AB108791

Mouse Albumin ELISA Kit

Name: Mouse Albumin ELISA Kit (ab108791) | Abcam
Brand: Abcam Limited
SKU: AB108791

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(17 Publications)

Mouse Albumin ELISA Kit is a competitive ELISA designed to quantify Mouse Albumin with a sensitivity of 1.4 µg/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 1.563 - 100 µg/ml
- Cited in over 10 publications

View Alternative Names

Alb-1, Alb1, Alb, Albumin

3 Images

Competitive ELISA - Mouse Albumin ELISA Kit (AB108791)

cELISA

Unknown

Competitive ELISA - Mouse Albumin ELISA Kit (AB108791)

Albumin measured in mouse tissue lysates (expressed as per mg of extracted protein), with background signal subtracted (duplicates; +/- SD).

cELISA

Supplier Data

Competitive ELISA - Mouse Albumin ELISA Kit (AB108791)

Mouse Albumin ELISA Kit example standard curve – data provided for demonstration purposes only.

cELISA

Unknown

Competitive ELISA - Mouse Albumin ELISA Kit (AB108791)

Albumin measured in biological fluids, background signal subtracted (duplicates +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Mouse

Assay type

Competitive

Sensitivity

= 1.4 µg/mL

Range

1.563 - 100 µg/mL

Assay time

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Albumin ELISA kit (ab108791) is a competitive ELISA designed for the quantitative measurement of mouse Albumin levels in plasma and serum.

An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Albumin is added and then followed by washing with PBS or TBS buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Albumin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.6" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108791-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108791-CMP04", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108791-CMP09", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108791-CMP08", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108791-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Albumin Standard", "number":"AB108791-CMP06", "productcode":"" }, { "size":"1 x 1 Test", "name":"Albumin Microplate (12 x 8 wells)", "number":"AB108791-CMP05", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108791-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108791-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"2X Biotinylated Mouse Albumin (Lyophilized)", "number":"AB108791-CMP01", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.

Biological function summary

Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.

Pathways

More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.

Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.

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Product protocols

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Target data

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (By similarity). Does not prevent iron uptake by the bacterial siderophore aerobactin (By similarity).

See full target information Alb

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Stem cell reviews and reports 20:2215-2235 PubMed39259445

2024

Small Molecule-Mediated Stage-Specific Reprogramming of MSCs to Hepatocyte-Like Cells and Hepatic Tissue for Liver Injury Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Santosh Gupta,Akriti Sharma,Muthukumarassamy Rajakannu,Jovana Bisevac,Mohamed Rela,Rama Shanker Verma

Journal of cellular biochemistry 123:481-492 PubMed34908186

2021

Diabetic kidney disease-predisposing proinflammatory and profibrotic genes identified by weighted gene co-expression network analysis (WGCNA).

Applications

Unspecified application

Species

Unspecified reactive species

Jing Chen,Shi-Fu Luo,Xin Yuan,Mi Wang,Hai-Jie Yu,Zheng Zhang,Yong-Yu Yang

Cell reports 32:108150 PubMed32937134

2020

Astrocyte- and Neuron-Derived CXCL1 Drives Neutrophil Transmigration and Blood-Brain Barrier Permeability in Viral Encephalitis.

Applications

Unspecified application

Species

Unspecified reactive species

Benedict D Michael,Laura Bricio-Moreno,Elizabeth W Sorensen,Yoshishige Miyabe,Jeffrey Lian,Tom Solomon,Evelyn A Kurt-Jones,Andrew D Luster

International journal of molecular sciences 21: PubMed32708100

2020

Suppression of Ocular Vascular Inflammation through Peptide-Mediated Activation of Angiopoietin-Tie2 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Adam C Mirando,Raquel Lima E Silva,Zenny Chu,Peter A Campochiaro,Niranjan B Pandey,Aleksander S Popel

Parasites & vectors 13:254 PubMed32410640

2020

Xiaochaihu decorction relieves liver fibrosis caused by Schistosoma japonicum infection via the HSP47/TGF-β pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuzheng Huang,Jin Lu,Yongliang Xu,Chunrong Xiong,Deshen Tong,Nannan Hu,Haitao Yang

Molecular therapy : the journal of the American Society of Gene Therapy 28:1442-1454 PubMed32278382

2020

A Highly Efficacious PS Gene Editing System Corrects Metabolic and Neurological Complications of Mucopolysaccharidosis Type I.

Applications

Unspecified application

Species

Unspecified reactive species

Li Ou,Michael J Przybilla,Ozan Ahlat,Sarah Kim,Paula Overn,Jeanine Jarnes,M Gerard O'Sullivan,Chester B Whitley

Environmental pollution (Barking, Essex : 1987) 261:114183 PubMed32105967

2020

Prebiotic inulin consumption reduces dioxin-like PCB 126-mediated hepatotoxicity and gut dysbiosis in hyperlipidemic Ldlr deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jessie B Hoffman,Michael C Petriello,Andrew J Morris,M Abdul Mottaleb,Yipeng Sui,Changcheng Zhou,Pan Deng,Chunyan Wang,Bernhard Hennig

FASEB bioAdvances 2:219-233 PubMed32259049

2020

Hepatocyte growth factor is upregulated in ischemic retina and contributes to retinal vascular leakage and neovascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria E Lorenc,Raquel Lima E Silva,Sean F Hackett,Seth D Fortmann,Yuanyuan Liu,Peter A Campochiaro

Diabetes, metabolic syndrome and obesity : targets 13:313-323 PubMed32104028

2020

Caspase-3 Promotes Diabetic Kidney Disease Through Gasdermin E-Mediated Progression to Secondary Necrosis During Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Si Wen,Zhao-Hua Wang,Cong-Xiao Zhang,Ying Yang,Qiu-Ling Fan

Nature communications 11:694 PubMed32019921

2020

Sustained treatment of retinal vascular diseases with self-aggregating sunitinib microparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroki Tsujinaka,Jie Fu,Jikui Shen,Yun Yu,Zibran Hafiz,Joshua Kays,David McKenzie,Delia Cardona,David Culp,Ward Peterson,Brian C Gilger,Christopher S Crean,Jin-Zhong Zhang,Yogita Kanan,Weiling Yu,Jeffrey L Cleland,Ming Yang,Justin Hanes,Peter A Campochiaro

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Mouse Albumin ELISA Kit

Competitive ELISA - Mouse Albumin ELISA Kit (AB108791)

Competitive ELISA - Mouse Albumin ELISA Kit (AB108791)

Competitive ELISA - Mouse Albumin ELISA Kit (AB108791)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

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