JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB215086

Mouse Apolipoprotein E ELISA Kit

4

(1 Review)

|

(18 Publications)

Mouse Apolipoprotein E ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Apolipoprotein E with a sensitivity of 33.9 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

View Alternative Names

Apolipoprotein E, Apo-E, Apoe

3 Images
ELISA - Mouse Apolipoprotein E ELISA Kit (AB215086)
  • ELISA

Supplier Data

ELISA - Mouse Apolipoprotein E ELISA Kit (AB215086)
Sandwich ELISA - Mouse Apolipoprotein E ELISA Kit (AB215086)
  • sELISA

Lab

Sandwich ELISA - Mouse Apolipoprotein E ELISA Kit (AB215086)

Interpolated concentrations of native Apolipoprotein E in mouse serum and plasma samples.

The concentrations of Apolipoprotein E were measured in duplicates, interpolated from the Apolipoprotein E standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 56,000, plasma (citrate) 1 : 56,000, plasma (EDTA) 1 : 7,000 and plasma (heparin) 1 : 56,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein E concentration was determined to be 155.61 μg/mL in neat serum, 135.57 μg/mL in neat plasma (citrate), 132.0 μg/mL in neat plasma (EDTA), and 161.3 μg/mL in neat plasma (heparin).

Sandwich ELISA - Mouse Apolipoprotein E ELISA Kit (AB215086)
  • sELISA

Lab

Sandwich ELISA - Mouse Apolipoprotein E ELISA Kit (AB215086)

Example of mouse Apolipoprotein E standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 33.9 pg/mL

Range

156.3 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Mouse Apolipoprotein E ELISA Kit ab215086 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Apolipoprotein E in citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 33.9 pg/mL.

How the assay works

Mouse Apolipoprotein E SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Apolipoprotein E ELISA Kit ab215086 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Apolipoprotein E antibody [EPR19665-56] - BSA and Azide free (Capture) ab242844
- Anti-Apolipoprotein E antibody [EPR19665-152] - BSA and Azide free (Detector) ab242844

Apolipoprotein E is a glycoprotein synthesized mainly in the liver and the brain and is a component of most lipoproteins with the exception of low-density lipoproteins (LDL). Apolipoprotein E mediates the binding, internalization, and catabolism of lipoprotein particles. Additionally, it can serve as a ligand for the LDL (Apolipoprotein B/E) receptor and for the specific Apolipoprotein E receptor (chylomicron remnant) of hepatic tissues. Mouse Apolipoprotein E consists of an 18 amino acid (aa) signal peptide and a 293 aa mature chain. Human and rat Apolipoprotein E are 71% and 92% identical to mouse Apolipoprotein E, respectively.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.5" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "97 - 115 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "80 - 102 %", "average": "= 89" }, { "sample": "Heparin Plasma", "range": "91 - 109 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "97 - 103 %", "average": "= 101" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab215086-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB215086-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB215086-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB215086-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB215086-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB215086-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB215086-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Apolipoprotein E Lyophilized Recombinant Protein", "number":"AB215086-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Apolipoprotein E Capture Antibody", "number":"AB215086-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB215086-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Apolipoprotein E Detector Antibody", "number":"AB215086-CMP08", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein E (ApoE) also known as apolipoprotein e or apoE is a major protein involved in lipid metabolism. It has an approximate molecular weight of 34 kDa. This protein is mainly produced in the liver and brain where it plays a critical role in transporting lipoproteins fat-soluble vitamins and cholesterol. ApoE exists in three common isoforms: ApoE2 ApoE3 and ApoE4 each having different impacts on lipid binding and metabolic processes. Scientists often use an ApoE ELISA kit to quantify this protein in various samples providing insights into its expression in health and disease.
Biological function summary

ApoE mediates the binding internalization and catabolism of these lipoprotein particles facilitating their interaction with specific cell-surface receptors such as the LDL receptor. This protein operates as part of a complex that includes various other apolipoproteins and lipid molecules. The study of mouse apoe using tools like a mouse apoe ELISA provides valuable data due to its similar physiological functions in lipid transport and metabolism.

Pathways

In the lipid metabolism pathway ApoE interacts with proteins such as the LDL receptor influencing the clearance of chylomicron remnants and VLDL from the bloodstream. In the cardiovascular disease pathway this protein impacts cholesterol levels and promotes plaques stabilization. ApoE's role in these pathways offers insights into its interaction with related proteins like apolipoprotein B and LDL receptor which are critical for maintaining lipid equilibrium.

