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AB267590

Mouse ARP1 ELISA Kit

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Mouse ARP1 ELISA Kit is a Sandwich ELISA for the measurement of Mouse ARP1 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Angiopoietin-related protein 1, Angiopoietin-like protein 1, Angptl1

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Sandwich ELISA - Mouse ARP1 ELISA Kit (AB267590)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse ARP1 ELISA Kit (AB267590)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.85 ng/mL

Range

0.819 - 200 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse ARP1 ELISA Kit is designed for the quantitative determination of ARP1 in cell culture supernatants, plasma and serum samples.

Δ Note: Mouse ARP1 concentration is low in normal serum/plasma and may not be detectable in this assay.

This assay employs an antibody specific for mouse ARP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and ARP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse ARP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ARP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "85 - 145 %", "average": "= 127.4" }, { "sample": "Plasma", "range": "73 - 125 %", "average": "= 101.3" }, { "sample": "Cell culture media", "range": "118 - 142 %", "average": "= 132.1" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267590-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB267590-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267590-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267590-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Mouse ARP1 coated Microplate (12 x 8 wells)", "number":"AB267590-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Mouse ARP1 Detection Antibody", "number":"AB267590-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267590-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"900X HRP-Streptavidin Concentrate", "number":"AB267590-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse ARP1 Standard (Lyophilized)", "number":"AB267590-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein ARP1 also known as Actin-Related Protein 1 functions as an important component of the cytoskeleton organization in eukaryotic cells. It is a member of the actin family and shares structural similarities with conventional actin. ARP1 has a mass of approximately 42 kDa. It localizes mostly in the cytoplasm but is also found in the nucleus to some extent. The protein is expressed in a variety of tissues with notable expression in neural tissues.
Biological function summary

ARP1 plays a significant role in cellular processes beyond maintaining structural integrity. It forms part of the dynactin complex which is important for dynein-driven cargo movement along microtubules. This complex includes several other proteins such as p150Glued and dynamitin. By participating in these assemblies ARP1 facilitates vesicular trafficking and is involved in processes like endocytosis and mitosis.

Pathways

ARP1 contributes to vital cellular networks that drive intracellular transport and mitotic spindle orientation. One important pathway where ARP1 acts is the dynein-mediated transport pathway. Within this network ARP1 partners with the protein dynein to facilitate the movement of vesicles and organelles. Another critical pathway is related to mitotic spindle formation where ARP1 influences the positioning and dynamics of the spindle apparatus ensuring accurate chromosome segregation.

ARP1 possesses connections to neurodegenerative diseases and cancer. Alterations in ARP1 function can lead to impaired axonal transport contributing to conditions like Alzheimer's disease. In the context of cancer ARP1 mutations or dysregulation can disrupt normal cellular division and polarity potentially promoting oncogenesis. The association of ARP1 with p150Glued further links it to neurological diseases as mutations in these proteins can exacerbate cellular dysfunction in neurons.
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Product protocols

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Target data

See full target information Angptl1
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websiteProtocolBooklet
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Product promise

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