Mouse ALT ELISA Kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify mouse alanine aminotransferase (ALT) with a sensitivity of 4 pg/mL. The assay follows a simple Mix-Wash-Read protocol with just one incubation and wash step.
- Colorimetric Sandwich ELISA – 450 nm readout, compatible with any standard plate reader
- Different formats for different needs – 384-well format available for high-throughput applications
- Broad sample compatibility – works with serum, plasma
- Cited in over 15 publications
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- Biomolecules & biomedicine 24:505-5192024Silencing METTL14 alleviates liver injury in non-alcoholic fatty liver disease by regulating mitochondrial homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Wang,Jun Yan,Long Han,Zi-Lin Zou,Ai-Lei XuPubMed 37902450
- PLoS pathogens 19:e10116792023Cellular iron governs the host response to malaria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah K Wideman,Joe N Frost,Felix C Richter,Caitlin Naylor,José M Lopes,Nicole Viveiros,Megan R Teh,Alexandra E Preston,Natasha White,Shamsideen Yusuf,Simon J Draper,Andrew E Armitage,Tiago L Duarte,Hal DrakesmithPubMed 37812650
- iScience 26:1072952023Roquin-1 resolves sepsis-associated acute liver injury by regulating inflammatory profiles via miRNA cargo in extracellular vesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Zheng,Wei Ling,Deming Zhu,Zhi Li,Yousheng Li,Haoming Zhou,Lianbao KongPubMed 37554446
- Journal of neuroinflammation 20:1302023Experimental hepatic encephalopathy causes early but sustained glial transcriptional changes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWouter Claeys,Lien Van Hoecke,Hannah Lernout,Clint De Nolf,Griet Van Imschoot,Elien Van Wonterghem,Daan Verhaege,Jonas Castelein,Anja Geerts,Christophe Van Steenkiste,Roosmarijn E VandenbrouckePubMed 37248507
- eLife 12:2023Gene and protein expression and metabolic flux analysis reveals metabolic scaling in liver ex vivo and in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNgozi D Akingbesote,Brooks P Leitner,Daniel G Jovin,Reina Desrouleaux,Dennis Owusu,Wanling Zhu,Zongyu Li,Michael N Pollak,Rachel J PerryPubMed 37219930
- Cells 12:2023Deletion of Inhibits Metastasis Formation of Liver Cancer in -Mutant Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMonserrat Gerardo-Ramírez,Vanessa Giam,Diana Becker,Marco Groth,Nils Hartmann,Helen Morrison,Helen L May-Simera,Markus P Radsak,Jens U Marquardt,Peter R Galle,Peter Herrlich,Beate K Straub,Monika HartmannPubMed 37174657
- Frontiers in oncology 13:11109162023Sec61 blockade therapy overrides resistance to proteasome inhibitors and immunomodulatory drugs in multiple myeloma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAntoine Domenger,Daniela Ricci,Véronique Mayau,Laleh Majlessi,Christophe Marcireau,Gilles Dadaglio,Caroline DemangelPubMed 36776330
- Cell reports 41:1118272022EGFR-phosphorylated GDH1 harmonizes with RSK2 to drive CREB activation and tumor metastasis in EGFR-activated lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiHoon Kang,Jaemoo Chun,Jung Seok Hwang,Chaoyun Pan,Jie Li,Austin C Boese,Isabelle Young,Courteney M Malin,Yibin Kang,Don L Gibbons,Gabriel Sica,Haian Fu,Suresh S Ramalingam,Lingtao Jin,Sumin KangPubMed 36516759
- The Journal of biological chemistry 299:1027282022rAAV-CRISPRa therapy corrects Rai1 haploinsufficiency and rescues selective disease features in Smith-Magenis syndrome mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao-Cheng Chang,Yu-Ju Lee,Sehrish Javed,Minza Haque,Ya-Ting Chang,Yu Cheng Lin,Cameron Oram,Wei-Hsiang HuangPubMed 36410433
- Frontiers in molecular biosciences 9:10088022022Exosomes derived from M2 macrophages induce angiogenesis to promote wound healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeifeng Lyu,Yuanqing Cai,Guangyang Zhang,Zhaopu Jing,Jialin Liang,Rupeng Zhang,Xiaoqian Dang,Chen ZhangPubMed 36304927
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 46.88 - 3000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 4 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).
