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AB326431

Mouse Apolipoprotein E ELISA Kit, Chemiluminescent

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Mouse Apolipoprotein E ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse Apolipoprotein E with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 30.48 - 200000 pg/ml with a sensitivity of 20.07pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

Apolipoprotein E, Apo-E, Apoe

4 Images
Sandwich ELISA - Mouse Apolipoprotein E ELISA Kit, Chemiluminescent (AB326431)
  • sELISA

Lab

Sandwich ELISA - Mouse Apolipoprotein E ELISA Kit, Chemiluminescent (AB326431)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Mouse Apolipoprotein E ELISA Kit, Chemiluminescent (AB326431)
  • sELISA

Lab

Sandwich ELISA - Mouse Apolipoprotein E ELISA Kit, Chemiluminescent (AB326431)

Standard curve comparison between the Chemiluminescent Mouse Apolipoprotein E SimpleStep ELISA and colorimetric Mouse Apolipoprotein E SimpleStep ELISA (ab215086) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Apolipoprotein E ELISA Kit, Chemiluminescent (AB326431)
  • sELISA

Lab

Sandwich ELISA - Mouse Apolipoprotein E ELISA Kit, Chemiluminescent (AB326431)

Dynamic range comparison between the Chemiluminescent Mouse Apolipoprotein E SimpleStep ELISA, colorimetric Mouse Apolipoprotein E SimpleStep ELISA (ab215086), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and dynamic range.

Sandwich ELISA - Mouse Apolipoprotein E ELISA Kit, Chemiluminescent (AB326431)
  • sELISA

Lab

Sandwich ELISA - Mouse Apolipoprotein E ELISA Kit, Chemiluminescent (AB326431)

Interpolated concentrations of native Apolipoprotein E in mouse serum and plasma samples.

The concentrations of Apolipoprotein E were measured in duplicates interpolated from the Apolipoprotein E standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 56000 plasma (citrate) 1 : 56000 plasma (EDTA) 1 : 7000 and plasma (heparin) 1 : 56000. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Apolipoprotein E concentration was determined to be 155.61 µg/mL in neat serum 135.57 µg/mL in neat plasma (citrate) 132.0 µg/mL in neat plasma (EDTA) and 161.3 µg/mL in neat plasma (heparin).

Key facts

Detection method

Luminescent

Sample types

Serum, EDTA Plasma, Heparin Plasma, Citrate plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 20.07 pg/mL

Range

30.48 - 200000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Apolipoprotein E ELISA Kit, Chemiluminescent (ab326431) is a rapid single-wash sandwich 55-min ELISA to measure Mouse Apolipoprotein E in Serum, EDTA Plasma, Heparin Plasma, Citrate plasma with a sensitivity of 20.07 pg/mL.

How the assay works

Mouse Apolipoprotein E ELISA Kit, Chemiluminescent (ab326431) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Apolipoprotein E (ab326431) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Apolipoprotein E mouse antibody [EPR19665-56] - BSA and Aside free (Capture) ab242598
- Anti-Apolipoprotein E mouse antibody [EPR19665-152] - BSA and Aside free (Detector) ab242844
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9" } ]
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Recovery

[ { "sample": "Serum", "range": "97 - 115 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "80 - 102 %", "average": "= 89" }, { "sample": "Heparin Plasma", "range": "91 - 109 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "97 - 103 %", "average": "= 101" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326431-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB326431-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB326431-CMP05", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326431-CMP08", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326431-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326431-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326431-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Apolipoprotein E Lyophilized Recombinant Protein", "number":"AB326431-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB326431-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Apolipoprotein E Capture Antibody", "number":"AB326431-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Apolipoprotein E Detector Antibody", "number":"AB326431-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apolipoproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles (By similarity). Finally, APOE also has a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells (PubMed : 23676495).
See full target information Apoe
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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