AB230932

Mouse Apo B ELISA Kit

Recombinant
SimpleStep
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(17 Publications)

Mouse Apo B ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Apo B with a sensitivity of 196 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

View Alternative Names

Apolipoprotein B-100, Apo B-100, Apob, Apolipoprotein B100

7 Images

ELISA - Mouse Apo B ELISA Kit (AB230932)

ELISA

Supplier Data

ELISA - Mouse Apo B ELISA Kit (AB230932)

sELISA

Lab

Sandwich ELISA - Mouse Apo B ELISA Kit (AB230932)

Interpolated concentration of native Apo B was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 2,000 serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Mouse Apo B ELISA Kit (AB230932)

Example of mouse Apo B standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Apo B ELISA Kit (AB230932)

Example of ApoB standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Apo B ELISA Kit (AB230932)

Interpolated concentrations of native mouse ApoB in serum and plasma samples.

The concentrations of ApoB were measured in duplicate, interpolated from the mouse ApoB standard curve and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 2000, plasma (citrate) 1 : 2000, plasma (EDTA) 1 : 2000, and plasma (heparin) 1 : 2000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ApoB concentration was determined to be 187.00 μg/mL in serum, 114.525 μg/mL in plasma (citrate) and 149.723 μg/mL in plasma (EDTA), and 152.208 μg/mL in plasma (Heparin).

sELISA

Supplier Data

Sandwich ELISA - Mouse Apo B ELISA Kit (AB230932)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native mouse ApoB was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Recombinant ApoB was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Mouse Apo B ELISA Kit (AB230932)

Example of ApoB standard curve in Sample Diluent NS. The mouse ApoB standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 196 pg/mL

Range

1.56 - 100 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Apo B ELISA Kit ab230932 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Apo B in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 196 pg/mL.

How the assay works

Mouse Apo B SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Apo B ELISA Kit ab230932 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Mouse Apo B ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Apolipoprotein B antibody [EPR19946-188] - BSA and Azide free (Capture) ab244972
- Anti-Apolipoprotein B antibody [EPR19946-175] - BSA and Azide free (Detector) ab244972

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" } ]

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Recovery

[ { "sample": "Serum", "range": "75 - 95 %", "average": "= 83" }, { "sample": "EDTA Plasma", "range": "79 - 98 %", "average": "= 88" }, { "sample": "Heparin Plasma", "range": "91 - 107 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "75 - 99 %", "average": "= 86" }, { "sample": "Cell culture media", "range": "99 - 107 %", "average": "= 104" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein B often referred to as ApoB is a core protein component of low-density lipoproteins (LDL) and is essential for their assembly and secretion. ApoB is also known as apolipoproteine B and apolipoprotein B protein. This protein has a large molecular weight approximately 550 kDa. ApoB is primarily expressed in the liver and to some extent in the intestine. Its large size allows it to provide structural integrity to lipoprotein particles playing a role in lipid metabolism.

Biological function summary

ApoB plays a significant role in lipid transport and metabolism. It is a critical component of the lipoprotein particle complex which is responsible for the transport of lipids such as cholesterol and triglycerides through the bloodstream. ApoB helps in the binding of lipoproteins to cell receptors facilitating the uptake of lipids by tissues. Its presence on the surface of lipoproteins makes it important for normal cholesterol homeostasis in the human body.

Pathways

ApoB is integrated within the lipid metabolism pathway. It interacts with proteins such as microsomal triglyceride transfer protein (MTP) during the assembly and secretion of lipoproteins. This pathway is essential for the normal transport of lipids throughout the body and influences processes like absorption of dietary fats. ApoB also plays a role in the pathways related to atherosclerosis where it influences the deposition of cholesterol in arterial walls.

ApoB is well-associated with cardiovascular diseases notably atherosclerosis and familial hypercholesterolemia. In these conditions elevated levels of ApoB correlate to an increased risk of plaque formation in blood vessels. ApoB in conjunction with LDL receptor and other related proteins influences lipid homeostasis and disorders where lipid oxidation or accumulation is present. Monitoring ApoB and its concentration can be vital for assessing cardiovascular disease risk.

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Product protocols

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Target data

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

See full target information Apob

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Methods & clinical development 33:101390 PubMed39897642

2025

Silencing hepatic PCSK9 via novel chimeric AAV8 mitigates the progression of atherosclerosis by inhibiting inflammation in ApoE mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaocui Chen,Minghui Sun,Xiang Ma,Yitong Ma,Bangdang Chen

Journal of lipid research 65:100531 PubMed38490635

2024

A targeted proteomics method for quantifying plasma apolipoprotein kinetics in individual mice using stable isotope labeling.