Alternative names
Gpt1, Gpt, Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT 1
What's included?
Recommended products
Mouse ALT ELISA Kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify mouse alanine aminotransferase (ALT) with a sensitivity of 4 pg/mL. The assay follows a simple Mix-Wash-Read protocol with just one incubation and wash step.
- Colorimetric Sandwich ELISA – 450 nm readout, compatible with any standard plate reader
- Different formats for different needs – 384-well format available for high-throughput applications
- Broad sample compatibility – works with serum, plasma
- Cited in over 15 publications
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 46.88 - 3000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 4 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 6.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 95 | Range 80 - 113 % |
Sample type EDTA Plasma | Average % = 108 | Range 103 - 114 % |
Sample type Heparin Plasma | Average % = 91 | Range 85 - 95 % |
Sample type Citrate plasma | Average % = 95 | Range 86 - 113 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse ALT ELISA Kit ab282882 is a rapid single-wash 90-min ELISA kit to measure Mouse ALT in serum and plasma. This SimpleStep ELISA® sensitivity is 4 pg/mL.
How the assay works
Mouse ALT SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit is designed for the quantification of mouse ALT. The standard protein in this kit is full length mouse ALT. Native signal was detected in serum, plasma (citrate), plasma (EDTA), and plasma (heparin)sample types. Cell culture supernatant, saliva, urine, milk, CSF, cell extract, and tissue extract samples have not been tested with this kit.
Mouse ALT ELISA Kit ab282882 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Mouse ALT ELISA Kit ab282882
Mouse ALT ELISA Kit ab28288 has been used to study mitochondrial homeostatis in liver disease(1) and the effects of iron deficiency in cellular response to malaria (2).
References:
(1)Ai-Lei Xu et al. 2024, PMID: 37902450,
(2)Hal Drakesmith et al. 2023, PMID: 37812650
Save your precious samples
Our 384-well Mouse ALT ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Mouse Alanine aminotransferase (ALT) Antibody Pair - BSA and Azide free Mouse Alanine aminotransferase (ALT) Antibody Pair - BSA and Azide free ab253647
- Anti-Alanine Transaminase antibody [EPR21013-71] - BSA and Azide free (Capture) Anti-Alanine Transaminase antibody [EPR21013-71] - BSA and Azide free (Capture) ab281207
- Anti-Alanine Transaminase antibody [EPR21013-202] - BSA and Azide free (Detector) Anti-Alanine Transaminase antibody [EPR21013-202] - BSA and Azide free (Detector) ab281057
Related and recommended products
Mouse ALT ELISA kit Human ALT ELISA Kit ab234578 is commonly used by researchers to measure liver function.
Other ELISA kits to measure liver function include:
- Mouse AST ELISA kit Mouse AST ELISA Kit (Aspartate Aminotransferase) ab263882
- Mouse Albumin ELISA kit Mouse Prealbumin ELISA Kit ab282297
- Mouse Alkaline Phosphatase ELISA kit Mouse Alkaline Phosphatase ELISA Kit ab285274
ALT is an aminotransferase that plays a key role in metabolism. It forms pyruvate through the reversible transamination of alanine and 2-oxoglutarate; this reaction is pivotal for gluconeogenesis and amino acid degradation. It is part of a key blood panel diagnostic that is indicative of liver health.
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5 product images
Sandwich ELISA - Mouse ALT ELISA Kit (ab282882)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Sandwich ELISA - Mouse ALT ELISA Kit (ab282882)
Example of mouse ALT standard curve in 1X Cell Extraction Buffer PTR.
The ALT standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse ALT ELISA Kit (ab282882)
Interpolated concentrations of native ALT in mouse serum, plasma samples.
The concentrations of ALT were measured in duplicates, interpolated from the ALT standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2.5%, plasma – citrate 5%, plasma -heparin 5% and plasma – EDTA 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ALT concentration was determined to be 67.60 ng/mL in serum, 30.36 ng/mL in plasma – citrate, 48.30 ng/mL in plasma – heparin and 38.86 ng/mL in plasma - EDTA.
Sandwich ELISA - Mouse ALT ELISA Kit (ab282882)
Interpolated concentration of native ALT was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 2.5% mouse serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Mouse ALT ELISA Kit (ab282882)
Example of mouse ALT standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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