AB207620

Mouse Albumin ELISA Kit

Name: Mouse Albumin ELISA Kit (ab207620) | Abcam
Brand: Abcam Limited
SKU: AB207620
Price: 715 USD
Availability: InStock
Rating: 5 (1 reviews)

Recombinant
SimpleStep
What is this?

(1 Review)

(18 Publications)

Mouse Albumin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Albumin with a sensitivity of 25 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 15 citations

View Alternative Names

Alb-1, Alb1, Alb, Albumin

8 Images

ELISA - Mouse Albumin ELISA Kit (AB207620)

ELISA

Supplier Data

ELISA - Mouse Albumin ELISA Kit (AB207620)

sELISA

Supplier Data

Sandwich ELISA - Mouse Albumin ELISA Kit (AB207620)

Interpolated concentrations of mouse Albumin in mouse serum and plasma samples.

The concentrations of Serum Albumin were measured in duplicates, interpolated from the Serum Albumin standard curves and corrected for sample dilution. Undiluted is a 1 : 40,000,000 dilution for all samples. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serum Albumin concentration was determined to be 25 mg/mL in serum, 14 mg/mL in plasma (citrate) and 18 mg/mL in plasma (heparin) and 16 mg/mL in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Mouse Albumin ELISA Kit (AB207620)

Interpolated concentrations of mouse Albumin in female mouse urine.

The concentrations of Serum Albumin were measured in duplicates, interpolated from the Serum Albumin standard curves and corrected for sample dilution. Undiluted is a 1 : 1,600 dilution for all samples. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serum Albumin concentration was determined to be 6.3 μg/mL in urine.

sELISA

Lab

Sandwich ELISA - Mouse Albumin ELISA Kit (AB207620)

Example of mouse Albumin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Albumin ELISA Kit (AB207620)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native Serum Albumin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Mouse Albumin ELISA Kit (AB207620)

Example of mouse Albumin standard curve in Sample Diluent NS. The Serum Albumin standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Mouse Albumin ELISA Kit (AB207620)

Interpolated concentration of native Albumin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 10 million dilution mouse serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Mouse Albumin ELISA Kit (AB207620)

Example of mouse Albumin standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 25 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Albumin ELISA Kit ab207620 is a rapid single-wash 90-min ELISA kit to measure Mouse Albumin in urine, serum and plasma. This SimpleStep ELISA^® sensitivity is 25 pg/mL.

How the assay works

Mouse Albumin SimpleStep ELISA^® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

This kit recognizes both native mouse Albumin protein serum, plasma and urine. Tissue and cell extract samples have not been tested with this kit.
Human serum albumin was prepared at 50 ng/mL and assayed for cross reactivity. No cross-reactivity was observed.

Mouse Albumin ELISA Kit ab207620 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Mouse Albumin ELISA kit ab207620

Mouse Albumin ELISA kit has been used in publications to study metabolic changes in cancer patients with cachexia⁽¹⁾ and effects of GLP-1 receptor agonists on kidney function ⁽²⁾.

References:

⁽¹⁾Masahiro Aoki et al. 2023 PMID: 37803016, ⁽²⁾Chi-Ming Wong et al. 2022 PMID: 35299724

Save your precious samples

Our 384-well Mouse Albumin ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Mouse Albumin Antibody Pair - BSA and Azide free ab241806
- Anti-Albumin antibody [EPR18741-27] - BSA and Azide free (Capture) ab242447
- Anti-Albumin antibody [EPR18741-67] - BSA and Azide free (Detector) ab242698

Related and recommended products

Mouse Albumin ELISA kit ab207620 is commonly used by researchers to measure liver or kidney function.

Liver function ELISA assays include Mouse ALT ELISA kit ab282882 and Mouse AST ELISA kit ab263882

These ELISA kits are also available for Human and Rat:

- Human Albumin ELISA kit ab179887
- Human ALT ELISA kit ab234578
- Human AST ELISA Kit ab263881
- Rat Albumin ELISA kit ab235642
- Rat ALT ELISA kit ab234579
- Rat AST ELISA kit ab263883

Kidney function marker assays include:

- Creatinine Assay Kit ab65340 - suitable for use as a serum creatinine assay and a urine creatinine assay.
- Albumin (BCG) Assay Kit (Colorimetric) ab235628 – suitable for use with serum
- Urea Assay Kit ab83362 – suitable for use with serum/plasma and urine, and used as a blood urea nitrogen assay.

Albumin (ALB), the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. It also serves as a major zinc transporter in plasma, typically binds about 80% of all plasma zinc.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" } ]

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Recovery

[ { "sample": "Serum", "range": "96 - 99 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "84 - 101 %", "average": "= 94" }, { "sample": "Urine", "range": "73 - 107 %", "average": "= 87" }, { "sample": "Heparin Plasma", "range": "104 - 108 %", "average": "= 107" }, { "sample": "Citrate plasma", "range": "84 - 99 %", "average": "= 91" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab207620-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB207620-CMP06" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CP2", "number":"AB207620-CMP11" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB207620-CMP04" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB207620-CMP10" }, { "size":"2 x 1 Vial", "name":"Mouse Albumin Lyophilized Purified Protein", "number":"AB207620-CMP08" }, { "size":"1 x 600 µL", "name":"10X Mouse Albumin Capture Antibody", "number":"AB207620-CMP02" }, { "size":"1 x 600 µL", "name":"10X Mouse Albumin Detector Antibody", "number":"AB207620-CMP03" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB207620-CMP07" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB207620-CMP05" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB207620-CMP09" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab207620-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB207620-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CP2", "number":"AB207620-CMP11", "productcode":"" }, { "size":"2 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB207620-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Albumin Lyophilized Purified Protein", "number":"AB207620-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Albumin Capture Antibody", "number":"AB207620-CMP02", "productcode":"" }, { "size":"1 x 500 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB207620-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Albumin Detector Antibody", "number":"AB207620-CMP03", "productcode":"" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB207620-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB207620-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB207620-CMP12", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.

