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AB285300

Mouse Aspartate Aminotransferase ELISA Kit

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Mouse Aspartate Aminotransferase ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse Aspartate Aminotransferase in in Biofluids, Cell/Tissue Extracts samples.

View Alternative Names

Sialin, H(+)/nitrate cotransporter, H(+)/sialic acid cotransporter, Solute carrier family 17 member 5, Vesicular excitatory amino acid transporter, AST, VEAT, Slc17a5

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Sandwich ELISA - Mouse Aspartate Aminotransferase ELISA Kit (AB285300)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Aspartate Aminotransferase ELISA Kit (AB285300)

Standard AST Curve.

Typical Standard Curve : These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Homogenate, Serum

Assay type

Sandwich (quantitative)

Sensitivity

< 0.094 ng/mL

Range

0.156 - 10 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Mouse AST ELISA kit (ab28530, E4320) is a sandwich ELISA assay for the quantitative measurement of AST in Mouse serum, plasma and cell culture supernatants. The density of color is proportional to the amount of Mouse AST captured from the samples.

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.

This product is manufactured by BioVision, an Abcam company and was previously called E4320 Aspartate Aminotransferase (AST) (Mouse) ELISA Kit. E4320-100 is the same size as the 96 test size of ab285300.

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Precision

[ { "reproducibilityType": "Intra", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 8" }, { "reproducibilityType": "Inter", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285300-96Test", "assetComponentDetails": [ { "size":"1 x 2 Vial", "name":"Lyophilized Standard", "number":"AB285300-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample and Standard Dilution Buffer", "number":"AB285300-CMP07", "productcode":"" }, { "size":"1 x 120 µL", "name":"HRP-Streptavidin Conjugate (SABC)", "number":"AB285300-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"SABC dilution buffer", "number":"AB285300-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB285300-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"Antibody dilution buffer", "number":"AB285300-CMP01", "productcode":"" }, { "size":"1 x 120 µL", "name":"Biotin/Detection antibody (Concentrated)", "number":"AB285300-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"25X Wash buffer", "number":"AB285300-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Substrate", "number":"AB285300-CMP09", "productcode":"" }, { "size":"1 x 5 Unit", "name":"Plate sealers", "number":"AB285300-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB285300-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aspartate Aminotransferase (AST) also known as aspartate transaminase or aspartate transferase is an enzyme important for amino acid metabolism. Mechanically it catalyzes the reversible transfer of an amino group from aspartate to α-ketoglutarate forming oxaloacetate and glutamate. AST has a molecular mass of approximately 92 kDa and is expressed in various tissues with high levels found in liver heart muscle and kidneys. Its presence in these tissues highlights its importance in cellular metabolic processes.
Biological function summary

AST facilitates the interconversion between aspartate and oxaloacetate playing a role in the amino acid and urea cycles. Though not part of a large complex AST works closely with similar enzymes such as alanine aminotransferase (ALT) to maintain amino acid balance and support energy production. This enzymatic activity is important in nitrogen metabolism and glutamate use which are necessary for synthesizing other essential molecules within the cell.

Pathways

AST participates in the citric acid cycle and malate-aspartate shuttle enabling efficient energy production and NADH transport. The enzyme assists in converting oxaloacetate a pivotal intermediate in the citric acid cycle to keep the cycle active ensuring efficient cellular respiration. Additionally in the malate-aspartate shuttle AST works alongside malate dehydrogenase to facilitate the transfer of reducing equivalents across the mitochondrial membrane which is essential for ATP generation.

Elevated AST levels often indicate liver damage such as in hepatitis or cirrhosis reflecting its significant tissue expression. The enzyme is also linked to myocardial infarction as damaged heart muscle releases AST into the bloodstream. In these conditions AST serves as a biomarker for tissue damage often in conjunction with ALT levels allowing clinicians to assess the extent of liver or cardiac injury.
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Product protocols

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Target data

Multifunctional anion transporter that operates via two distinct transport mechanisms, namely proton-coupled anion cotransport and membrane potential-dependent anion transport (By similarity) (PubMed : 18695252). Electroneutral proton-coupled acidic monosaccharide symporter, with a sugar to proton stoichiometry of 1 : 1. Exports glucuronic acid and free sialic acid derived from sialoglycoconjugate degradation out of lysosomes, driven by outwardly directed lysosomal pH gradient. May regulate lysosome function and metabolism of sialylated conjugates that impact oligodendrocyte lineage differentiation and myelinogenesis in the central nervous system (By similarity) (PubMed : 20007460). Electrogenic proton-coupled nitrate symporter that transports nitrate ions across the basolateral membrane of salivary gland acinar cells, with nitrate to proton stoichiometry of 2 : 1. May contribute to nitrate clearance from serum by salivary glands, where it is further concentrated and secreted in the saliva (By similarity). Uses membrane potential to drive the uptake of acidic amino acids and peptides into synaptic vesicles. Responsible for synaptic vesicular storage of L-aspartate and L-glutamate in pinealocytes as well as vesicular uptake of N-acetyl-L-aspartyl-L-glutamate neuropeptide, relevant to aspartegic-associated glutamatergic neurotransmission and activation of metabotropic receptors that inhibit subsequent transmitter release (PubMed : 18695252, PubMed : 23889254).. Receptor for CM101, a polysaccharide produced by group B Streptococcus with antipathoangiogenic properties.
See full target information Slc17a5
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