Mouse Albumin ELISA Kit

Product details

Mouse Albumin ELISA kit (ab108792) is a sandwich ELISA kit designed for the quantitative measurement of mouse Albumin levels in plasma, serum, urine, cell culture supernatant, cell lysate and tissue samples.

An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Albumin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Mouse Albumin ELISA Kit ab108792 is a competitive ELISA to measure mouse albumin in urine and cell culture supernatants with a sensitivity of > 0.45 ng/mL.

How the assay works

An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Albumin captured in plate.

Assay specificity

The assay has no reactivity with canine, bovine, equine, monkey, human, swine, rabbit albumin.
the reactivity with rat albumin is <10%

Mouse Albumin ELISA Kit ab108792 protocol summary

1. Add 25 µl of Standard or Sample and 25 µl of Biotinylated Protein per well. Incubate 1 hour.
2. Wash, then add 50 µl of SP Conjugate per well. Incubate 30 minutes.
3. Wash, then add 50 µl of Chromogen Substrate per well. Incubate 20 minutes.
4. Add 50 µl of Stop Solution per well. Read at 450 nm immediately.

How other researchers are using Mouse Albumin ELISA kit ab108792

Mouse Albumin ELISA kit ab108792 has been used to measure the Albumin Creatinin Ratio (ACR) in mice with polycystic kidney disease⁽¹⁾ and to assess hepatocyte derived organoids ⁽²⁾

References:

⁽¹⁾MS Tham etal., 2024.PMID:38191531,
⁽²⁾J Liang et al., 2023, PMID:37907970

Get results in 90 minutes our SimpleStep ELISA® kit:

- Mouse albumin ELISA kit ab207620

Mouse Albumin ELISA kit ab109792 is commonly used by researchers to measure liver or kidney function.

Liver function ELISA assays include Mouse ALT ELISA kit ab282882 and Mouse AST ELISA Kit ab263882

These ELISA kits are also available for Rat:

- Rat Albumin ELISA kit ab235642
- Rat ALT ELISA kit abab234579
- Rat AST ELISA kit ab263883

Kidney function marker assays

- Creatinine Assay Kit ab65340 – suitable for use as a serum creatinine assay and a urine creatinine assay
- Albumin (BCG) Assay Kit (Colorimetric) ab235628 – suitable for use with serum
- Urea Assay Kit ab83362 – suitable for use with serum/plasma and urine, and used as a blood urea nitrogen assay.