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AB282865

Mouse Alpha-synuclein ELISA Kit

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(4 Publications)

Mouse Alpha-synuclein ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Alpha-synuclein with a sensitivity of 7.2 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Syn, Snca, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP

5 Images
ELISA - Mouse Alpha-synuclein ELISA Kit (AB282865)
  • ELISA

Supplier Data

ELISA - Mouse Alpha-synuclein ELISA Kit (AB282865)
Sandwich ELISA - Mouse Alpha-synuclein ELISA Kit (AB282865)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Alpha-synuclein ELISA Kit (AB282865)

Interpolated concentrations of native Alpha-synuclein in mouse serum, plasma (EDTA), plasma (heparin).

The concentrations of Alpha-synuclein were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 200, plasma (EDTA) 1 : 100, plasma (heparin) 1 : 200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 101 ng/mL in serum, 57 ng/mL in plasma (EDTA), and 157 ng/mL in and plasma (heparin).

Sandwich ELISA - Mouse Alpha-synuclein ELISA Kit (AB282865)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Alpha-synuclein ELISA Kit (AB282865)

Interpolated concentrations of native Alpha-synuclein in mouse brain extract.

based on a 0.6 μg/mL extract load. The concentrations of Alpha-synuclein were measured in duplicate and interpolated from the Alpha-synuclein standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Mouse Alpha-synuclein ELISA Kit (AB282865)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Alpha-synuclein ELISA Kit (AB282865)

Interpolated concentrations of native Alpha-synuclein in rat serum and plasmas.

The concentrations of Alpha-synuclein were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, plasma (EDTA) 12.5%, plasma (citrate) 25%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 4,055 pg/mL in serum, 3,930 pg/mL in plasma (EDTA), 2,132 pg/mL in plasma (citrate) and 4,658 pg/mL in and plasma (heparin).

Sandwich ELISA - Mouse Alpha-synuclein ELISA Kit (AB282865)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Alpha-synuclein ELISA Kit (AB282865)

Example of Mouse Alpha-synuclein standard curve in Sample Diluent NS.

The Alpha-synuclein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, EDTA Plasma, Heparin Plasma, Mouse brain extract, Serum, Tissue Extracts

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 7.2 pg/mL

Range

11.7 - 750 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Alpha-synuclein ELISA Kit ab282865 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Alpha-synuclein in cell culture media, EDTA plasma, heparin plasma, Mouse brain extract, serum, tissue extracts. This SimpleStep sensitivity is 7.2 pg/mL.

How the assay works

Mouse Alpha-synuclein SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Alpha-synuclein ELISA Kit ab282865 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Alpha-synuclein antibody [EPR23675-102] - BSA and Azide free (Capture) ab281053
- Anti-Alpha-synuclein antibody [EPR23675-54] - BSA and Azide free (Detector) ab281053

Alpha-synuclein is a 140- amino acid protein encoded by the SNCA gene, primarily found in neural tissue. It is a member of the synuclein family of proteins, including β-Synuclein and γ-Synuclein. Alpha-synuclein has been found concentrated in the presynaptic nerve terminals of neurons and has been found in the nucleus of neurons. Alpha-synuclein is a major component of Lewy Bodies. This protein appears to play a role in dopamine metabolism and vesicle trafficking.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Recovery

[ { "sample": "Citrate plasma", "range": "106 - 119 %", "average": "= 112" }, { "sample": "EDTA Plasma", "range": "96 - 104 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "98 - 102 %", "average": "= 101" }, { "sample": "Serum", "range": "102 - 118 %", "average": "= 110" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab282865-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB282865-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB282865-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB282865-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB282865-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB282865-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB282865-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB282865-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Alpha-synuclein Detector Antibody", "number":"AB282865-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB282865-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Alpha-synuclein Lyophilized Recombinant Protein", "number":"AB282865-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Alpha-synuclein Capture Antibody", "number":"AB282865-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.
Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.
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Product protocols

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Target data

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (By similarity). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (By similarity). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (By similarity). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed : 20798282, PubMed : 25246573). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (By similarity). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (By similarity).
See full target information Snca
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Brain communications 5:fcad285 PubMed37953845

2023

α-Synuclein expression in response to bacterial ligands and metabolites in gut enteroendocrine cells: an proof of concept study.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Hurley,Elisa Menozzi,Sofia Koletsi,Rachel Bates,Matthew E Gegg,Kai-Yin Chau,Hervé M Blottière,Jane Macnaughtan,Anthony H V Schapira

PloS one 18:e0291927 PubMed37733672

2023

Peptide immunization against the C-terminal of alpha-synuclein reduces locomotor activity in mice overexpressing alpha-synuclein.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Sung Chiu,Kuo-Jen Wu,Seong-Jin Yu,Kun-Lieh Wu,Yu-Syuan Wang,Jing Lin,Chia-Ying Chu,Shuchun Chen,Hsi Chen,Shu-Ching Hsu,Yun Wang,Yun-Hsiang Chen

The Journal of clinical investigation 133: PubMed37014703

2023

Accumulation of α-synuclein mediates podocyte injury in Fabry nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Fabian Braun,Ahmed Abed,Dominik Sellung,Manuel Rogg,Mathias Woidy,Oysten Eikrem,Nicola Wanner,Jessica Gambardella,Sandra D Laufer,Fabian Haas,Milagros N Wong,Bernhard Dumoulin,Paula Rischke,Anne Mühlig,Wiebke Sachs,Katharina von Cossel,Kristina Schulz,Nicole Muschol,Sören W Gersting,Ania C Muntau,Oliver Kretz,Oliver Hahn,Markus M Rinschen,Michael Mauer,Tillmann Bork,Florian Grahammer,Wei Liang,Thorsten Eierhoff,Winfried Römer,Arne Hansen,Catherine Meyer-Schwesinger,Guido Iaccarino,Camilla Tøndel,Hans-Peter Marti,Behzad Najafian,Victor G Puelles,Christoph Schell,Tobias B Huber

eLife 12: PubMed36626307

2023

Unconventional secretion of α-synuclein mediated by palmitoylated DNAJC5 oligomers.

Applications

Unspecified application

Species

Unspecified reactive species

Shenjie Wu,Nancy C Hernandez Villegas,Daniel W Sirkis,Iona Thomas-Wright,Richard Wade-Martins,Randy Schekman
View all publications
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB51253

Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]

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Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

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Secondary Antibodies

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB8227

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Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

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Reagents, Controls & Accessories

AB206978

Pre-Coated 96 Well Microplate (12 x 8 well strips) SimpleStep ELISA®

SimpleStep

ELISA - Pre-Coated 96 Well Microplate (12 x 8 well strips) SimpleStep ELISA® (AB206978)

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