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AB210969

Mouse alpha Fetoprotein ELISA Kit

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(5 Publications)

Mouse alpha Fetoprotein ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse alpha Fetoprotein with a sensitivity of 287 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, Afp

5 Images
ELISA - Mouse alpha Fetoprotein ELISA Kit (AB210969)
  • ELISA

Supplier Data

ELISA - Mouse alpha Fetoprotein ELISA Kit (AB210969)
Sandwich ELISA - Mouse alpha Fetoprotein ELISA Kit (AB210969)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse alpha Fetoprotein ELISA Kit (AB210969)

Interpolated concentrations of spike alpha Fetoprotein in mouse cell culture supernatant and urine samples.

The concentrations of alpha Fetoprotein were measured in duplicates, interpolated from the alpha Fetoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows : cell culture supernatant 50% and urine 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Mouse alpha Fetoprotein ELISA Kit (AB210969)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse alpha Fetoprotein ELISA Kit (AB210969)

Example of mouse alpha Fetoprotein standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse alpha Fetoprotein ELISA Kit (AB210969)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse alpha Fetoprotein ELISA Kit (AB210969)

Interpolated concentrations of native alpha Fetoprotein in mouse serum and plasma samples.

The concentrations of alpha Fetoprotein were measured in duplicates, interpolated from the alpha Fetoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (EDTA) 50%, plasma (heparin) 50%, and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha Fetoprotein concentration was determined to be 35.2 ng/mL in serum, 57.4 ng/mL in plasma (EDTA), 16.6 ng/mLin plasma (heparin), and 17.3 ng/mL in plasma (citrate).

Sandwich ELISA - Mouse alpha Fetoprotein ELISA Kit (AB210969)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse alpha Fetoprotein ELISA Kit (AB210969)

Interpolated concentrations of native alpha Fetoprotein in mouse serum samples.

The concentrations of alpha Fetoprotein were measured in duplicates, interpolated from the alpha Fetoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows : normal serum 50% and pregnant serum 1 : 200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha Fetoprotein concentration was determined to be 35.2 ng/mL in normal serum and 7226 ng/mL in pregnant mouse serum.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 287 pg/mL

Range

0.94 - 60 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse alpha Fetoprotein ELISA Kit ab210969 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse alpha Fetoprotein in cell culture supernatant, plasma, serum, urine. This SimpleStep sensitivity is 287 pg/mL.

How the assay works

Mouse alpha Fetoprotein SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse alpha Fetoprotein ELISA Kit ab210969 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Alpha fetoprotein is a secreted single chain glycoprotein expressed normally during fetal development as well as by liver pathologies, including cancer. It is a member of the albuminoid superfamily and may act as a carrier protein. Pregnant mice may have elevated alpha -fetoprotein levels derived from fetal circulation; male and female adults have low levels of circulating alpha -fetoprotein

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "98 - 101 %", "average": "= 99" }, { "sample": "Serum", "range": "117 - 123 %", "average": "= 120" }, { "sample": "Urine", "range": "96 - 101 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "108 - 113 %", "average": "= 110" }, { "sample": "EDTA Plasma", "range": "98 - 108 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "116 - 125 %", "average": "= 119" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab210969-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB210969-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB210969-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB210969-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB210969-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB210969-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB210969-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse alpha Fetoprotein Capture Antibody", "number":"AB210969-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse alpha Fetoprotein Lyophilized Recombinant Protein", "number":"AB210969-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse alpha Fetoprotein Detector Antibody", "number":"AB210969-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB210969-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-fetoprotein also known as alpha 1-fetoprotein or AFP is a glycoprotein composed of a single polypeptide chain with a molecular mass of approximately 69 kDa. AFP is primarily expressed in the fetal liver yolk sac and gastrointestinal tract during embryonic development. In adults the expression of AFP is limited and occurs mainly in the liver. The protein belongs to the albuminoid gene family and shares structural similarity with serum albumin contributing to its role in fetal serum transport.
Biological function summary

Alpha-fetoprotein functions as a fetal plasma protein with roles similar to serum albumin including the binding and transport of ligands such as bilirubin and fatty acids. It does not form part of any larger protein complex but is significant in modulating fetal immune tolerance. In the fetal environment AFP helps protect cells from maternal immune attacks by binding to estrogens and other growth-modulating factors. Its function during pregnancy is critical for maintaining homeostasis and ensuring the fetus's viability.

Pathways

Alpha 1-fetoprotein plays a role in several important biological pathways related to cellular growth and immune response modulation. One such pathway involves regulation of cell proliferation and differentiation in the developing liver. AFP interacts with cellular receptors that influence these processes. Additionally it plays a part in immune pathways interacting with elements that control immune overlooking to fetal antigens hence being linked to both hepatic and immune system proteins like albumin and estrogen receptors.

High levels of alpha-fetoprotein are linked to hepatocellular carcinoma and germ cell tumors such as yolk sac tumor. AFP serves as a tumor marker in these conditions aiding in both diagnosis and monitoring treatment response. In liver cancer AFP levels often correlate with tumor burden while in germ cell tumors it connects to proteins like beta-human chorionic gonadotropin (beta-hCG). Both markers are used together in clinical settings to provide insights into tumor progression and therapeutic efficacy.
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Product protocols

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Target data

Binds estrogens, fatty acids and metals.
See full target information Afp
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 13: PubMed40571481

2025

Targeting Annexin A2 to reactivate tumor-associated antigens presentation and relieve immune tolerance in liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxiao Tang,Jianxin Yang,Qicong Shen,Zelong Gao,Mengpu Wu,Chenghua Wu,Jicong Du,Min Li,Changquan Ling,Feng Lu,Yifeng Chai,Xin Dong,Jianxin Qian,Chenqi Li,Feng Xie,Zhenhong Guo,Hui Shen,Dongyao Wang

Archives of Razi Institute 79:593-600 PubMed39736964

2024

Unveiling of the Anti-Tumor Activity of Green Synthesized Zinc Nanoparticles against Ehrlich Solid Tumors in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

F Izadi,S Ramalakshmi

Nature communications 15:2581 PubMed38519484

2024

Cancer cell genetics shaping of the tumor microenvironment reveals myeloid cell-centric exploitable vulnerabilities in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Christel F A Ramirez,Daniel Taranto,Masami Ando-Kuri,Marnix H P de Groot,Efi Tsouri,Zhijie Huang,Daniel de Groot,Roelof J C Kluin,Daan J Kloosterman,Joanne Verheij,Jing Xu,Serena Vegna,Leila Akkari

Hepatology communications 6:2914-2924 PubMed35811443

2022

Loss of SLC46A1 decreases tumor iron content in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dongyao Wang,Huiwen Wu,Jianxin Yang,Min Li,Changquan Ling,Zelong Gao,Hongtao Lu,Hui Shen,Yuxiao Tang

Toxicology research 10:376-388 PubMed34141151

2021

Promotion effects of DEHP on hepatocellular carcinoma models: up-regulation of PD-L1 by activating the JAK2/STAT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Xu,Song Huang,Zi-Ming Xu,Ke Ji,Xiang Zhang,Wei-Ping Xu,Wei Wei
View all publications
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