Mouse Albumin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Albumin with a sensitivity of 25 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 15 citations
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- Nature communications 14:62462023Decreased liver B vitamin-related enzymes as a metabolic hallmark of cancer cachexia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYasushi Kojima,Emi Mishiro-Sato,Teruaki Fujishita,Kiyotoshi Satoh,Rie Kajino-Sakamoto,Isao Oze,Kazuki Nozawa,Yukiya Narita,Takatsugu Ogata,Keitaro Matsuo,Kei Muro,Makoto Mark Taketo,Tomoyoshi Soga,Masahiro AokiPubMed 37803016
- Journal of pineal research 75:e129132023Orchestrated feedback regulation between melatonin and sex hormones involving GPER1-PKA-CREB signaling in the placenta.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuan Shao,Yun Yang,Yanlei Liu,Yongqing Wang,Yangyu Zhao,Xin Yu,Juan Liu,Yu-Xia Li,Yan-Ling WangPubMed 37746893
- Nature communications 13:75762022The role of the tryptophan-NAD + pathway in a mouse model of severe malnutrition induced liver dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuanlan Hu,Catriona Ling,Lijun Chi,Mehakpreet K Thind,Samuel Furse,Albert Koulman,Jonathan R Swann,Dorothy Lee,Marjolein M Calon,Celine Bourdon,Christian J Versloot,Barbara M Bakker,Gerard Bryan Gonzales,Peter K Kim,Robert H J BandsmaPubMed 36481684
- Scientific reports 12:140792022Efficient engraftment and viral transduction of human hepatocytes in an FRG rat liver humanization model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarisa Carbonaro,Jeffrey Lee,Evangelos Pefanis,Mathieu Desclaux,Kehui Wang,Alexander Pennington,Hui Huang,Alejo Mujica,Jose Rojas,Roxanne Ally,Daniel Kennedy,Michael Brown,Vitaliy Rogulin,Sven Moller-Tank,Leah Sabin,Brian Zambrowicz,Gavin Thurston,Zhe LiPubMed 35982097
- Nature communications 13:48042022Mannose ameliorates experimental colitis by protecting intestinal barrier integrity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLijun Dong,Jingwen Xie,Youyi Wang,Honglian Jiang,Kai Chen,Dantong Li,Jing Wang,Yunzhi Liu,Jia He,Jia Zhou,Liyun Zhang,Xiao Lu,Xiaoming Zou,Xiang-Yang Wang,Qingqing Wang,Zhengliang Chen,Daming ZuoPubMed 35974017
- Immunity, inflammation and disease 10:e6242022Effects of Astragalus membranaceus on systemic lupus erythematosus in a mouse model of pregnancy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong-Qing Chen,Na Zhang,Cai-Xia Li,Hong-Xia ZhangPubMed 35634952
- Fluids and barriers of the CNS 19:312022A novel chronic dural port platform for continuous collection of cerebrospinal fluid and intrathecal drug delivery in free-moving mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTsuneo Nakajima,Shuko Takeda,Yuki Ito,Akane Oyama,Yoichi Takami,Yasushi Takeya,Koichi Yamamoto,Ken Sugimoto,Hideo Shimizu,Munehisa Shimamura,Hiromi Rakugi,Ryuichi MorishitaPubMed 35505336
- Circulation research 130:1662-16812022Perivascular Fibrosis Is Mediated by a KLF10-IL-9 Signaling Axis in CD4+ T Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRulin Zhuang,Jingshu Chen,Henry S Cheng,Carmel Assa,Anurag Jamaiyar,Arvind K Pandey,Daniel Pérez-Cremades,Bofang Zhang,Aspasia Tzani,Akm Khyrul Wara,Jorge Plutzky,Victor Barrera,Preetida Bhetariya,Richard N Mitchell,Zhongmin Liu,Mark W FeinbergPubMed 35440172
- International journal of molecular sciences 23:2022Fecal Microbiota Transplant in a Pre-Clinical Model of Type 2 Diabetes Mellitus, Obesity and Diabetic Kidney Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRosana M C Bastos,Antônio Simplício-Filho,Christian Sávio-Silva,Luiz Felipe V Oliveira,Giuliano N F Cruz,Eliza H Sousa,Irene L Noronha,Cristóvão L P Mangueira,Heloísa Quaglierini-Ribeiro,Gleice R Josefi-Rocha,Érika B RangelPubMed 35409202
- Frontiers in pharmacology 12:7773952022Lipidomic Analysis Reveals the Protection Mechanism of GLP-1 Analogue Dulaglutide on High-Fat Diet-Induced Chronic Kidney Disease in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartin Ho Yin Yeung,Ka Long Leung,Lai Yuen Choi,Jung Sun Yoo,Susan Yung,Pui-Kin So,Chi-Ming WongPubMed 35299724
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 25 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (By similarity). Does not prevent iron uptake by the bacterial siderophore aerobactin (By similarity).
