AB208349

Mouse Angiogenin ELISA Kit

Recombinant
SimpleStep
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(4 Publications)

Mouse Angiogenin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Angiogenin with a sensitivity of 16.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Ang1, Rnase5, Rnase5a, Ang, Angiogenin, Angiogenin-1, Ribonuclease 5, RNase 5

3 Images

Sandwich ELISA - Mouse Angiogenin ELISA Kit (AB208349)

sELISA

Supplier Data

Sandwich ELISA - Mouse Angiogenin ELISA Kit (AB208349)

Example of mouse Angiogenin standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Angiogenin ELISA Kit (AB208349)

Interpolated concentrations of native Angiogenin in mouse serum and mouse liver supernatant and spiked recombinant Angiogenin in cell culture media.

The concentrations of Angiogenin were measured in duplicates, interpolated from the Angiogenin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1.25%, mouse liver supernatant 20% and cell culture media 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 295.7 ng/mL in serum, 16.2 ng/mL in mouse liver supernatant and 10.3 ng/mL in cell culture media. Mouse liver was cultured for 5 days in RPMI base media with 10% fetal bovine serum and supernatants were collected according to section 11.3.

sELISA

Supplier Data

Sandwich ELISA - Mouse Angiogenin ELISA Kit (AB208349)

Interpolated concentrations of native Angiogenin in mouse plasma samples.

The concentrations of Angiogenin were measured in duplicates, interpolated from the Angiogenin standard curves and corrected for sample dilution. Undiluted samples are as follows : plasma (citrate) 1%, plasma (EDTA) 1.25%, and plasma (heparin) 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 342.4 ng/mL in plasma (citrate), 275.4 ng/mL in plasma (EDTA) and 375.6 ng/mL in plasma (heparin).

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 16.7 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Angiogenin ELISA Kit (ab208349) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Angiogenin protein in cell culture supernatant, serum, and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Angiogenin with 16.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Mouse Angiogenin is a member of the pancreatic ribonuclease A superfamily and is a potent inducer of neovascularization. Furthermore, Angiogenin is essential for cell growth and proliferation. Angiogenin direct binding to the promoter region of ribosomal DNA induces ribosomal RNA transcription and cell proliferation required for ribosomal biogenesis and the action of angiogenic factors. Increased Angiogenin serum levels have been associated with the incidence and severity of several types of tumors. Mouse Angiogenin is 145 amino acids (aa) in length, with a 24 aa signal peptide, and contains three intra-chain disulfide bonds. Human and rat Angiogenin are 74% and 83% identical to mouse Angiogenin, respectively.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.3" } ]

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Recovery

[ { "sample": "Serum", "range": "105 - 107 %", "average": "= 106" }, { "sample": "Cell culture media", "range": "100 - 103 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "92 - 110 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "105 - 110 %", "average": "= 107" }, { "sample": "Heparin Plasma", "range": "104 - 108 %", "average": "= 106" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiogenin also known as ANG is a small protein with a molecular mass of approximately 14 kDa. It belongs to the ribonuclease A superfamily. Angiogenin expression occurs in a variety of tissues with notably pronounced levels in the liver pancreas and placenta indicating its widespread biological importance. In addition to its primary name it appears in the context of research under alternative names like KIT ANG and elisa ang reflecting its use and identification in various experimental assays.

Biological function summary

Angiogenin significantly contributes to the process of angiogenesis the formation of new blood vessels from pre-existing vascular structures. It characterizes acts as both a ribonuclease and a potent angiogenic factor. Although not part of a multi-protein complex angiogenin interacts with endothelial cells to promote their growth and migration. Angiogenin facilitates these cellular behaviors by triggering pathways that enhance cell survival and proliferation essential for healthy tissue development and repair.

Pathways

Angiogenin is intricately involved in the angiogenesis pathway a critical process for both physiological and pathological conditions. It interacts with proteins such as VEGF (vascular endothelial growth factor) which plays an essential role in vascular function. Angiogenin's interaction with these pathways enhances cellular responses to external stimuli maintaining vascular integrity and supporting tissue regeneration under stress or injury conditions.

