Mouse Amphiregulin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse Amphiregulin in Mouse in Plasma, Cell culture supernatant, Serum samples.
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- Immunity 51:899-914.e72019Tissue-Specific Macrophage Responses to Remote Injury Impact the Outcome of Subsequent Local Immune Challenge.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFriedrich Felix Hoyer et. al.PubMed 31732166
- Genes to cells : devoted to molecular & cellular mechanisms 23:136-1452018Transgenic mice specifically expressing amphiregulin in white adipose tissue showed less adipose tissue mass.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Yang,Takahiro Kumoto,Takeshi Arima,Minako Nakamura,Yohei Sanada,Thanutchaporn Kumrungsee,Yusuke Sotomaru,Masayuki Shimada,Noriyuki YanakaPubMed 29341420
- Cell 168:1101-1113.e132017Complement Component 3 Adapts the Cerebrospinal Fluid for Leptomeningeal Metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdrienne Boire,Yilong Zou,Jason Shieh,Danilo G Macalinao,Elena Pentsova,Joan MassaguéPubMed 28283064
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 1.1 - 800 pg/mL
- Sensitivity
- < 2 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
Alternative names
Sdgf, Areg, Amphiregulin, AR, Schwannoma-derived growth factor, SDGF
What's included?
Recommended products
Mouse Amphiregulin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse Amphiregulin in Mouse in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 1.1 - 800 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 2 pg/mL
- General recovery
- 100%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 85.25 | Range 76 - 95 % |
Sample type Serum | Average % = 130.1 | Range 120 - 138 % |
Sample type Plasma | Average % = 123.4 | Range 112 - 130 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's Amphiregulin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Amphiregulin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse Amphiregulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Amphiregulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Amphiregulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Amphiregulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
'Amphiregulin' also known as 'AREG' or 'Amphiregulin protein' is a member of the epidermal growth factor (EGF) family. It is a 252 amino acid protein with a predicted mass of approximately 27-29 kDa. Amphiregulin shows expression in various tissues including epithelial cells fibroblasts and immune cells. This protein functions as a ligand for the EGF receptor (EGFR) leading to receptor dimerization and subsequent activation of downstream signaling pathways.
- Biological function summary
'Amphiregulin' plays a critical role in cell proliferation differentiation and tissue remodeling. It is not part of a constitutive complex but rather exerts its effects through interaction with EGFR a receptor tyrosine kinase. Amphiregulin contributes significantly to mammary gland development and has a notable role in wound healing. Beyond its developmental roles Amphiregulin can impact immune modulation and inflammation processes.
- Pathways
'Amphiregulin' significantly contributes to the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) pathways. These pathways influence cell survival growth and proliferation. Amphiregulin interacts with EGFR and by extension has relationships with other EGFR ligands such as EGF and transforming growth factor-alpha (TGF-alpha). These pathways underline the importance of Amphiregulin in maintaining cellular functions and responding to external stimuli.
- Associated diseases and disorders
'Amphiregulin' has strong associations with cancer and asthma. Overexpression of Amphiregulin can lead to increased cancer cell proliferation and it has been implicated in diverse cancers such as breast and colorectal cancer. Additionally the protein plays a role in airway remodeling in asthma contributing to bronchial hyper-responsiveness. In these contexts Amphiregulin's interactions with EGFR are central influencing disease progression and response to treatment.
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1 product image
Sandwich ELISA - Mouse Amphiregulin ELISA Kit (ab100668)
Representative Standard Curve using ab100668.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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