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AB269541

Mouse Arginase 1 ELISA Kit

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(15 Publications)

Mouse Arginase 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Arginase 1 with a sensitivity of 2.02 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

View Alternative Names

Arginase-1, Liver-type arginase, Type I arginase, Arg1

4 Images
ELISA - Mouse Arginase 1 ELISA Kit (AB269541)
  • ELISA

Supplier Data

ELISA - Mouse Arginase 1 ELISA Kit (AB269541)
Sandwich ELISA - Mouse Arginase 1 ELISA Kit (AB269541)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Arginase 1 ELISA Kit (AB269541)

Interpolated concentrations of native Arginase1 in mouse liver homogenate extract samples based on a 0.25 μg/mL extract load.

The concentrations of Arginase1 were measured in duplicate and interpolated from the Arginase1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Arginase1 concentration was determined to be 636.63 pg/mL in 0.25 μg/mL liver homogenate extract.

Sandwich ELISA - Mouse Arginase 1 ELISA Kit (AB269541)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Arginase 1 ELISA Kit (AB269541)

Example of mouse Arginase1 standard curve in Sample Diluent NS.

The Arginase1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Arginase 1 ELISA Kit (AB269541)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Arginase 1 ELISA Kit (AB269541)

Interpolated concentrations of native Arginase1 in mouse serum and plasma samples.

The concentrations of Arginase1 were measured in duplicates, interpolated from the Arginase1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 40, plasma (citrate) 1 : 40, plasma (heparin) 1 : 20, and plasma (EDTA) 1 : 20. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Arginase1 concentration was determined to be 11.61 ng/mL in serum, 11.86 ng/mL in plasma (citrate), 9.37 ng/mL in plasma (heparin), and 8.70 ng/mL in plasma (EDTA).

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.02 pg/mL

Range

7.81 - 500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Arginase 1 ELISA Kit ab269541 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Arginase 1 in cell lysate, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 2.02 pg/mL.

How the assay works

Mouse Arginase 1 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Arginase 1 ELISA Kit ab269541 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Arginase is a key element of the urea cycle involving the conversion of L-arginine to urea and L-ornithine, which is further metabolized into metabolites that drive collagen synthesis and bioenergetic pathways critical for cell proliferation. Arginine metabolism is a critical regulator of innate and adaptive immune responses, involved in an antimicrobial effector pathway carried out by polymorphonuclear granulocytes (PMN). During PMN apoptosis, arginine is depleted in the microenvironment leading to suppressed T cell and natural killer cell proliferation and cytokine secretion. Arginase also plays a key role in human sexual response in smooth muscle tissue.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" } ]
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Recovery

[ { "sample": "Serum", "range": "83 - 111 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "97 - 104 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "82 - 102 %", "average": "= 95" }, { "sample": "Cell Lysate", "range": "106 - 118 %", "average": "= 111" }, { "sample": "Citrate plasma", "range": "101 - 113 %", "average": "= 106" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (By similarity). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (PubMed : 27043409). Plays a role in the immune response of alternatively activated or M2 macrophages in processes such as wound healing and tissue regeneration, immune defense against multicellular pathogens and parasites, and immune suppression and allergic inflammation; the regulatory outcome seems to be organ specific (PubMed : 19360123, PubMed : 20483789, PubMed : 23552798, PubMed : 23637937, PubMed : 7537672). In tumor-infiltrating dendritic cells (DCs) and myeloid-derived suppressor cells (MDSCs) plays a role in suppression of T cell-mediated antitumor immunity (PubMed : 19414774, PubMed : 23248265).
See full target information Arg1
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 14:e70041 PubMed39868438

2025

Extracellular Vesicles From Bone Marrow-Derived Macrophages Enriched in ARG1 Enhance Microglial Phagocytosis and Haematoma Clearance Following Intracerebral Haemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Libin Hu,Zihang Chen,Jianglong Lu,Shandong Jiang,Haopu Lin,Jiayin Zhou,Ning Wang,Chao Ding,Weifang Ni,Haitao Peng,Yin Li,Xuchao He,Jianru Li,Chaohui Jing,Yang Cao,Hang Zhou,Feng Yan,Gao Chen

Natural products and bioprospecting 14:39 PubMed38954263

2024

5α-Epoxyalantolactone from Inula macrophylla attenuates cognitive deficits in scopolamine-induced Alzheimer's disease mice model.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Ma,Xu-Yao Feng,Jiang-Jiang Tang,Wei Ha,Yan-Ping Shi

Acta biomaterialia 181:222-234 PubMed38648912

2024

In vivo micro-computed tomography evaluation of radiopaque, polymeric device degradation in normal and inflammatory environments.

Applications

Unspecified application

Species

Unspecified reactive species

Kendell M Pawelec,Jeremy M L Hix,Arianna Troia,Keith W MacRenaris,Matti Kiupel,Erik M Shapiro

Journal of natural products 87:639-651 PubMed38477310

2024

Elucidating the Mechanism of Metabolism of Cannabichromene by Human Cytochrome P450s.

Applications

Unspecified application

Species

Unspecified reactive species

Pritam Roy,Jonathan Maturano,Hale Hasdemir,Angel Lopez,Fengyun Xu,Judith Hellman,Emad Tajkhorshid,David Sarlah,Aditi Das

Cancer cell international 23:279 PubMed37980483

2023

Discovery of a new marker to identify myeloid cells associated with metastatic breast tumours.

Applications

Unspecified application

Species

Unspecified reactive species

Ansooya A Bokil,Mathieu Le Boulvais Børkja,Camilla Wolowczyk,Apsana Lamsal,Wenche S Prestvik,Unni Nonstad,Kristine Pettersen,Sonja B Andersen,Anna M Bofin,Geir Bjørkøy,Sjoerd Hak,Miriam S Giambelluca

Frontiers in endocrinology 14:1252727 PubMed37810891

2023

Potential involvement of the bone marrow in experimental Graves' disease and thyroid eye disease.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Gulbins,Mareike Horstmann,Simone Keitsch,Matthias Soddemann,Barbara Wilker,Gregory C Wilson,Ryan Zeidan,Gary D Hammer,Anke Daser,Nikolaos E Bechrakis,Gina-Eva Görtz,Anja Eckstein

Frontiers in immunology 14:1178638 PubMed37388740

2023

Netrin-1 controls inflammation in response to ischemic stroke through altering microglia phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaosheng Yang,Yang Liu,Weijie Zhong,Yi Li,Wenchuan Zhang

Frontiers in immunology 14:1196704 PubMed37215106

2023

Epigenetically altered macrophages promote development of diabetes-associated atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Huang,Wei Gao,Xin Zhong,Hongxian Wu,You Zhou,Yuanji Ma,Juying Qian,Junbo Ge

Frontiers in immunology 14:1170388 PubMed37122744

2023

Nrf2 orchestrates transition from acute to chronic otitis media through inflammatory macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyan Fan,Hongming Xu,Chenling Shen,Jia Fang,Xiaoyan Li

Drug design, development and therapy 17:1125-1138 PubMed37077409

2023

Resveratrol Improves Paclitaxel-Induced Cognitive Impairment in Mice by Activating SIRT1/PGC-1α Pathway to Regulate Neuronal State and Microglia Cell Polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Liu,Miao Tang,Tian-Yi He,Shuang Zhao,Hui-Zhou Li,Zhao Li,Yue-Xian Guo,Xiu-Li Wang
View all publications
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Product promise

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