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AB210576

Mouse Annexin A2 ELISA Kit

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Mouse Annexin A2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Annexin A2 with a sensitivity of 17 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Anx2, Cal1h, Anxa2, Annexin A2, Annexin II, Annexin-2, Calpactin I heavy chain, Calpactin-1 heavy chain, Chromobindin-8, Lipocortin II, Placental anticoagulant protein IV, Protein I, p36, PAP-IV

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Sandwich ELISA - Mouse Annexin A2 ELISA Kit (AB210576)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Annexin A2 ELISA Kit (AB210576)

Interpolated concentrations of native Annexin A2 in mouse lung day 5 cell culture supernatant sample.

The concentrations of Annexin A2 were measured in duplicate and interpolated from the Annexin A2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Annexin A2 concentration was determined to be 6,362 pg/mL in neat mouse lung supernatant sample.

Sandwich ELISA - Mouse Annexin A2 ELISA Kit (AB210576)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Annexin A2 ELISA Kit (AB210576)

Interpolated concentrations of native Annexin A2 in mouse NIH/3T3 cell extract samples based on 1 µg/mL extract load and in mouse heart extract samples based on 25 µg/mL extract load.

The concentrations of Annexin A2 were measured in duplicate and interpolated from the Annexin A2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Annexin A2 concentration was determined to be 5,627 pg/mL in NIH/3T3 cell extract and 8,735 pg/mL in mouse heart extract samples.

Sandwich ELISA - Mouse Annexin A2 ELISA Kit (AB210576)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Annexin A2 ELISA Kit (AB210576)

Example of mouse Annexin A2 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Annexin A2 ELISA Kit (AB210576)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Annexin A2 ELISA Kit (AB210576)

Example of mouse Annexin A2 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Annexin A2 ELISA Kit (AB210576)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Annexin A2 ELISA Kit (AB210576)

Interpolated concentrations of spiked Annexin A2 in mouse serum and plasma samples.

The concentrations of Annexin A2 were measured in duplicates, interpolated from the Annexin A2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 50%.The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 17 pg/mL

Range

78.13 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Proteins & Peptides

AB93005

Recombinant Human Annexin-2/ANXA2 protein

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  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Annexin A2 ELISA Kit (ab210576) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Annexin A2 protein in cell culture supernatant, plasma, serum, tissue extracts, cell culture media, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Annexin A2 with 17 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Annexin A2 is a secreted 36 kDa calcium-dependent phospholipid-binding protein localized on the surface of most eukaryotic cells. Annexin A2 is involved in several biological processes, including anti-inflammatory effects, Ca2+-dependent exocytosis, immune responses, Ca2+ transport and phospholipase A2 regulation. Annexin A2 forms a heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of Annexin A2/p36. Annexin A2 binds two calcium ions with high affinity. Annexin A2 inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Heart Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" }, { "reproducibilityType": "Intra", "sample": "Heart Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" } ]
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Recovery

[ { "sample": "Serum", "range": "90 - 101 %", "average": "= 97" }, { "sample": "Cell culture extracts", "range": "109 - 114 %", "average": "= 112" }, { "sample": "Cell culture media", "range": "106 - 120 %", "average": "= 113" }, { "sample": "Tissue Extracts", "range": "89 - 110 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "84 - 89 %", "average": "= 87" }, { "sample": "Heparin Plasma", "range": "83 - 86 %", "average": "= 84" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin-2 also known as ANXA2 is a multifunctional protein of approximately 36 kDa. It belongs to the annexin family and is known for its strong calcium-dependent interactions with phospholipid membranes. Commonly expressed in endothelial cells it also appears in various tissues including epithelial cells and certain types of cancer cells. ANXA2 forms a heterotetramer complex with S100A10 also known as p11 which modulates its availability and function. Other names for ANXA2 include its gene designation M298 and its role in complexes often goes by ANXA2R.
Biological function summary

Annexin-2 acts beyond mere cellular structural support. It plays a part in fibrinolysis by acting as a co-receptor for plasminogen and tissue plasminogen activator promoting plasmin generation. The protein is also implicated in membrane trafficking signal transduction and organization of microdomains on cellular surfaces. As part of a complex with S100A10 ANXA2 can bind to cytoskeletal elements facilitating vesicle trafficking and influencing cell shape and motility. This complex assembly hints at ANXA2's importance in maintaining cellular dynamism and interplay with external signals.

Pathways

ANXA2 is integral in the regulation of the plasminogen activation pathway important for fibrinolytic activity. It also participates in the signal transduction pathways influencing cellular responses to stress and growth factors. ANXA2 interacts with various proteins like Src kinase and is involved in processes like the EGFR signaling pathway. This protein often crosstalks with the cytoskeletal network enabling the coupling of membrane dynamics with intracellular signaling cascades.

Annexin-2's dysregulation associates with conditions such as cancer and thrombosis. In cancer overexpression of ANXA2 links with tumor progression invasion and metastasis acting synergistically with proteins like Src to promote oncogenic pathways. In thrombosis ANXA2’s role in fibrinolysis becomes impaired contributing to excessive clot formation. Studies also indicate interactions with plasminogen-related proteins as being central to understanding its pathological roles in these diseases.
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Product protocols

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Target data

Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response (By similarity). Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9 (PubMed : 22848640). Binds to endosomes damaged by phagocytosis of particulate wear debris and participates in endosomal membrane stabilization, thereby limiting NLRP3 inflammasome activation (PubMed : 22453828). Required for endothelial cell surface plasmin generation and may support fibrinolytic surveillance and neoangiogenesis (PubMed : 14702107).. (Microbial infection) Regulates the formation of the NLRC4 inflammasome triggered by Anaplasma phagocytophilum infection.. (Microbial infection) Protects against Klebsiella pneumoniae infection (PubMed : 26527544). Attenuates bacteria-induced pulmonary inflammation and promotes intro-abdominal pathogen clearance (PubMed : 26527544). Promotes anti-inflammatory responses by facilitating TLR4 internalization and translocation into early endosomal membranes; this leads to activation of TRAM-dependent endosomal signaling and release of anti-inflammatory cytokines (PubMed : 26527544).. (Microbial infection) Promotes macrophage phagocytic efficiency towards Cryptococcus neoformans and ability to control fungal infection inside the cells.. (Microbial infection) Contributes to protection against Pseudomonas aeruginosa infection by regulating autophagy via the AKT1-mTOR-ULK1/2 signaling pathway and activation of Rho GTPases via FAM13A-mediated mechanism.
See full target information Anxa2
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Product promise

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