:1469952 PubMed39742098

2025

High-fat diet mouse model receiving L-glucose supplementations propagates liver injury.

Applications

Unspecified application

Species

Unspecified reactive species

Johnny Amer,Athar Amleh,Ahmad Salhab,Yuval Kolodny,Shira Yochelis,Baker Saffouri,Yossi Paltiel,Rifaat Safadi

Frontiers in pharmacology 15:1484278 PubMed39654627

2024

Osteopontin neutralization increases vitamin D receptors on NKT cells and ameliorates liver fibrosis by promoting their activity.

Applications

Unspecified application

Species

Unspecified reactive species

Johnny Amer,Ahmad Salhab,Enas Hussini,Rasha Shweiki,Iman Zahran,Mohammad Far

Cancer immunology research 12:1691-1702 PubMed39189923

2024

CAR T Cells Engineered to Secrete IFNκ Induce Tumor Ferroptosis via an IFNAR/STAT1/ACSL4 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yaoxin Gao,Shasha Liu,Yifan Huang,Hui Wang,Yuyu Zhao,Xuyang Cui,Yajing Peng,Feng Li,Yi Zhang

Chinese medical journal 137:222-231 PubMed38167245

2024

AZD1775 and anti-PD-1 antibody synergistically sensitize hepatoma to radiotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yichun Yin,Jian Wang,Junxuan Yi,Kaiyue Zhang,Zimeng Yin,Shunzi Jin,Baisong Zheng

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2305550 PubMed37828611

2023

A Ubiquitin-Dependent Switch on MEF2D Senses Pro-Metastatic Niche Signals to Facilitate Intrahepatic Metastasis of Liver Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Junyu Xiang,Ni Zhang,Aibei Du,Jinyang Li,Mengyun Luo,Yuzhu Wang,Meng Liu,Luming Yang,Xianfeng Li,Lin Wang,Qin Liu,Dongfeng Chen,Tao Wang,Xiu-Wu Bian,Zhong-Yi Qin,Li Su,Liangzhi Wen,Bin Wang

Frontiers in pharmacology 14:1219709 PubMed37614321

2023

The immune and metabolic treatment approach of using testosterone on mice models of liver injury.

Applications

Unspecified application

Species

Unspecified reactive species

Johnny Amer,Ahmad Salhab,Hadeel Snobar,Yazan Alhabil

Plants (Basel, Switzerland) 12: PubMed36678965

2023

The Ethanol Extracts of Leaves Ameliorate the Bone Loss via the Inhibition of Osteoclastogenesis in Osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yo-Seob Seo,HyangI Lim,Jeong-Yeon Seo,Kyeong-Rok Kang,Do Kyung Kim,Hyun-Hwa Lee,Deuk-Sil Oh,Jae-Sung Kim

Journal of cell communication and signaling 17:723-736 PubMed36508052

2022

Catalpol inhibits hepatic stellate cell activation by reducing the formation and changing the contents of hepatocyte-derived extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng-Yuan Xie,Heng-Wei Cao,Qing Wang,Hui Lu,Wen Du
View all publications
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Product promise

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