AB209883

Mouse Angiopoietin 2 ELISA Kit

Recombinant
SimpleStep
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(3 Publications)

Mouse Angiopoietin 2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Angiopoietin 2 with a sensitivity of 17.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Agpt2, Angpt2, Angiopoietin-2, ANG-2

2 Images

Sandwich ELISA - Mouse Angiopoietin 2 ELISA Kit (AB209883)

sELISA

Supplier Data

Sandwich ELISA - Mouse Angiopoietin 2 ELISA Kit (AB209883)

Example of mouse Angiopoietin 2 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Mouse Angiopoietin 2 ELISA Kit (AB209883)

Interpolated concentrations of native Angiopoietin 2 in mouse and rat serum and mouse plasma samples.

The concentrations of Angiopoietin 2 were measured in duplicates, interpolated from the Angiopoietin 2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 6%, plasma (EDTA) 7.5%, plasma (heparin) 7.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiopoietin 2 concentration was determined to be 19,017 pg/mL in mouse serum, 2,802 pg/mL in rat serum, 31,192 pg/mL in mouse plasma (citrate), 26,451 pg/mL in mouse plasma (EDTA) and 33,857 pg/mL in mouse plasma (heparin).

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 17.3 pg/mL

Range

68.75 - 4400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Angiopoietin 2 ELISA Kit (ab209883) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Angiopoietin 2 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Angiopoietin 2 with 17.3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Angiopoietin 2 is a secreted glycosylated protein functioning in angiogenesis, inflammation and vascular development. Angiopoietin 2 binds to receptor tyrosine kinase TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signalling. Angiopoietin 2 can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, Angiopoietin 2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, Angiopoietin 2 may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Mouse Plasma", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.3" }, { "reproducibilityType": "Intra", "sample": "Mouse Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.3" } ]

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Recovery

[ { "sample": "Serum", "range": "67.4 - 120.9 %", "average": "= 94.5" }, { "sample": "EDTA Plasma", "range": "91.8 - 122.8 %", "average": "= 104.9" }, { "sample": "Heparin Plasma", "range": "81.4 - 143.2 %", "average": "= 112.4" }, { "sample": "Citrate plasma", "range": "87.1 - 103.3 %", "average": "= 94.7" }, { "sample": "Cell culture media", "range": "94.3 - 103.3 %", "average": "= 99.7" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiopoietin-2 also known as ANG2 or ANGPT2 is a glycoprotein with a molecular mass of approximately 70 kDa. It plays an important role in angiogenesis facilitating blood vessel remodeling and maturation. The protein is mainly expressed in endothelial cells where it functions by binding to the Tie2 receptor partially disrupting the Angiopoietin-Tie2 signaling axis. This disruption modulates vascular stability and permeability. The lyophilized form of the ANG-2 protein is often used in research for functional assays like ANG-2 ELISA to quantify its levels in various biological samples.

Biological function summary

Human angiogenesis processes are significantly affected by Angiopoietin-2 which serves as an antagonist to Angiopoietin-1 in regulating vascular dynamics. Although it does not form a stable complex by itself ANG2 activity is closely linked to its interactions with the Tie2 receptor modulating signal pathways that govern endothelial cell survival and permeability. Because of its dual role either stabilizing or destabilizing blood vessels under different conditions ANG2 serves as an important regulator during both normal physiological events and pathophysiological conditions.

Pathways

Angiopoietin-2 significantly contributes to the angiogenesis signaling network. It is deeply involved in the VEGF (Vascular Endothelial Growth Factor) pathway where ANG2 acts to balance VEGF activities in angiogenesis and vessel permeability. The interplay between Angiopoietin-2 and other proteins such as Angiopoietin-1 which stabilizes the vessels is vital for the precise regulation of blood vessel homeostasis during normal and disturbed conditions.

Angiopoietin-2 has a prominent role in the pathology of diseases like cancer and diabetic retinopathy. In cancer excessive ANG2 expression may lead to increased tumor angiogenesis and metastasis making it a target for cancer therapies. In diabetic retinopathy elevated levels of ANG2 correlate with vascular leakage contributing to disease progression. The relationship between Angiopoietin-1 and Angiopoietin-2 in these conditions emphasizes their collective impact on vascular anomalies associated with these disorders.

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Product protocols

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Target data

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling (By similarity). Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1 (By similarity). In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression (By similarity). In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal (By similarity). Involved in the regulation of lymphangiogenesis (PubMed : 28179430, PubMed : 32908006).

See full target information Angpt2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 10: PubMed35740360

2022

Angiopoietin-2 Inhibition of Thrombomodulin-Mediated Anticoagulation-A Novel Mechanism That May Contribute to Hypercoagulation in Critically Ill COVID-19 Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Hultström,Karin Fromell,Anders Larsson,Barbro Persson,Bo Nilsson,Susan E Quaggin,Christer Betsholtz,Robert Frithiof,Miklos Lipcsey,Marie Jeansson

Molecular biology reports 46:5841-5858 PubMed31396803

2019

Testing alternatives: the use of adipose-derived mesenchymal stem cells to slow neurodegeneration in a rat model of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Fatma Y Meligy,Dalia A Elgamal,Eman S H Abd Allah,Naglaa K Idriss,Nagwa M Ghandour,Ehab M R Bayoumy,Azza Sayed Abdelrehim Khalil,Mohamed M El Fiky,Mostafa Elkhashab

Stem cells international 2017:3794817 PubMed29238372

2017

Transplantation of Rat Mesenchymal Stem Cells Overexpressing Hypoxia-Inducible Factor 2 Improves Blood Perfusion and Arteriogenesis in a Rat Hindlimb Ischemia Model.

Applications

Unspecified application

Species

Unspecified reactive species

Weifeng Lu,Xiaoli Chen,Yi Si,Shichai Hong,Zhengyu Shi,Weiguo Fu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse Angiopoietin 2 ELISA Kit

Sandwich ELISA - Mouse Angiopoietin 2 ELISA Kit (AB209883)

Sandwich ELISA - Mouse Angiopoietin 2 ELISA Kit (AB209883)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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