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AB100668

Mouse Amphiregulin ELISA Kit

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(3 Publications)

Mouse Amphiregulin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse Amphiregulin in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Sdgf, Areg, Amphiregulin, AR, Schwannoma-derived growth factor, SDGF

1 Images
Sandwich ELISA - Mouse Amphiregulin ELISA Kit (AB100668)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Amphiregulin ELISA Kit (AB100668)

Representative Standard Curve using ab100668.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 2 pg/mL

Range

1.1 - 800 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Amphiregulin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Amphiregulin in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse Amphiregulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Amphiregulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Amphiregulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Amphiregulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "76 - 95 %", "average": "= 85.25" }, { "sample": "Serum", "range": "120 - 138 %", "average": "= 130.1" }, { "sample": "Plasma", "range": "112 - 130 %", "average": "= 123.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100668-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100668-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100668-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100668-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100668-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse Amphiregulin", "number":"AB100668-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Amphiregulin Microplate (12 x 8 wells)", "number":"AB100668-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100668-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse Amphiregulin Standard (lyophilized)", "number":"AB100668-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'Amphiregulin' also known as 'AREG' or 'Amphiregulin protein' is a member of the epidermal growth factor (EGF) family. It is a 252 amino acid protein with a predicted mass of approximately 27-29 kDa. Amphiregulin shows expression in various tissues including epithelial cells fibroblasts and immune cells. This protein functions as a ligand for the EGF receptor (EGFR) leading to receptor dimerization and subsequent activation of downstream signaling pathways.
Biological function summary

'Amphiregulin' plays a critical role in cell proliferation differentiation and tissue remodeling. It is not part of a constitutive complex but rather exerts its effects through interaction with EGFR a receptor tyrosine kinase. Amphiregulin contributes significantly to mammary gland development and has a notable role in wound healing. Beyond its developmental roles Amphiregulin can impact immune modulation and inflammation processes.

Pathways

'Amphiregulin' significantly contributes to the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) pathways. These pathways influence cell survival growth and proliferation. Amphiregulin interacts with EGFR and by extension has relationships with other EGFR ligands such as EGF and transforming growth factor-alpha (TGF-alpha). These pathways underline the importance of Amphiregulin in maintaining cellular functions and responding to external stimuli.

'Amphiregulin' has strong associations with cancer and asthma. Overexpression of Amphiregulin can lead to increased cancer cell proliferation and it has been implicated in diverse cancers such as breast and colorectal cancer. Additionally the protein plays a role in airway remodeling in asthma contributing to bronchial hyper-responsiveness. In these contexts Amphiregulin's interactions with EGFR are central influencing disease progression and response to treatment.
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Product protocols

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Target data

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
See full target information Areg
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Immunity 51:899-914.e7 PubMed31732166

2019

Tissue-Specific Macrophage Responses to Remote Injury Impact the Outcome of Subsequent Local Immune Challenge.

Applications

Unspecified application

Species

Unspecified reactive species

Friedrich Felix Hoyer,Kamila Naxerova,Maximilian J Schloss,Maarten Hulsmans,Anil V Nair,Partha Dutta,David M Calcagno,Fanny Herisson,Atsushi Anzai,Yuan Sun,Gregory Wojtkiewicz,David Rohde,Vanessa Frodermann,Katrien Vandoorne,Gabriel Courties,Yoshiko Iwamoto,Christopher S Garris,David L Williams,Sylvie Breton,Dennis Brown,Michael Whalen,Peter Libby,Mikael J Pittet,Kevin R King,Ralph Weissleder,Filip K Swirski,Matthias Nahrendorf

Genes to cells : devoted to molecular & cellular mechanisms 23:136-145 PubMed29341420

2018

Transgenic mice specifically expressing amphiregulin in white adipose tissue showed less adipose tissue mass.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Yang,Takahiro Kumoto,Takeshi Arima,Minako Nakamura,Yohei Sanada,Thanutchaporn Kumrungsee,Yusuke Sotomaru,Masayuki Shimada,Noriyuki Yanaka

Cell 168:1101-1113.e13 PubMed28283064

2017

Complement Component 3 Adapts the Cerebrospinal Fluid for Leptomeningeal Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Adrienne Boire,Yilong Zou,Jason Shieh,Danilo G Macalinao,Elena Pentsova,Joan Massagué
View all publications
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