AB210577

Mouse Angiopoietin-like 4 ELISA Kit

Recombinant
SimpleStep
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Mouse Angiopoietin-like 4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Angiopoietin-like 4 with a sensitivity of 29 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Farp, Fiaf, Ng27, Angptl4, Angiopoietin-related protein 4, 425O18-1, Angiopoietin-like protein 4, Fasting-induced adipose factor, Hepatic fibrinogen/angiopoietin-related protein, Secreted protein Bk89, HFARP

6 Images

Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit (AB210577)

sELISA

Supplier Data

Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit (AB210577)

Interpolated concentrations of native Angiopoietin-related protein 4 in mouse cell and tissue culture supernatant samples.

The concentrations of Angiopoietin-related protein 4 were measured in duplicates, interpolated from the Angiopoietin-related protein 4 standard curves and corrected for sample dilution. Undiluted samples are as follows : lung supernatant 20%, heart supernatant 50%, and NIH3T3 Supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiopoietin-related protein 4 concentration was determined to be 9,986 pg/mL in lung supernatant, 606 pg/mL in heart supernatant and 1,030 pg/mL in NIH3T3 cell culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit (AB210577)

Example of Mouse Angiopoietin-related protein 4 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit (AB210577)

Comparison of secreted Angiopoietin-related protein 4 in unstimulated and PDGF- stimulated NIH/3T3 supernatant.

NIH/3T3 cells were grown in the absence or presence of Platelet Derived Growth Factor (PDGF) for 24 hours. The concentrations of Angiopoietin-related protein 4 were measured in duplicates, interpolated from the Angiopoietin-related protein 4 standard curves and corrected for sample dilution. Undiluted samples are 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiopoietin-related protein 4 concentration was determined to be 4,704 pg/mL in unstimulated, 20,700 pg/ml in PDGF stimulated and undetectable in media.

sELISA

Supplier Data

Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit (AB210577)

Interpolated concentrations of native Angiopoietin-related protein 4 in mouse liver extract sample based on a 2,000 μg/mL extract load.

The concentrations of Angiopoietin-related protein 4 were measured in duplicate and interpolated from the Angiopoietin-related protein 4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiopoietin-related protein 4 concentration was determined to be 748 pg/mL in mouse liver extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit (AB210577)

Interpolated concentrations of native Angiopoietin-related protein 4 in mouse tissue culture supernatant samples.

The concentrations of Angiopoietin-related protein 4 were measured in duplicates, interpolated from the Angiopoietin-related protein 4 standard curves and corrected for sample dilution. Undiluted samples are 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiopoietin-related protein 4 concentration was determined to be 10,668 pg/mL in lung supernatant, 2,884pg/mL in liver supernatant, 2,703pg/mL in brain supernatant and 456 pg/mL in heart supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit (AB210577)

Example of Mouse Angiopoietin-related protein 4 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Cell culture supernatant

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 29 pg/mL

Range

117.2 - 7500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Angiopoietin-like 4 ELISA Kit (ab210577) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Angiopoietin-like 4 protein in cell culture extracts, cell culture supernatant, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Angiopoietin-like 4 with 29 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Angiopoietin-like 4 (Angiopoietin-related protein 4,ANGPTL4) is a member of the ANGPTL family and structurally related to angiopoietins. Angiopoietin-like 4 is secreted and may also associate with the extracellular matrix. It is associated with angiogenesis and metastasis.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.6" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]

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Recovery

[ { "sample": "Tissue Extracts", "range": "105 - 114 %", "average": "= 108" }, { "sample": "Cell culture media", "range": "98 - 109 %", "average": "= 103" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiopoietin-like 4 also known as ANGPTL4 like4 or ANGPTL4 protein is a glycoprotein with an approximate mass of 50 kDa. It plays a role in lipid metabolism by inhibiting lipoprotein lipase which reduces the breakdown of triglycerides. ANGPTL4 protein is secreted by liver adipose tissue and placenta showing expression in response to fasting and during low insulin states. This expression pattern connects its function to energy homeostasis and metabolic regulation.

Biological function summary

ANGPTL4 protein influences processes such as lipid metabolism angiogenesis and wound healing. It does not integrate into a large protein complex but functions independently to exert its effects. Inhibition of lipoprotein lipase by ANGPTL4 decreases adipose tissue fat storage and modulates plasma lipid levels. This modulation plays a critical role in maintaining energy balance and glucose homeostasis particularly under metabolic stress conditions.

Pathways

The functionality of ANGPTL4 protein intersects with the PPAR (Peroxisome Proliferator-Activated Receptor) signaling pathway and the glucose homeostasis pathway. It interacts with other proteins such as PPAR-alpha and PPAR-gamma which regulate genes involved in fatty acid oxidation and lipid transport. This interaction influences the body's response to fasting and caloric restriction by altering lipid metabolism and ensuring efficient energy use.

ANGPTL4 protein correlates with cardiovascular diseases and cancer. Elevated levels of ANGPTL4 associate with an increased risk for atherosclerosis due to its role in lipid metabolism disturbance. In cancer particularly gastric cancer ANGPTL4 influences tumor metastasis by promoting angiogenesis. Its effects on these diseases link to proteins like VEGF in angiogenesis and other metabolic regulators in cardiovascular conditions.

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Product protocols

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Target data

Mediates inactivation of the lipoprotein lipase LPL, and thereby plays a role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism (PubMed : 15837923, PubMed : 17609370, PubMed : 29899519). May also play a role in regulating glucose homeostasis and insulin sensitivity (PubMed : 15837923, PubMed : 29899519). Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage (PubMed : 14583458, PubMed : 17130448, PubMed : 21832056). Upon heterologous expression, inhibits the adhesion of endothelial cell to the extracellular matrix (ECM), and inhibits the reorganization of the actin cytoskeleton, formation of actin stress fibers and focal adhesions in endothelial cells that have adhered to ANGPTL4-containing ECM (in vitro) (By similarity). Depending on context, may modulate tumor-related angiogenesis (Probable).. ANGPTL4 N-terminal chain. Mediates inactivation of the lipoprotein lipase LPL, and thereby plays an important role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism. Has higher activity in LPL inactivation than the uncleaved protein.

See full target information Angptl4

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websiteProtocolBooklet

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Mouse Angiopoietin-like 4 ELISA Kit

Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit (AB210577)

Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit (AB210577)

Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit (AB210577)

Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit (AB210577)

Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit (AB210577)

Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit (AB210577)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Product promise