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AB238260

Mouse APOA1 ELISA Kit

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(9 Publications)

Mouse APOA1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse APOA1 with a sensitivity of 11.22 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, Apoa1

4 Images
ELISA - Mouse APOA1 ELISA Kit (AB238260)
  • ELISA

Supplier Data

ELISA - Mouse APOA1 ELISA Kit (AB238260)
Sandwich ELISA - Mouse APOA1 ELISA Kit (AB238260)
  • sELISA

Unknown

Sandwich ELISA - Mouse APOA1 ELISA Kit (AB238260)

Example of mouse APOA1 standard curve in Sample Diluent NS.

The APOA1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse APOA1 ELISA Kit (AB238260)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse APOA1 ELISA Kit (AB238260)

Interpolated concentrations of native APOA1 in mouse serum and plasma samples.

The concentrations of APOA1 were measured in duplicates, interpolated from the APOA1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 50,000, plasma (citrate) 1 : 25,000, plasma (EDTA) 1 : 25,000, and plasma (heparin) 1 : 50,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean APOA1 concentration was determined to be 81.08 ug/mL in serum, 42.54 ug/mL in plasma (citrate), 70.10 ug/mL in plasma (EDTA), and 86.01 ug/mL in plasma (Heparin).

Sandwich ELISA - Mouse APOA1 ELISA Kit (AB238260)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse APOA1 ELISA Kit (AB238260)

Interpolated concentrations of native APOA1 in mouse liver cell culture supernatant sample. The concentrations of APOA1 were measured in duplicates, interpolated from the APOA1 standard curves and corrected for sample dilution. Undiluted sample was liver supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean APOA1 concentration was determined to be 110.43 ng/mL in liver supernatant.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 11.22 pg/mL

Range

117.19 - 9000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse APOA1 ELISA Kit ab238260 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse APOA1 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 11.22 pg/mL.

How the assay works

Mouse APOA1 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse APOA1 ELISA Kit ab238260 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "81 - 101 %", "average": "= 91" }, { "sample": "Serum", "range": "79 - 84 %", "average": "= 82" }, { "sample": "EDTA Plasma", "range": "85 - 90 %", "average": "= 88" }, { "sample": "Heparin Plasma", "range": "88 - 96 %", "average": "= 92" }, { "sample": "Citrate plasma", "range": "91 - 101 %", "average": "= 99" }, { "sample": "Tissue Extracts", "range": "83 - 93 %", "average": "= 90" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab238260-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB238260-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB238260-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB238260-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB238260-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB238260-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BC", "number":"AB238260-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse APOA1 Capture Antibody", "number":"AB238260-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse APOA1 Lyophilized Recombinant Protein", "number":"AB238260-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse APOA1 Detector Antibody", "number":"AB238260-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB238260-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein A-I (ApoA-I) is a major component of high-density lipoprotein (HDL) particles often referred to as 'good cholesterol'. It has a molecular mass of approximately 28 kDa. ApoA-I is mainly expressed in the liver and intestine. It plays a mechanical role in the reverse transport of cholesterol acting to facilitate the efflux of cholesterol from tissues to the liver for excretion. Its alternative names include ApoAI ApoA1 and a component of the AI kits used for measuring this protein.
Biological function summary

ApoA-I functions in cholesterol homeostasis and inflammation. It is a structural component of the HDL complex that mobilizes cholesterol. ApoA-I acts as an activator of the enzyme lecithin-cholesterol acyltransferase (LCAT) which is essential for the maturation of HDL particles. This maturation is necessary for effective cholesterol transport and clearance. ApoA-I's ability to stabilize HDL particles and enhance their functionality makes it significant for maintaining lipid balance and cellular homeostasis.

Pathways

The interaction of ApoA-I with HDL formation and function marks its role in lipid metabolism pathways. Its participation in the reverse cholesterol transport pathway highlights its influence on cardiovascular health. ApoA-I also interacts with other proteins like ApoA-II and paraoxonase-1 which further influence lipid metabolism and antioxidant activities. Understanding these relationships helps elucidate the dynamics of cholesterol removal from the bloodstream.