Biological function summary

Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.

Pathways

More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.

Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (By similarity). Does not prevent iron uptake by the bacterial siderophore aerobactin (By similarity).

See full target information Alb

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:6246 PubMed37803016

2023

Decreased liver B vitamin-related enzymes as a metabolic hallmark of cancer cachexia.

Applications

Unspecified application

Species

Unspecified reactive species

Yasushi Kojima,Emi Mishiro-Sato,Teruaki Fujishita,Kiyotoshi Satoh,Rie Kajino-Sakamoto,Isao Oze,Kazuki Nozawa,Yukiya Narita,Takatsugu Ogata,Keitaro Matsuo,Kei Muro,Makoto Mark Taketo,Tomoyoshi Soga,Masahiro Aoki

Journal of pineal research 75:e12913 PubMed37746893

2023

Orchestrated feedback regulation between melatonin and sex hormones involving GPER1-PKA-CREB signaling in the placenta.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Shao,Yun Yang,Yanlei Liu,Yongqing Wang,Yangyu Zhao,Xin Yu,Juan Liu,Yu-Xia Li,Yan-Ling Wang

Nature communications 13:7576 PubMed36481684

2022

The role of the tryptophan-NAD + pathway in a mouse model of severe malnutrition induced liver dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Guanlan Hu,Catriona Ling,Lijun Chi,Mehakpreet K Thind,Samuel Furse,Albert Koulman,Jonathan R Swann,Dorothy Lee,Marjolein M Calon,Celine Bourdon,Christian J Versloot,Barbara M Bakker,Gerard Bryan Gonzales,Peter K Kim,Robert H J Bandsma

Scientific reports 12:14079 PubMed35982097

2022

Efficient engraftment and viral transduction of human hepatocytes in an FRG rat liver humanization model.

Applications

Unspecified application

Species

Unspecified reactive species

Marisa Carbonaro,Jeffrey Lee,Evangelos Pefanis,Mathieu Desclaux,Kehui Wang,Alexander Pennington,Hui Huang,Alejo Mujica,Jose Rojas,Roxanne Ally,Daniel Kennedy,Michael Brown,Vitaliy Rogulin,Sven Moller-Tank,Leah Sabin,Brian Zambrowicz,Gavin Thurston,Zhe Li

Nature communications 13:4804 PubMed35974017

2022

Mannose ameliorates experimental colitis by protecting intestinal barrier integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Lijun Dong,Jingwen Xie,Youyi Wang,Honglian Jiang,Kai Chen,Dantong Li,Jing Wang,Yunzhi Liu,Jia He,Jia Zhou,Liyun Zhang,Xiao Lu,Xiaoming Zou,Xiang-Yang Wang,Qingqing Wang,Zhengliang Chen,Daming Zuo

Immunity, inflammation and disease 10:e624 PubMed35634952

2022

Effects of Astragalus membranaceus on systemic lupus erythematosus in a mouse model of pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Qing Chen,Na Zhang,Cai-Xia Li,Hong-Xia Zhang

Fluids and barriers of the CNS 19:31 PubMed35505336

2022

A novel chronic dural port platform for continuous collection of cerebrospinal fluid and intrathecal drug delivery in free-moving mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tsuneo Nakajima,Shuko Takeda,Yuki Ito,Akane Oyama,Yoichi Takami,Yasushi Takeya,Koichi Yamamoto,Ken Sugimoto,Hideo Shimizu,Munehisa Shimamura,Hiromi Rakugi,Ryuichi Morishita

Circulation research 130:1662-1681 PubMed35440172

2022

Perivascular Fibrosis Is Mediated by a KLF10-IL-9 Signaling Axis in CD4+ T Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rulin Zhuang,Jingshu Chen,Henry S Cheng,Carmel Assa,Anurag Jamaiyar,Arvind K Pandey,Daniel Pérez-Cremades,Bofang Zhang,Aspasia Tzani,Akm Khyrul Wara,Jorge Plutzky,Victor Barrera,Preetida Bhetariya,Richard N Mitchell,Zhongmin Liu,Mark W Feinberg

International journal of molecular sciences 23: PubMed35409202

2022

Fecal Microbiota Transplant in a Pre-Clinical Model of Type 2 Diabetes Mellitus, Obesity and Diabetic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Rosana M C Bastos,Antônio Simplício-Filho,Christian Sávio-Silva,Luiz Felipe V Oliveira,Giuliano N F Cruz,Eliza H Sousa,Irene L Noronha,Cristóvão L P Mangueira,Heloísa Quaglierini-Ribeiro,Gleice R Josefi-Rocha,Érika B Rangel

Frontiers in pharmacology 12:777395 PubMed35299724

2022

Lipidomic Analysis Reveals the Protection Mechanism of GLP-1 Analogue Dulaglutide on High-Fat Diet-Induced Chronic Kidney Disease in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Ho Yin Yeung,Ka Long Leung,Lai Yuen Choi,Jung Sun Yoo,Susan Yung,Pui-Kin So,Chi-Ming Wong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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