JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB205088

Mouse Alpha 1 Antitrypsin ELISA Kit

Be the first to review this product! Submit a review

|

(7 Publications)

Mouse Alpha 1 Antitrypsin ELISA Kit is a sandwich ELISA designed to quantify Mouse Alpha 1 Antitrypsin with a sensitivity of 1.492 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 6.25 - 200 ng/mL

View Alternative Names

Dom1, Spi1-1, Serpina1a, Alpha-1-antitrypsin 1-1, AAT, Alpha-1 protease inhibitor 1, Alpha-1-antiproteinase, Serine protease inhibitor 1-1, Serine protease inhibitor A1a, Serpin A1a

1 Images
ELISA - Mouse Alpha 1 Antitrypsin ELISA Kit (AB205088)
  • ELISA

Supplier Data

ELISA - Mouse Alpha 1 Antitrypsin ELISA Kit (AB205088)

Representative standard curve using ab205088 Mouse Alpha 1 Antitrypsin ELISA Kit.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Urine

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 1.492 ng/mL

Range

6.25 - 200 ng/mL

Assay time

2h 10m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Alpha 1 Antitrypsin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Alpha 1 Antitrypsin protein in mouse biological samples.

In this assay the A1AT present in samples reacts with the anti-A1AT antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-A1AT antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound A1AT . Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of A1AT in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of A1AT in the test sample. The quantity of A1AT in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": null, "average": ">= 85" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab205088-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"5X Diluent Concentrate", "number":"AB205088-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB205088-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Mouse A1AT Calibrator (lyophilized)", "number":"AB205088-CMP05", "productcode":"" }, { "size":"1 x 150 µL", "name":"100X HRP Conjugated Enzyme Antibody", "number":"AB205088-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB205088-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB205088-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Mouse A1AT ELISA Microplate", "number":"AB205088-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Antitrypsin also known as A1AT or alpha-1 proteinase inhibitor is a serine protease inhibitor with a molecular mass of about 52 kDa. This protein mainly expresses in the liver and found in high concentrations in the blood plasma. A1AT protects tissues from enzymes of inflammatory cells especially neutrophil elastase. Its expression level is regulated by the liver making it a significant player in maintaining tissue integrity.
Biological function summary

A1AT regulates protease activity by forming complexes with target enzymes. It specifically inhibits neutrophil elastase a powerful enzyme capable of degrading elastin an important component of connective tissues. A1AT prevents excessive tissue damage during inflammation by maintaining a balance in protease activity within connective tissues across various organs.

Pathways

Alpha 1 Antitrypsin functions within the proteolytic pathways involved in inflammatory response and tissue remodeling. A1AT is closely related to neutrophil elastase in these processes. It interacts with other protease inhibitors like alpha 2-macroglobulin reinforcing its protective role against enzymatic activity that can lead to tissue destruction under pathophysiological conditions.

Alpha 1 Antitrypsin deficiency is a genetic condition that can cause chronic obstructive pulmonary disease (COPD) and liver cirrhosis. Deficient A1AT levels result in unregulated elastase activity leading to lung tissue damage and impaired liver function. Other proteins such as MMP-9 collaborate with elastase in exacerbating tissue damage illustrating how insufficient A1AT can significantly contribute to disease development.
style
left-column

Product protocols

style
left-column

Target data

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin.
See full target information Serpina1a
style
left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of ovarian research 18:109 PubMed40420300

2025

a1-antitrypsin, a new biomarker of polycystic ovary syndrome by changing its expression and rhythm.

Applications

Unspecified application

Species

Unspecified reactive species

Na Li,Beilei Shen,Wei Cao,RouRou Chen,Rongbo He,Li Qian,Lin Xu,Yu Liu

Nature communications 16:545 PubMed39788946

2025

The NE/AAT/CBG axis regulates adipose tissue glucocorticoid exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Luke D Boyle,Allende Miguelez-Crespo,Mhairi Paul,Elisa Villalobos,Julia N C Toews,Lisa Ivatt,Boglarka Nagy,Marisa Magennis,Natalie Z M Homer,Ruth Andrew,Victor Viau,Geoffrey L Hammond,Roland H Stimson,Brian R Walker,Mark Nixon

Nature communications 15:9585 PubMed39532838

2024

Hepatic SerpinA1 improves energy and glucose metabolism through regulation of preadipocyte proliferation and UCP1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Shota Okagawa,Masaji Sakaguchi,Yuma Okubo,Yuri Takekuma,Motoyuki Igata,Tatsuya Kondo,Naoki Takeda,Kimi Araki,Bruna Brasil Brandao,Wei-Jun Qian,Yu-Hua Tseng,Rohit N Kulkarni,Naoto Kubota,C Ronald Kahn,Eiichi Araki

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed34244428

2021

Hypoimmune induced pluripotent stem cell-derived cell therapeutics treat cardiovascular and pulmonary diseases in immunocompetent allogeneic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tobias Deuse,Grigol Tediashvili,Xiaomeng Hu,Alessia Gravina,Annika Tamenang,Dong Wang,Andrew Connolly,Christian Mueller,Beñat Mallavia,Mark R Looney,Malik Alawi,Lewis L Lanier,Sonja Schrepfer

PloS one 16:e0246040 PubMed33539438

2021

Knockdown of Alpha-1 Antitrypsin with antisense oligonucleotide does not exacerbate smoke induced lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Kyle Stearns,Monica Goldklang,Rui Xiao,Tina Zelonina,Keith Blomenkamp,Jeffery Teckman,Jeanine M D'Armiento

American journal of physiology. Lung cellular and 316:L1165-L1173 PubMed31017014

2019

Sex-specific differences in emphysema using a murine antisense oligonucleotide model of α-1 antitrypsin deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Rashika Joshi,Mohit Ojha,Jana Lewis,Qiang Fan,Brett Monia,Shuling Guo,Brian M Varisco

Experimental and therapeutic medicine 14:4134-4140 PubMed29075339

2017

Berberine ameliorates non-alcoholic steatohepatitis in ApoE mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Yang,Xiao-Jie Ma,Ling Li,Lei Wang,Ying-Gi Chen,Jing Liu,Yan Luo,Zhen-Jie Zhuang,Wen-Jun Yang,Shu-Fei Zang,Jun-Ping Shi
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB207620

Mouse Albumin ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse Albumin ELISA Kit (AB207620)

  • 5
  • 1
Assay Kits

AB252356

Mouse Neutrophil Elastase ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse Neutrophil Elastase ELISA Kit (AB252356)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB230933

Mouse Thrombin ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse Thrombin ELISA Kit (AB230933)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com