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AB171335

Mouse Angiopoietin 2 ELISA Kit

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(5 Publications)

Mouse Angiopoietin 2 ELISA Kit is a Sandwich ELISA for the measurement of Mouse Angiopoietin 2 in Mouse in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

Agpt2, Angpt2, Angiopoietin-2, ANG-2

3 Images
Sandwich ELISA - Mouse Angiopoietin 2 ELISA Kit (AB171335)
  • sELISA

Lab

Sandwich ELISA - Mouse Angiopoietin 2 ELISA Kit (AB171335)
Sandwich ELISA - Mouse Angiopoietin 2 ELISA Kit (AB171335)
  • sELISA

Lab

Sandwich ELISA - Mouse Angiopoietin 2 ELISA Kit (AB171335)
Sandwich ELISA - Mouse Angiopoietin 2 ELISA Kit (AB171335)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Angiopoietin 2 ELISA Kit (AB171335)

Representative standard curve using ab171335

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

156 - 10000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's mouse Angiopoietin-2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse Angiopoietin-2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

An Angiopoietin-2 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Angiopoietin-2 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Angiopoietin-2 amount of sample captured in plate.

Get better reproducibility in only 90 minutes with Mouse Angiopoietin 2 ELISA Kit (ab209883) from our SimpleStep ELISA® range.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "1185 pg/mL", "standardDeviation": "54.51", "coefficientOfVariability": "= 4.6" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "210 pg/mL", "standardDeviation": "10.5", "coefficientOfVariability": "= 5" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "4889 pg/mL", "standardDeviation": "303.11", "coefficientOfVariability": "= 6.2" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "4765 pg/mL", "standardDeviation": "274.67", "coefficientOfVariability": "= 5.8" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "230 pg/mL", "standardDeviation": "11.5", "coefficientOfVariability": "= 5" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "1256 pg/mL", "standardDeviation": "55.06", "coefficientOfVariability": "= 4.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab171335-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB171335-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-mouse Angiopoietin 2 antibody", "number":"AB171335-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-mouse Angiopoietin-2 Antibody Microplate (12 x 8 wells)", "number":"AB171335-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB171335-CMP06", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB171335-CMP10", "productcode":"" }, { "size":"1 x 4 Unit", "name":"Plate Seal", "number":"AB171335-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB171335-CMP08", "productcode":"" }, { "size":"2 x 10 ng", "name":"Lyophilized recombinant mouse Angiopoietin 2 standard", "number":"AB171335-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB171335-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB171335-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB171335-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiopoietin-2 also known as ANG2 or ANGPT2 is a glycoprotein with a molecular mass of approximately 70 kDa. It plays an important role in angiogenesis facilitating blood vessel remodeling and maturation. The protein is mainly expressed in endothelial cells where it functions by binding to the Tie2 receptor partially disrupting the Angiopoietin-Tie2 signaling axis. This disruption modulates vascular stability and permeability. The lyophilized form of the ANG-2 protein is often used in research for functional assays like ANG-2 ELISA to quantify its levels in various biological samples.
Biological function summary

Human angiogenesis processes are significantly affected by Angiopoietin-2 which serves as an antagonist to Angiopoietin-1 in regulating vascular dynamics. Although it does not form a stable complex by itself ANG2 activity is closely linked to its interactions with the Tie2 receptor modulating signal pathways that govern endothelial cell survival and permeability. Because of its dual role either stabilizing or destabilizing blood vessels under different conditions ANG2 serves as an important regulator during both normal physiological events and pathophysiological conditions.

Pathways

Angiopoietin-2 significantly contributes to the angiogenesis signaling network. It is deeply involved in the VEGF (Vascular Endothelial Growth Factor) pathway where ANG2 acts to balance VEGF activities in angiogenesis and vessel permeability. The interplay between Angiopoietin-2 and other proteins such as Angiopoietin-1 which stabilizes the vessels is vital for the precise regulation of blood vessel homeostasis during normal and disturbed conditions.

Angiopoietin-2 has a prominent role in the pathology of diseases like cancer and diabetic retinopathy. In cancer excessive ANG2 expression may lead to increased tumor angiogenesis and metastasis making it a target for cancer therapies. In diabetic retinopathy elevated levels of ANG2 correlate with vascular leakage contributing to disease progression. The relationship between Angiopoietin-1 and Angiopoietin-2 in these conditions emphasizes their collective impact on vascular anomalies associated with these disorders.
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Product protocols

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Target data

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling (By similarity). Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1 (By similarity). In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression (By similarity). In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal (By similarity). Involved in the regulation of lymphangiogenesis (PubMed : 28179430, PubMed : 32908006).
See full target information Angpt2
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 24:e55532 PubMed36621885

2023

T cell-dependent bispecific antibodies alter organ-specific endothelial cell-T cell interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia Himmels,Thi Thu Thao Nguyen,Maresa Caunt Mitzner,Alfonso Arrazate,Stacey Yeung,Jeremy Burton,Robyn Clark,Klara Totpal,Raj Jesudason,Angela Yang,Margaret Solon,Jeffrey Eastham,Zora Modrusan,Joshua D Webster,Amy A Lo,Robert Piskol,Weilan Ye

Cancers 14: PubMed35626052

2022

Acquired αSMA Expression in Pericytes Coincides with Aberrant Vascular Structure and Function in Pancreatic Ductal Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Vikneshwari Natarajan,Sangdeuk Ha,Alexander Delgado,Reed Jacobson,Lina Alhalhooly,Yongki Choi,Jiha Kim

Shock (Augusta, Ga.) 57:608-615 PubMed34907117

2021

Patho-Mechanisms for Hemorrhage/Sepsis-Induced Indirect Acute Respiratory Distress Syndrome: A Role for Lung TIE1 and Its Regulation by Neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Jiali Zhu,Jinbao Li,Chun-Shiang Chung,Joanne L Lomas-Neira,Alfred Ayala

JCI insight 1:e90733 PubMed28018977

2016

Heterogeneous perivascular cell coverage affects breast cancer metastasis and response to chemotherapy.

Applications

ELISA

Species

Human

Jiha Kim,Pedro Correa de Sampaio,Donna Marie Lundy,Qian Peng,Kurt W Evans,Hikaru Sugimoto,Mihai Gagea,Yvonne Kienast,Nayra Soares do Amaral,Rafael Malagoli Rocha,Hans Petter Eikesdal,Per Eystein Lønning,Funda Meric-Bernstam,Valerie S LeBleu

Nature medicine 22:1033-1042 PubMed27548575

2016

Endothelial exocytosis of angiopoietin-2 resulting from CCM3 deficiency contributes to cerebral cavernous malformation.

Applications

Unspecified application

Species

Unspecified reactive species

Huanjiao Jenny Zhou,Lingfeng Qin,Haifeng Zhang,Wenwen Tang,Weidong Ji,Yun He,Xiaoling Liang,Zongren Wang,Qianying Yuan,Alexander Vortmeyer,Derek Toomre,Germaine Fuh,Minghong Yan,Martin S Kluger,Dianqing Wu,Wang Min
View all publications
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