Angiogenin has connections to conditions like cancer and amyotrophic lateral sclerosis (ALS). Aberrant regulation of angiogenin can contribute to tumorigenesis by promoting excessive angiogenesis aiding the growth and spread of cancerous cells. In ALS altered angiogenin function associates with neurodegenerative processes linking its function to proteins such as superoxide dismutase 1 (SOD1). Understanding angiogenin's role in these diseases aids in uncovering therapeutic targets and developing treatment strategies.

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Product protocols

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Target data

Secreted ribonuclease that can either promote or restrict cell proliferation of target cells, depending on the context (PubMed : 19114040, PubMed : 27518564, PubMed : 32510170, PubMed : 33758201, PubMed : 33986151). Endocytosed in target cells via its receptor PLXNB2 and translocates to the cytoplasm or nucleus (PubMed : 29100074, PubMed : 33758201). Under stress conditions, localizes to the cytoplasm and promotes the assembly of stress granules (SGs) : specifically cleaves a subset of tRNAs within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs), resulting in translation repression and inhibition of cell proliferation (PubMed : 19114040, PubMed : 24752898, PubMed : 33986151). tiRNas also prevent formation of apoptosome, thereby promoting cell survival (PubMed : 24752898). Preferentially cleaves RNAs between a pyrimidine and an adenosine residue, suggesting that it cleaves the anticodon loop of tRNA(Ala) (32-UUAGCAU-38) after positions 33 and 36 (By similarity). Cleaves a subset of tRNAs, including tRNA(Ala), tRNA(Glu), tRNA(Gly), tRNA(Lys), tRNA(Val), tRNA(His), tRNA(Asp) and tRNA(Sec) (By similarity). Under growth conditions and in differentiated cells, translocates to the nucleus and stimulates ribosomal RNA (rRNA) transcription, including that containing the initiation site sequences of 45S rRNA, thereby promoting cell growth and proliferation (By similarity). Angiogenin induces vascularization of normal and malignant tissues via its ability to promote rRNA transcription (By similarity). Involved in hematopoietic stem and progenitor cell (HSPC) growth and survival by promoting rRNA transcription in growth conditions and inhibiting translation in response to stress, respectively (PubMed : 27518564). Mediates the crosstalk between myeloid and intestinal epithelial cells to protect the intestinal epithelial barrier integrity : secreted by myeloid cells and promotes intestinal epithelial cells proliferation and survival (PubMed : 32510170). Also mediates osteoclast-endothelial cell crosstalk in growing bone : produced by osteoclasts and protects the neighboring vascular cells against senescence by promoting rRNA transcription (PubMed : 33758201).

See full target information Ang

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 10:207-221 PubMed34901540

2021

A bone-targeted engineered exosome platform delivering siRNA to treat osteoporosis.

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Species

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Yongzhi Cui,Yuanyuan Guo,Li Kong,Jingyu Shi,Ping Liu,Rui Li,Yongtao Geng,Weihang Gao,Zhiping Zhang,Dehao Fu

Molecular cancer research : MCR 20:244-252 PubMed34728552

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A Functional Precision Oncology Approach to Identify Treatment Strategies for Myxofibrosarcoma Patients.

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Species

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Chantal Pauli,Lamberto De Boni,Jonathan E Pauwels,Yanjiang Chen,Lara Planas-Paz,Reid Shaw,Brooke M Emerling,Carla Grandori,Benjamin D Hopkins,Mark A Rubin

Frontiers in neurology 12:662235 PubMed34234733

2021

Angiogenin in the Neurogenic Subventricular Zone After Stroke.

Applications

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Species

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Marina Gabriel-Salazar,Ting Lei,Alba Grayston,Carme Costa,Esperanza Medina-Gutiérrez,Manuel Comabella,Joan Montaner,Anna Rosell

Nature communications 12:1832 PubMed33758201

2021

Osteoclasts protect bone blood vessels against senescence through the angiogenin/plexin-B2 axis.

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Species

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Xiaonan Liu,Yu Chai,Guanqiao Liu,Weiping Su,Qiaoyue Guo,Xiao Lv,Peisong Gao,Bin Yu,Gerardo Ferbeyre,Xu Cao,Mei Wan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse Angiogenin ELISA Kit

Sandwich ELISA - Mouse Angiogenin ELISA Kit (AB208349)

Sandwich ELISA - Mouse Angiogenin ELISA Kit (AB208349)

Sandwich ELISA - Mouse Angiogenin ELISA Kit (AB208349)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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