AB282882

Mouse ALT ELISA Kit

Name: Mouse ALT ELISA Kit (ab282882) | Abcam
Brand: Abcam Limited
SKU: AB282882
Price: 725 USD
Availability: InStock
Rating: 5 (1 reviews)

Recombinant
SimpleStep
What is this?

(1 Review)

(37 Publications)

Mouse ALT ELISA Kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify mouse alanine aminotransferase (ALT) with a sensitivity of 4 pg/mL. The assay follows a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric Sandwich ELISA – 450 nm readout, compatible with any standard plate reader
- Different formats for different needs – 384-well format available for high-throughput applications
- Broad sample compatibility – works with serum, plasma
- Cited in over 15 publications

View Alternative Names

Gpt1, Gpt, Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT 1

5 Images

Sandwich ELISA - Mouse ALT ELISA Kit (AB282882)

sELISA

Lab

Sandwich ELISA - Mouse ALT ELISA Kit (AB282882)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

sELISA

Supplier Data

Sandwich ELISA - Mouse ALT ELISA Kit (AB282882)

Interpolated concentrations of native ALT in mouse serum, plasma samples.

The concentrations of ALT were measured in duplicates, interpolated from the ALT standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2.5%, plasma – citrate 5%, plasma -heparin 5% and plasma – EDTA 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ALT concentration was determined to be 67.60 ng/mL in serum, 30.36 ng/mL in plasma – citrate, 48.30 ng/mL in plasma – heparin and 38.86 ng/mL in plasma - EDTA.

sELISA

Supplier Data

Sandwich ELISA - Mouse ALT ELISA Kit (AB282882)

Example of mouse ALT standard curve in 1X Cell Extraction Buffer PTR.

The ALT standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Mouse ALT ELISA Kit (AB282882)

Interpolated concentration of native ALT was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 2.5% mouse serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Lab

Sandwich ELISA - Mouse ALT ELISA Kit (AB282882)

Example of mouse ALT standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 4 pg/mL

Range

46.88 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse ALT ELISA Kit ab282882 is a rapid single-wash 90-min ELISA kit to measure Mouse ALT in serum and plasma. This SimpleStep ELISA® sensitivity is 4 pg/mL.

How the assay works

Mouse ALT SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit is designed for the quantification of mouse ALT. The standard protein in this kit is full length mouse ALT. Native signal was detected in serum, plasma (citrate), plasma (EDTA), and plasma (heparin)sample types. Cell culture supernatant, saliva, urine, milk, CSF, cell extract, and tissue extract samples have not been tested with this kit.

Mouse ALT ELISA Kit ab282882 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Mouse ALT ELISA Kit ab282882

Mouse ALT ELISA Kit ab28288 has been used to study mitochondrial homeostatis in liver disease⁽¹⁾ and the effects of iron deficiency in cellular response to malaria ⁽²⁾.

References:

⁽¹⁾Ai-Lei Xu et al. 2024, PMID: 37902450,
⁽²⁾Hal Drakesmith et al. 2023, PMID: 37812650

Save your precious samples

Our 384-well Mouse ALT ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Mouse Alanine aminotransferase (ALT) Antibody Pair - BSA and Azide free ab253647
- Anti-Alanine Transaminase antibody [EPR21013-71] - BSA and Azide free (Capture) ab281207
- Anti-Alanine Transaminase antibody [EPR21013-202] - BSA and Azide free (Detector) ab281057

Related and recommended products

Mouse ALT ELISA kit ab234578 is commonly used by researchers to measure liver function.

Other ELISA kits to measure liver function include:

- Mouse AST ELISA kit ab263882
- Mouse Albumin ELISA kit ab282297
- Mouse Alkaline Phosphatase ELISA kit ab285274

ALT is an aminotransferase that plays a key role in metabolism. It forms pyruvate through the reversible transamination of alanine and 2-oxoglutarate; this reaction is pivotal for gluconeogenesis and amino acid degradation. It is part of a key blood panel diagnostic that is indicative of liver health.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.4" } ]

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Recovery

[ { "sample": "Serum", "range": "80 - 113 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "103 - 114 %", "average": "= 108" }, { "sample": "Heparin Plasma", "range": "85 - 95 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "86 - 113 %", "average": "= 95" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).

See full target information Gpt

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Publications (37)

Recent publications for all applications. Explore the full list and refine your search

Chemical biology & drug design 105:e70113 PubMed40317895

2025

Osthole Induces Hepatic Stellate Cell Ferroptosis to Alleviate Liver Fibrosis by Inhibiting the Y-Box Binding Protein 1-Wnt/β-Catenin Axis Through Downregulating Myocyte Enhancer Factor 2A.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Tong,Meng Liu,Liang Chen,Yi-He Lin,Qing Zheng

Nature communications 16:1806 PubMed39984450

2025

Targeted delivery of TGF-β mRNA to murine lung parenchyma using one-component ionizable amphiphilic Janus Dendrimers.

