AB264605

Mouse alpha 1 acid Glycoprotein ELISA Kit

Recombinant
SimpleStep
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Mouse alpha 1 acid Glycoprotein ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse alpha 1 acid Glycoprotein with a sensitivity of 111.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Agp1, Orm-1, Orm1, Alpha-1-acid glycoprotein 1, AGP 1, Orosomucoid-1, OMD 1

5 Images

ELISA - Mouse alpha 1 acid Glycoprotein ELISA Kit (AB264605)

ELISA

Supplier Data

ELISA - Mouse alpha 1 acid Glycoprotein ELISA Kit (AB264605)

sELISA

Supplier Data

Sandwich ELISA - Mouse alpha 1 acid Glycoprotein ELISA Kit (AB264605)

Interpolated concentrations of native alpha 1 acid Glycoprotein in mouse serum and plasma samples.

The concentrations of alpha 1 acid Glycoprotein were measured in duplicates, interpolated from the alpha 1 acid Glycoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 2,000, plasma (citrate) 1 : 8,000, plasma (heparin) 1 : 4,000, and plasma (EDTA) 1 : 2,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha 1 acid Glycoprotein concentration was determined to be 16.84 μg/mL in serum, 47.40 μg/mL in plasma (citrate), 24.08 μg/ml in plasma (heparin), and 17.47 μg/ml in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Mouse alpha 1 acid Glycoprotein ELISA Kit (AB264605)

Interpolated concentrations of native alpha 1 acid Glycoprotein in mouse liver tissue culture supernatant samples.

The concentrations of alpha 1 acid Glycoprotein were measured in duplicates, interpolated from the alpha 1 acid Glycoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows : mouse liver supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha 1 acid Glycoprotein concentration was determined to be 10.66 ng/mL in liver supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse alpha 1 acid Glycoprotein ELISA Kit (AB264605)

Example of mouse alpha 1 acid Glycoprotein standard curve in Sample Diluent NS.

The alpha 1 acid Glycoprotein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse alpha 1 acid Glycoprotein ELISA Kit (AB264605)

Interpolated concentrations of native alpha 1 acid Glycoprotein in male mouse urine samples.

The concentrations of alpha 1 acid Glycoprotein were measured in duplicates, interpolated from the alpha 1 acid Glycoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows : male mouse urine 1 : 256. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha 1 acid Glycoprotein concentration was determined to be 1.94 μg/mL in male mouse urine.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 111.4 pg/mL

Range

156.25 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse alpha 1 acid Glycoprotein ELISA Kit ab264605 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse alpha 1 acid Glycoprotein in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, urine. This SimpleStep sensitivity is 111.4 pg/mL.

How the assay works

Mouse alpha 1 acid Glycoprotein SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse alpha 1 acid Glycoprotein ELISA Kit ab264605 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Alpha 1 acid glycoprotein 1 (ORM1) is a plasma protein involved in acute inflammation. Alpha 1 acid glycoprotein is a transport protein for various cellular ligands and synthetic drugs. Alpha 1 acid glycoprotein is expressed by the liver and is induced by glucocorticoids, IL-1 and IL-6.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "98 - 112 %", "average": "= 103" }, { "sample": "Serum", "range": "99 - 108 %", "average": "= 104" }, { "sample": "EDTA Plasma", "range": "108 - 114 %", "average": "= 111" }, { "sample": "Heparin Plasma", "range": "100 - 116 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "94 - 112 %", "average": "= 103" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab264605-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB264605-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264605-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB264605-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB264605-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB264605-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB264605-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse alpha 1 acid Glycoprotein Lyophilized Recombinant Protein", "number":"AB264605-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse alpha 1 acid Glycoprotein Capture Antibody", "number":"AB264605-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB264605-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse alpha 1 acid Glycoprotein Detector Antibody", "number":"AB264605-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Acid Glycoprotein also known as AGP or Alpha 1 Glycoprotein is a plasma protein with a molecular weight of approximately 41 to 43 kDa. This protein circulates primarily in the bloodstream and is produced mainly by the liver. AGP is known for its highly glycosylated structure carrying several branched oligosaccharide chains. It exhibits a significant capacity to bind various endogenous and exogenous lipophilic substances influencing their transport and distribution in the body.

Biological function summary

AGP plays a role in modulating the immune response and maintaining homeostasis. As an acute-phase protein its levels rise significantly during inflammation infection and trauma where it acts to modulate the immune system. AGP does not exist as part of a larger protein complex but interacts with plasma lipoproteins and other glycoproteins aiding in its function of maintaining vascular integrity during acute-phase reactions.

Pathways

Alpha 1 Glycoprotein serves as an important component in the inflammatory response and drug-binding pathways. In the inflammatory pathway AGP interacts with cytokines and acute-phase reactants adjusting the body’s inflammatory processes. It is also involved in the binding and distribution of drugs interacting with albumin and other transport proteins to influence the pharmacokinetics of many medications.

AGP levels change in response to conditions like rheumatoid arthritis and liver diseases. In rheumatoid arthritis its increased levels correlate with disease activity and inflammation. In liver diseases the production and plasma concentration of AGP can reflect hepatic synthesis capability. AGP's interaction with cytokines in these conditions often links it to elevated levels of other acute-phase proteins like C-reactive protein reflecting its participation in the pathophysiological processes of inflammation.

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Product protocols

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Target data

Functions as a transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain (By similarity). Appears to function in modulating the activity of the immune system during the acute-phase reaction.

See full target information Orm1

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse alpha 1 acid Glycoprotein ELISA Kit

ELISA - Mouse alpha 1 acid Glycoprotein ELISA Kit (AB264605)

Sandwich ELISA - Mouse alpha 1 acid Glycoprotein ELISA Kit (AB264605)

Sandwich ELISA - Mouse alpha 1 acid Glycoprotein ELISA Kit (AB264605)

Sandwich ELISA - Mouse alpha 1 acid Glycoprotein ELISA Kit (AB264605)

Sandwich ELISA - Mouse alpha 1 acid Glycoprotein ELISA Kit (AB264605)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Secondary Antibodies

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Product promise