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AB285274

Mouse Alkaline Phosphatase ELISA Kit

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(1 Publication)

Mouse Alkaline Phosphatase ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse Alkaline Phosphatase in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Akp-3, Akp3, Iap, Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase, Alkaline phosphatase 3

1 Images
Sandwich ELISA - Mouse Alkaline Phosphatase ELISA Kit (AB285274)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Alkaline Phosphatase ELISA Kit (AB285274)

ALP Standard Curve.

Standard curve anaylsis. For reference use only.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 18.75 pg/mL

Range

31.25 - 2000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Mouse ALP ELISA kit (ab285274, E4573) is a sandwich ELISA assay for the quantitative measurement of ALP in mouse serum, plasma and cell culture supernatants. The density of color is proportional to the amount of ALP captured from the samples.
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Precision

[ { "reproducibilityType": "Intra", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 8" }, { "reproducibilityType": "Inter", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alkaline Phosphatase also known as ALP or ALKPHOS is an enzyme that removes phosphate groups from various molecules like nucleotides and proteins. This enzymatic activity requires alkaline conditions with a pH optimum around 8–10. ALP is anchored to the cell membrane and exists in several isoforms each with a mass around 57-70 kDa. Different tissues express specific isoforms such as bone liver kidney and placenta. Osteoblasts for example show high levels of alkaline phosphatase expression often detected by techniques like ALP staining or alkaline phosphatase staining.
Biological function summary

Alkaline phosphatase plays important roles in dephosphorylation processes. Particularly in bone it is associated with osteoblast activity where it is important for bone mineralization. The enzyme is not known to be part of a large protein complex but its activity aids in the conversion of inorganic pyrophosphate into phosphate which promotes the deposition of calcium phosphate in bone. ALP staining and ALP staining osteoblast can be used in laboratory settings for studying the mineralization and function of osteoblasts.

Pathways

Alkaline phosphatase is key in the regulation of the phosphate group levels within the organism particularly in the bone mineralization process. It participates in the vitamin D metabolic pathway which influences calcium and phosphorus homeostasis. Its function has a dynamic relationship with proteins like NPP1 which regulates levels of inorganic pyrophosphate acting counter to alkaline phosphatase's phosphate-producing activity.

Defects in alkaline phosphatase activity are linked to conditions like hypophosphatasia and hyperphosphatasia where mineralization processes are disrupted. In hypophosphatasia decreased ALP activity leads to improper bone formation due to accumulation of inorganic pyrophosphate. This interaction is notable alongside proteins such as tissular nonspecific alkaline phosphatase. On the other hand elevated ALP levels often indicate liver or bone disease pathology where increased enzyme levels reflect altered tissue turnover.
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Product protocols

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Target data

Alkaline phosphatase that can hydrolyze various phosphate compounds.
See full target information Iap
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 28:52 PubMed35508992

2022

Resveratrol ameliorates liver fibrosis induced by nonpathogenic Staphylococcus in BALB/c mice through inhibiting its growth.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqin Li,Jianxia Dong,Meng Wang,Jingya Yan,Yushu Hu,Yang Liu,Yajie Pan,Hua Li
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