In Alzheimer's disease ApoE4 isoform has a higher risk factor compared to ApoE3 and ApoE2 contributing to amyloid plaque formation through interactions with amyloid precursor protein. In cardiovascular diseases ApoE abnormalities influence atherosclerosis development with ApoE-deficient models showing increased susceptibility. ApoE's links to these diseases also connect it to other key proteins such as presenilin-1 in Alzheimer's disease and apolipoprotein B in cardiovascular disorders highlighting its extensive biological impact.
style
left-column

Product protocols

style
left-column

Target data

APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apolipoproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles (By similarity). Finally, APOE has also a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells (PubMed : 23676495).
See full target information Apoe
style
left-column

Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:23989 PubMed39402264

2024

Low to moderate ethanol exposure reduces astrocyte-induced neuroinflammatory signaling and cognitive decline in presymptomatic APP/PS1 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shinwoo Kang,Jeyeon Lee,Dina N Ali,Sun Choi,Jarred Nesbitt,Paul H Min,Eugenia Trushina,Doo-Sup Choi

Alzheimer's & dementia : the journal of the Alzheimer's Association 20:7805-7818 PubMed39345217

2024

Deletion of miR-33, a regulator of the ABCA1-APOE pathway, ameliorates neuropathological phenotypes in APP/PS1 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mason Tate,H R Sagara Wijeratne,Byungwook Kim,Stéphanie Philtjens,Yanwen You,Do-Hun Lee,Daniela A Gutierrez,Daniel Sharify,Megan Wells,Magdalena Perez-Cardelo,Emma H Doud,Carlos Fernandez-Hernando,Cristian Lasagna-Reeves,Amber L Mosley,Jungsu Kim

iScience 27:110006 PubMed38868202

2024

Microglial apolipoprotein E particles contribute to neuronal senescence and synaptotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Na Wang,Lujian Cai,Xinyu Pei,Zhihao Lin,Lihong Huang,Chensi Liang,Min Wei,Lin Shao,Tiantian Guo,Fang Huang,Hong Luo,Honghua Zheng,Xiao-Fen Chen,Lige Leng,Yun-Wu Zhang,Xin Wang,Jie Zhang,Kai Guo,Zhanxiang Wang,Hongsheng Zhang,Yingjun Zhao,Huaxi Xu

EMBO reports 25:2348-2374 PubMed38589666

2024

Lifelong absence of microglia alters hippocampal glutamatergic networks but not synapse and spine density.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Surala,Luna Soso-Zdravkovic,David Munro,Ali Rifat,Koliane Ouk,Imre Vida,Josef Priller,Christian Madry

Pharmaceuticals (Basel, Switzerland) 16: PubMed37375785

2023

Intracellular Drug Delivery Process of Am80-Encapsulated Lipid Nanoparticles Aiming for Alveolar Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Tomomi Akita,Kazuaki Oda,Satoru Narukawa,Yuki Morita,Kota Tange,Yuta Nakai,Chikamasa Yamashita

Journal of neurochemistry 166:346-366 PubMed37303123

2023

Proteomics of the astrocyte secretome reveals changes in their response to soluble oligomeric Aβ.

Applications

Unspecified application

Species

Unspecified reactive species

Vittoria Matafora,Alena Gorb,Fangjia Yang,Wendy Noble,Angela Bachi,Beatriz Gomez Perez-Nievas,Maria Jimenez-Sanchez

STAR protocols 3:101664 PubMed36097382

2022

Protocol to isolate and analyze mouse bone marrow derived dendritic cells (BMDC).

Applications

Unspecified application

Species

Unspecified reactive species

Manuela Sauter,Reinhard J Sauter,Henry Nording,Marcus Olbrich,Frederic Emschermann,Harald F Langer

Frontiers in neuroscience 16:949298 PubMed35844229

2022

Liuwei Dihuang Decoction Alleviates Cognitive Dysfunction in Mice With D-Galactose-Induced Aging by Regulating Lipid Metabolism and Oxidative Stress the Microbiota-Gut-Brain Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Baiyan Liu,Bowei Chen,Jian Yi,Hongping Long,Huiqiao Wen,Fengming Tian,Yingfei Liu,Lan Xiao,Lisong Li

Fluids and barriers of the CNS 19:31 PubMed35505336

2022

A novel chronic dural port platform for continuous collection of cerebrospinal fluid and intrathecal drug delivery in free-moving mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tsuneo Nakajima,Shuko Takeda,Yuki Ito,Akane Oyama,Yoichi Takami,Yasushi Takeya,Koichi Yamamoto,Ken Sugimoto,Hideo Shimizu,Munehisa Shimamura,Hiromi Rakugi,Ryuichi Morishita

Molecular psychiatry 27:3034-3046 PubMed35422468

2022

C/EBPβ/AEP signaling couples atherosclerosis to the pathogenesis of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jianming Liao,Guiqin Chen,Xia Liu,Zheng Zachory Wei,Shan Ping Yu,Qianxue Chen,Keqiang Ye
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB238260

Mouse APOA1 ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse APOA1 ELISA Kit (AB238260)

  • 0
  • 0
Assay Kits

AB190806

Human Apolipoprotein B ELISA Kit

Recombinant|SimpleStep

ELISA - Human Apolipoprotein B ELISA Kit (AB190806)

  • 4
  • 1
Assay Kits

AB263884

Mouse C3 ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse C3 ELISA Kit (AB263884)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com