JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB326887

Mouse Angiopoietin-like 4 ELISA Kit, Chemiluminescent

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse Angiopoietin-like 4 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse Angiopoietin-like 4 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 41.15 - 30000 pg/ml with a sensitivity of 16.46 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

Farp, Fiaf, Ng27, Angptl4, Angiopoietin-related protein 4, 425O18-1, Angiopoietin-like protein 4, Fasting-induced adipose factor, Hepatic fibrinogen/angiopoietin-related protein, Secreted protein Bk89, HFARP

5 Images
Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit, Chemiluminescent (AB326887)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit, Chemiluminescent (AB326887)

Interpolated concentrations of native Angiopoietin-related protein 4 in mouse tissue culture supernatant samples.

The concentrations of Angiopoietin-related protein 4 were measured in duplicates, interpolated from the Angiopoietin-related protein 4 standard curves and corrected for sample dilution. Undiluted samples are 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiopoietin-related protein 4 concentration was determined to be 10,668 pg/mL in lung supernatant, 2,884pg/mL in liver supernatant, 2,703pg/mL in brain supernatant and 456 pg/mL in heart supernatant.

Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit, Chemiluminescent (AB326887)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit, Chemiluminescent (AB326887)

Comparison of secreted Angiopoietin-related protein 4 in unstimulated and PDGF- stimulated NIH/3T3 supernatant.

NIH/3T3 cells were grown in the absence or presence of Platelet Derived Growth Factor (PDGF) for 24 hours. The concentrations of Angiopoietin-related protein 4 were measured in duplicates, interpolated from the Angiopoietin-related protein 4 standard curves and corrected for sample dilution. Undiluted samples are 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiopoietin-related protein 4 concentration was determined to be 4,704 pg/mL in unstimulated, 20,700 pg/ml in PDGF stimulated and undetectable in media.

Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit, Chemiluminescent (AB326887)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit, Chemiluminescent (AB326887)

Interpolated concentrations of native Angiopoietin-related protein 4 in mouse cell and tissue culture supernatant samples.

The concentrations of Angiopoietin-related protein 4 were measured in duplicates, interpolated from the Angiopoietin-related protein 4 standard curves and corrected for sample dilution. Undiluted samples are as follows : lung supernatant 20%, heart supernatant 50%, and NIH3T3 Supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiopoietin-related protein 4 concentration was determined to be 9,986 pg/mL in lung supernatant, 606 pg/mL in heart supernatant and 1,030 pg/mL in NIH3T3 cell culture supernatant.

Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit, Chemiluminescent (AB326887)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit, Chemiluminescent (AB326887)

Interpolated concentrations of native Angiopoietin-related protein 4 in mouse liver extract sample based on a 2,000 μg/mL extract load.

The concentrations of Angiopoietin-related protein 4 were measured in duplicate and interpolated from the Angiopoietin-related protein 4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiopoietin-related protein 4 concentration was determined to be 748 pg/mL in mouse liver extract.

Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit, Chemiluminescent (AB326887)
  • sELISA

Lab

Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit, Chemiluminescent (AB326887)

Standard curve comparison between the Chemiluminescent Mouse Angiopoietin-like 4 SimpleStep ELISA and colorimetric Mouse Angiopoietin-like 4 SimpleStep ELISA (ab210577) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Luminescent

Sample types

Tissue Extracts, Cell culture media

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 16.46 pg/mL

Range

41.15 - 30000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Mouse Angiopoietin-like 4 ELISA Kit, Chemiluminescent (ab326887) is a rapid single-wash sandwich 55-min ELISA to measure Mouse Angiopoietin-like 4 in Tissue Extracts, Cell culture media with a sensitivity of 16.46 pg/mL.

How the assay works

Mouse Angiopoietin-like 4 ELISA Kit, Chemiluminescent (ab326887) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Angiopoietin-like 4 (ab326887) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-ANGPTL4 antibody [EPR18220-120] - BSA and Aside free (Capture) ab242552
- Anti-ANGPTL4 antibody [EPR18220-38] - BSA and Aside free (Detector) ab242800
style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.7" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.6" } ]
style
left-column

Recovery

[ { "sample": "Tissue Extracts", "range": "105 - 114 %", "average": "= 114" }, { "sample": "Cell culture media", "range": "98 - 109 %", "average": "= 109" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326887-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Mouse Angiopoietin-like 4 Detector Antibody", "number":"AB326887-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB326887-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326887-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB326887-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326887-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB326887-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Angiopoietin-like 4 Lyophilized Recombinant Protein", "number":"AB326887-CMP03", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326887-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB326887-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB326887-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Angiopoietin-like 4 Capture Antibody", "number":"AB326887-CMP01", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326887-CMP10", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Mediates inactivation of the lipoprotein lipase LPL, and thereby plays a role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism (PubMed : 15837923, PubMed : 17609370, PubMed : 29899519). May also play a role in regulating glucose homeostasis and insulin sensitivity (PubMed : 15837923, PubMed : 29899519). Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage (PubMed : 14583458, PubMed : 17130448, PubMed : 21832056). Upon heterologous expression, inhibits the adhesion of endothelial cell to the extracellular matrix (ECM), and inhibits the reorganization of the actin cytoskeleton, formation of actin stress fibers and focal adhesions in endothelial cells that have adhered to ANGPTL4-containing ECM (in vitro) (By similarity). Depending on context, may modulate tumor-related angiogenesis (Probable).. ANGPTL4 N-terminal chain. Mediates inactivation of the lipoprotein lipase LPL, and thereby plays an important role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism. Has higher activity in LPL inactivation than the uncleaved protein.
See full target information Angptl4
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com