Applications

Unspecified application

Species

Unspecified reactive species

Jaclynn A Meshanni,Emily R Stevenson,Dapeng Zhang,Rachel Sun,Nathan A Ona,Erin K Reagan,Elena Abramova,Chang-Jiang Guo,Melissa Wilkinson,Ishana Baboo,Yuzi Yang,Liuyan Pan,Devendra S Maurya,Virgil Percec,Yongsheng Li,Andrew Gow,Drew Weissman,Elena N Atochina-Vasserman

Life metabolism 3:loae026 PubMed39873005

2025

Phytic acid-based nanomedicine against mTOR represses lipogenesis and immune response for metabolic dysfunction-associated steatohepatitis therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Fenghua Xu,Shoujie Zhao,Yejing Zhu,Jun Zhu,Lingyang Kong,Huichen Li,Shouzheng Ma,Bo Wang,Yongquan Qu,Zhimin Tian,Junlong Zhao,Lei Liu

BMJ open diabetes research & care 13: PubMed39842865

2025

Mechanism of TGIF1 on glycolipid metabolism disorders in mice with type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Fuyan Bai,Liping Zheng,Li Tao,Shikai Wang,Yuchen Li,Lijun Hou

International journal of molecular sciences 25: PubMed39596109

2024

Vine Tea Extract (VTE) Inhibits High-Fat Diet-Induced Adiposity: Evidence of VTE's Anti-Obesity Effects In Vitro and In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Wonchul Lim,Seongmin Choi,Jinhak Kim,Kwang-Soo Baek,Minkuk Park,Gakyung Lee,Tae-Gyu Lim

Nature metabolism 6:2281-2299 PubMed39578649

2024

Pathogenic role of acyl coenzyme A binding protein (ACBP) in Cushing's syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Pan,Ai-Ling Tian,Hui Chen,Yifan Xia,Allan Sauvat,Stephanie Moriceau,Flavia Lambertucci,Omar Motiño,Liwei Zhao,Peng Liu,Misha Mao,Sijing Li,Shuai Zhang,Adrien Joseph,Sylvère Durand,Fanny Aprahamian,Zeyu Luo,Yang Ou,Zhe Shen,Enfu Xue,Yuhong Pan,Vincent Carbonnier,Gautier Stoll,Sabrina Forveille,Marion Leduc,Giulia Cerrato,Alexandra Cerone,Maria Chiara Maiuri,Frederic Castinetti,Thierry Brue,Hongsheng Wang,Yuting Ma,Isabelle Martins,Oliver Kepp,Guido Kroemer

International journal of medical sciences 21:2770-2780 PubMed39512683

2024

LCN2 depletion aggravates sepsis-induced liver injury by regulating PTGS2-dependent ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Jiang,Zhi-Tian Jiang,Gang Zhao,Jing-Wen Cai,Jie Song,Jing Wang,Zhen Zhou,Qian Wang,Qi-Hua Ling

Nature biotechnology 43:1445-1457 PubMed39415058

2024

Lung and liver editing by lipid nanoparticle delivery of a stable CRISPR-Cas9 ribonucleoprotein.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Chen,Hesong Han,Sheng Zhao,Bryant Xu,Boyan Yin,Atip Lawanprasert,Marena Trinidad,Benjamin W Burgstone,Niren Murthy,Jennifer A Doudna

Translational oncology 50:102151 PubMed39388958

2024

Oncolytic vaccinia virus armed with 4-1BBL elicits potent and safe antitumor immunity and enhances the therapeutic efficiency of PD-1/PD-L1 blockade in a pancreatic cancer model.

Applications

Unspecified application

Species

Unspecified reactive species

Yushi Ju,Feiyu Dai,Yirong Wang,Zhenyu Ye,Yang Li,Songguang Ju,Yan Ge,Wei Chen

Heliyon 10:e38534 PubMed39391493

2024

Gut microbiota and their relationship with circulating adipokines in an acute hepatic encephalopathy mouse model induced by surgical bile duct ligation.

Applications

Unspecified application

Species

Unspecified reactive species

Bokyung Lee,Danbi Jo,Jihyun Park,Oh Yoen Kim,Juhyun Song

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Mouse ALT ELISA Kit

Sandwich ELISA - Mouse ALT ELISA Kit (AB282882)

Sandwich ELISA - Mouse ALT ELISA Kit (AB282882)

Sandwich ELISA - Mouse ALT ELISA Kit (AB282882)

Sandwich ELISA - Mouse ALT ELISA Kit (AB282882)

Sandwich ELISA - Mouse ALT ELISA Kit (AB282882)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (37)

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Secondary Antibodies

Assay Kits

Primary Antibodies

Product promise