Applications

Unspecified application

Species

Unspecified reactive species

Baohai Shao,Masami Shimizu-Albergine,Farah Kramer,Jenny E Kanter,Jay W Heinecke,Tomas Vaisar,Bettina Mittendorfer,Bruce W Patterson,Karin E Bornfeldt

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e23522 PubMed38445789

2024

Microsomal triglyceride transfer protein is necessary to maintain lipid homeostasis and retinal function.

Applications

Unspecified application

Species

Unspecified reactive species

Catharina R Grubaugh,Anuradha Dhingra,Binu Prakash,Diego Montenegro,Janet R Sparrow,Lauren L Daniele,Christine A Curcio,Brent A Bell,M Mahmood Hussain,Kathleen Boesze-Battaglia

Nutrients 15: PubMed37242292

2023

A Metabolic Enhancer Protects against Diet-Induced Obesity and Liver Steatosis and Corrects a Pro-Atherogenic Serum Profile in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Khrystyna Platko,Paul F Lebeau,Joshua P Nederveen,Jae Hyun Byun,Melissa E MacDonald,Jacqueline M Bourgeois,Mark A Tarnopolsky,Richard C Austin

Communications biology 6:452 PubMed37095219

2023

A human iPSC-derived hepatocyte screen identifies compounds that inhibit production of Apolipoprotein B.

Applications

Unspecified application

Species

Unspecified reactive species

Jui-Tung Liu,Caren Doueiry,Yu-Lin Jiang,Josef Blaszkiewicz,Mary Paige Lamprecht,James A Heslop,Yuri K Peterson,Juliana Debrito Carten,Paula Traktman,Yang Yuan,Salman R Khetani,Waleed O Twal,Stephen A Duncan

American journal of physiology. Endocrinology and metabolism 324:E461-E475 PubMed37053049

2023

Central androgen action reverses hypothalamic astrogliosis and atherogenic risk factors induced by orchiectomy and high-fat diet feeding in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mauricio D Dorfman,Thomas Monfeuga,Susan J Melhorn,Jenny E Kanter,Jeremy M Frey,Rachael D Fasnacht,Anandhakumar Chandran,Emaad Lala,Inmaculada Velasco,Katya B Rubinow,Thomas H Meek,Ellen A Schur,Karin E Bornfeldt,Joshua P Thaler

Heliyon 9:e13985 PubMed36915476

2023

Translational characterization of the temporal dynamics of metabolic dysfunctions in liver, adipose tissue and the gut during diet-induced NASH development in Ldlr-/-.Leiden mice.

Applications

Unspecified application

Species

Unspecified reactive species

Eveline Gart,Wim van Duyvenvoorde,Jessica M Snabel,Christa de Ruiter,Joline Attema,Martien P M Caspers,Serene Lek,Bertie Joan van Heuven,Arjen G C L Speksnijder,Martin Giera,Aswin Menke,Kanita Salic,Kendra K Bence,Gregory J Tesz,Jaap Keijer,Robert Kleemann,Martine C Morrison

Journal of food biochemistry 46:e14501 PubMed36332134

2022

Anti-adiposity and lipid-lowering effects of schisandrol A in diet-induced obese mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sang Ryong Kim,Hyo Jin Park,Un Ju Jung

eLife 11: PubMed36193893

2022

Hepatic inactivation of murine results in marked reduction in plasma cholesterol.

Applications

Unspecified application

Species

Unspecified reactive species

Vi T Tang,Joseph McCormick,Bolin Xu,Yawei Wang,Huan Fang,Xiao Wang,David Siemieniak,Rami Khoriaty,Brian T Emmer,Xiao-Wei Chen,David Ginsburg

Fluids and barriers of the CNS 19:31 PubMed35505336

2022

A novel chronic dural port platform for continuous collection of cerebrospinal fluid and intrathecal drug delivery in free-moving mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tsuneo Nakajima,Shuko Takeda,Yuki Ito,Akane Oyama,Yoichi Takami,Yasushi Takeya,Koichi Yamamoto,Ken Sugimoto,Hideo Shimizu,Munehisa Shimamura,Hiromi Rakugi,Ryuichi Morishita

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Mouse Apo B ELISA Kit

ELISA - Mouse Apo B ELISA Kit (AB230932)

Sandwich ELISA - Mouse Apo B ELISA Kit (AB230932)

Sandwich ELISA - Mouse Apo B ELISA Kit (AB230932)

Sandwich ELISA - Mouse Apo B ELISA Kit (AB230932)

Sandwich ELISA - Mouse Apo B ELISA Kit (AB230932)

Sandwich ELISA - Mouse Apo B ELISA Kit (AB230932)

Sandwich ELISA - Mouse Apo B ELISA Kit (AB230932)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

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