Disturbances in ApoA-I levels correlate with cardiovascular disease and atherosclerosis. Deficiency or dysfunction in ApoA-I can impair HDL function leading to poor cholesterol removal and buildup within arteries. It is also connected to amyloidosis where misfolded ApoA-I forms deposits in tissues. Understanding these pathological conditions helps researchers target ApoA-I in therapeutic strategies to mitigate disease progression often studying it alongside proteins like ApoB which is associated with low-density lipoprotein (LDL) particles.
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Product protocols

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Target data

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
See full target information Apoa1
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 135: PubMed40371638

2025

Asparagine endopeptidase cleaves apolipoprotein A1 and accelerates pathogenesis of atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengmeng Wang,Bowei Li,Shuke Nie,Xin Meng,Guangxing Wang,Menghan Yang,Wenxin Dang,Kangning He,Tucheng Sun,Ping Xu,Xifei Yang,Keqiang Ye

Experimental & molecular medicine 57:349-363 PubMed39900792

2025

Golgi condensation causes intestinal lipid accumulation through HIF-1α-mediated GM130 ubiquitination by NEDD4.

Applications

Unspecified application

Species

Unspecified reactive species

Hyunsoo Kim,Channy Park,Xiaofan Wei,Arun Chhetri,Laxman Manandhar,Gyuho Jang,Jaetaek Hwang,Batchingis Chinbold,Chagtsalmaa Chuluunbaatar,Hyug Moo Kwon,Raekil Park

Nutrients 15: PubMed37242292

2023

A Metabolic Enhancer Protects against Diet-Induced Obesity and Liver Steatosis and Corrects a Pro-Atherogenic Serum Profile in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Khrystyna Platko,Paul F Lebeau,Joshua P Nederveen,Jae Hyun Byun,Melissa E MacDonald,Jacqueline M Bourgeois,Mark A Tarnopolsky,Richard C Austin

Journal of food biochemistry 46:e14501 PubMed36332134

2022

Anti-adiposity and lipid-lowering effects of schisandrol A in diet-induced obese mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sang Ryong Kim,Hyo Jin Park,Un Ju Jung

International journal of molecular sciences 23: PubMed36293147

2022

Effect of Obesity and High-Density Lipoprotein Concentration on the Pathological Characteristics of Alzheimer's Disease in High-Fat Diet-Fed Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Moonseok Choi,Dongsoo Kim,Young-Jin Youn,Junghwa Ryu,Yun Ha Jeong

Life (Basel, Switzerland) 12: PubMed35207485

2022

Attenuates Atherosclerosis by Regulating Cholesterol Metabolism and Inducing M2 Macrophage Polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Young Song,Haneul Cho,Sora Lee,Kyung Hye Lee,Weon Kim

Circulation 143:921-934 PubMed33228395

2020

Inhibition of Cholesteryl Ester Transfer Protein Preserves High-Density Lipoprotein Cholesterol and Improves Survival in Sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Mark Trinder,Yanan Wang,Christian M Madsen,Tatjana Ponomarev,Lubos Bohunek,Brendan A Daisely,HyeJin Julia Kong,Lisanne L Blauw,Børge G Nordestgaard,Anne Tybjærg-Hansen,Mark M Wurfel,James A Russell,Keith R Walley,Patrick C N Rensen,John H Boyd,Liam R Brunham

JCI insight 4: PubMed31391339

2019

The hepatokine Tsukushi is released in response to NAFLD and impacts cholesterol homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Mathilde Mouchiroud,Étienne Camiré,Manal Aldow,Alexandre Caron,Éric Jubinville,Laurie Turcotte,Inès Kaci,Marie-Josée Beaulieu,Christian Roy,Sébastien M Labbé,Thibault V Varin,Yves Gélinas,Jennifer Lamothe,Jocelyn Trottier,Patricia L Mitchell,Frédéric Guénard,William T Festuccia,Philippe Joubert,Christopher F Rose,Constantine J Karvellas,Olivier Barbier,Mathieu C Morissette,André Marette,Mathieu Laplante

FASEB journal : official publication of the Federa 33:8406-8422 PubMed30964709

2019

4-Phenylbutyrate protects against atherosclerotic lesion growth by increasing the expression of HSP25 in macrophages and in the circulation of mice.

Applications

Unspecified application

Species

Unspecified reactive species

Edward G Lynn,Šárka Lhoták,Paul Lebeau,Jae Hyun Byun,Jack Chen,Khrystyna Platko,Chunhua Shi,Edward R O'Brien,Richard C Austin
View all publications
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