Mouse alpha-1-antitrypsin ELISA Kit
- Recombinant
- SimpleStep
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(1 Publication)
Mouse alpha-1-antitrypsin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse alpha-1-antitrypsin with a sensitivity of 65 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Dom1, Spi1-1, Serpina1a, Alpha-1-antitrypsin 1-1, AAT, Alpha-1 protease inhibitor 1, Alpha-1-antiproteinase, Serine protease inhibitor 1-1, Serine protease inhibitor A1a, Serpin A1a
- sELISA
Supplier Data
Sandwich ELISA - Mouse alpha-1-antitrypsin ELISA Kit (AB267809)
Example of mouse alpha-1-antitrypsin standard curve in Sample Diluent NS.
The alpha-1-antitrypsin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse alpha-1-antitrypsin ELISA Kit (AB267809)
Interpolated concentrations of native alpha-1-antitrypsin in mouse serum, plasma (EDTA), plasma (citrate) and plasma (heparin) samples.
The concentrations of alpha-1-antitrypsin were measured in duplicates, interpolated from the alpha-1-antitrypsin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2.5%, plasma (EDTA) 1 : 133, plasma (citrate), and plasma (heparin) 3%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha-1-antitrypsin concentration was determined to be 210 μg/mL in serum, 416 μg/mL in plasma (EDTA), 149 μg/mL in plasma (citrate), and 221 μg/mL in plasma (heparin).
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Reactivity data
Product details
Mouse alpha-1-antitrypsin ELISA Kit (ab267809) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of alpha-1-antitrypsin protein in cell culture media, edta plasma, hep plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse alpha-1-antitrypsin with 65 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Mouse Alpha-1-antitrypsin is an inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
Precision
Recovery
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Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
A1AT regulates protease activity by forming complexes with target enzymes. It specifically inhibits neutrophil elastase a powerful enzyme capable of degrading elastin an important component of connective tissues. A1AT prevents excessive tissue damage during inflammation by maintaining a balance in protease activity within connective tissues across various organs.
Pathways
Alpha 1 Antitrypsin functions within the proteolytic pathways involved in inflammatory response and tissue remodeling. A1AT is closely related to neutrophil elastase in these processes. It interacts with other protease inhibitors like alpha 2-macroglobulin reinforcing its protective role against enzymatic activity that can lead to tissue destruction under pathophysiological conditions.
Product protocols
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Target data
Publications (1)
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Nature communications 13:4804 PubMed35974017
2022
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Product promise
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