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AB320790

Mouse AMH ELISA Kit

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Mouse AMH ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse AMH with a sensitivity of 58 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Anti-Muellerian hormone, AMH, Muellerian-inhibiting factor, Muellerian-inhibiting substance, MIS, Amh

4 Images
Sandwich ELISA - Mouse AMH ELISA Kit (AB320790)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse AMH ELISA Kit (AB320790)

Interpolated concentrations of mouse AMH in mouse serum, plasma (citrate), plasma (EDTA), and plasma (heparin. Interpolated concentration of native AMH was measured in duplicate at different sample concentrations. Undiluted samples are 10% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse AMH ELISA Kit (AB320790)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse AMH ELISA Kit (AB320790)

Interpolated concentrations of mouse AMH in mouse serum in 96-well vs. 384-well plates. Interpolated concentration of native AMH was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 5% mouse serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse AMH ELISA Kit (AB320790)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse AMH ELISA Kit (AB320790)

Example of mouse AMH standard curve in Sample Diluent NS. Example of mouse AMH standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse AMH ELISA Kit (AB320790)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse AMH ELISA Kit (AB320790)

Example of mouse AMH standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Example of mouse AMH standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 58 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse AMH SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of AMH protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media. Quantitate Mouse AMH with 58 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" } ]
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Recovery

[ { "sample": "Serum", "range": "86 - 92 %", "average": "= 88" }, { "sample": "Citrate plasma", "range": "93 - 103 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "101 - 110 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "81 - 104 %", "average": "= 95" }, { "sample": "Cell culture media", "range": "95 - 104 %", "average": "= 98" }, { "sample": "Cell Lysate", "range": "84 - 105 %", "average": "= 97" }, { "sample": "Tissue Extracts", "range": "102 - 107 %", "average": "= 105" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

The anti-Muellerian hormone (AMH) plays an important role in several reproductive functions (PubMed : 11606457, PubMed : 11861535, PubMed : 1782869, PubMed : 9435237). Anti-Muellerian hormone binds and activates AMHR2, its specific type-II receptor, that heterodimerizes with type-I receptors (ACVR1 and BMPR1A) to regulate target gene expression through downstream SMAD protein signal transduction (By similarity). Produced and secreted by Sertoli cells of the male fetus, anti-Muellerian hormone induces Muellerian duct regression during male fetal sexual differentiation (PubMed : 1782869, PubMed : 9435237). In female, it is produced by granulosa cells of the preantral and small antral follicles and acts as a negative regulator of the primordial to primary follicle transition and decreases FSH sensitivity of growing follicles (PubMed : 11606457, PubMed : 11861535). Also plays a role in Leydig cell differentiation and function (PubMed : 1782869, PubMed : 9435237).. Plays an important role in several reproductive functions, including Muellerian duct regression during male fetal sexua,l differentiation and in the adult plays a role in Leydig cell differentiation and function (PubMed : 1782869, PubMed : 9435237). In female acts as a negative regulator of the primordial to primary follicle transition and decreases FSH sensitivity of growing follicles (PubMed : 11606457, PubMed : 11861535). Binds to its sole type II receptor, AMHR2 that recruits type I receptors ACVR1 and BMPR1A which subsequently activates the Smad pathway (By similarity).
See full target information Amh
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Product promise

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