Alternative names
Alb-1, Alb1, Alb, Albumin
What's included?
Recommended products
Mouse Albumin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Albumin with a sensitivity of 25 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 15 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 25 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 5 | C.V. 2.7 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 9.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 98 | Range 96 - 99 % |
Sample type EDTA Plasma | Average % = 94 | Range 84 - 101 % |
Sample type Urine | Average % = 87 | Range 73 - 107 % |
Sample type Heparin Plasma | Average % = 107 | Range 104 - 108 % |
Sample type Citrate plasma | Average % = 91 | Range 84 - 99 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse Albumin ELISA Kit ab207620 is a rapid single-wash 90-min ELISA kit to measure Mouse Albumin in urine, serum and plasma. This SimpleStep ELISA® sensitivity is 25 pg/mL.
How the assay works
Mouse Albumin SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native mouse Albumin protein serum, plasma and urine. Tissue and cell extract samples have not been tested with this kit.
Human serum albumin was prepared at 50 ng/mL and assayed for cross reactivity. No cross-reactivity was observed.
Mouse Albumin ELISA Kit ab207620 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Mouse Albumin ELISA kit ab207620
Mouse Albumin ELISA kit has been used in publications to study metabolic changes in cancer patients with cachexia(1) and effects of GLP-1 receptor agonists on kidney function (2).
References:
(1)Masahiro Aoki et al. 2023 PMID: 37803016, (2)Chi-Ming Wong et al. 2022 PMID: 35299724
Save your precious samples
Our 384-well Mouse Albumin ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Mouse Albumin Antibody Pair - BSA and Azide free Mouse Albumin Antibody Pair - BSA and Azide free ab241806
- Anti-Albumin antibody [EPR18741-27] - BSA and Azide free (Capture) Anti-Albumin antibody [EPR18741-27] - BSA and Azide free (Capture) ab242447
- Anti-Albumin antibody [EPR18741-67] - BSA and Azide free (Detector) Anti-Albumin antibody [EPR18741-67] - BSA and Azide free (Detector) ab242698
Related and recommended products
Mouse Albumin ELISA kit ab207620 is commonly used by researchers to measure liver or kidney function.
Liver function ELISA assays include Mouse ALT ELISA kit Mouse ALT ELISA Kit ab282882 and Mouse AST ELISA kit Mouse AST ELISA Kit (Aspartate Aminotransferase) ab263882
These ELISA kits are also available for Human and Rat:
- Human Albumin ELISA kit Human Albumin ELISA Kit ab179887
- Human ALT ELISA kit Human ALT ELISA Kit ab234578
- Human AST ELISA Kit Human AST ELISA Kit (Aspartate Aminotransferase) ab263881
- Rat Albumin ELISA kit Rat Albumin ELISA Kit ab235642
- Rat ALT ELISA kit abab234579
- Rat AST ELISA kit Rat AST ELISA Kit (Aspartate Aminotransferase) ab263883
Kidney function marker assays include:
- Creatinine Assay Kit Creatinine Assay Kit ab65340 - suitable for use as a serum creatinine assay and a urine creatinine assay.
- Albumin (BCG) Assay Kit (Colorimetric) Albumin (BCG) Assay Kit (Colorimetric) ab235628 – suitable for use with serum
- Urea Assay Kit Urea Assay Kit ab83362 – suitable for use with serum/plasma and urine, and used as a blood urea nitrogen assay.
Albumin (ALB), the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. It also serves as a major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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7 product images
Sandwich ELISA - Mouse Albumin ELISA Kit (ab207620)
Example of mouse Albumin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Albumin ELISA Kit (ab207620)
Example of mouse Albumin standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed
Sandwich ELISA - Mouse Albumin ELISA Kit (ab207620)
Example of mouse Albumin standard curve in Sample Diluent NS. The Serum Albumin standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Albumin ELISA Kit (ab207620)
Interpolated concentrations of mouse Albumin in mouse serum and plasma samples.
The concentrations of Serum Albumin were measured in duplicates, interpolated from the Serum Albumin standard curves and corrected for sample dilution. Undiluted is a 1:40,000,000 dilution for all samples. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serum Albumin concentration was determined to be 25 mg/mL in serum, 14 mg/mL in plasma (citrate) and 18 mg/mL in plasma (heparin) and 16 mg/mL in plasma (EDTA).
Sandwich ELISA - Mouse Albumin ELISA Kit (ab207620)
Interpolated concentrations of mouse Albumin in female mouse urine.
The concentrations of Serum Albumin were measured in duplicates, interpolated from the Serum Albumin standard curves and corrected for sample dilution. Undiluted is a 1:1,600 dilution for all samples. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serum Albumin concentration was determined to be 6.3 μg/mL in urine.
Sandwich ELISA - Mouse Albumin ELISA Kit (ab207620)
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Serum Albumin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Mouse Albumin ELISA Kit (ab207620)
Interpolated concentration of native Albumin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:10 million dilution mouse serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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