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AB263882

Mouse AST ELISA Kit (Aspartate Aminotransferase)

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(1 Review)

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(51 Publications)

Mouse AST ELISA kit is a single-wash, 90-minute SimpleStep ELISA® assay designed for the quantification of mouse aspartate aminotransferase (AST/cAspAT) with a sensitivity of 39 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric sandwich ELISA with a 450 nm readout; compatible with any standard plate reader
- Available in different formats to meet various throughput needs, including 384-well plates for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready, carrier-dree antibody pair.
- Cited in over 25 publications

View Alternative Names

cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, Got1

8 Images
ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)
  • ELISA

Supplier Data

ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)
Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)

Interpolated concentrations of native AST in mouse serum and plasma samples.

The concentrations of AST were measured in duplicates, interpolated from the AST standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 40, plasma (citrate) 1 : 40, plasma (EDTA) 1 : 40 and plasma (heparin) 1 : 40. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 271 ng/mL in serum, 307 ng/mL in plasma (citrate), 197 ng/mL in plasma (EDTA), and 190 ng/mL in plasma (heparin).

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)

Example of mouse AST standard curve in Sample Diluent NS.

The AST standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)

Interpolated concentrations of native AST in mouse liver extract and NIH3T3 cell extract samples based on a 3,125 ng/mL and 12,500 ng/mL extract load, respectively.

The concentrations of AST were measured in duplicate, interpolated from the AST standard curve, and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 4,352 pg/mL in mouse liver extract and 6,385 pg/mL in NIH3T3 cell extract.

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)
  • sELISA

Lab

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)

Example of mouse AST standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)
  • sELISA

Lab

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)

Interpolated concentration of native AST was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1,560 ng/mL mouse liver extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)
  • sELISA

Lab

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)

Example of mouse AST standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)
  • sELISA

Lab

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)

Interpolated concentration of native AST was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 40 mouse serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 39 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse AST ELISA Kit (Aspartate Aminotransferase) ab263882 is a rapid single-wash 90-min ELISA kit to measure tissue lysate, cell lysate, serum and plasma. This SimpleStep ELISA® sensitivity is 39 pg/mL.

How the assay works
SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity
This kit is designed for the quantification of mouse AST. The standard protein in this kit is full length mouse AST. Native signal was detected in serum, plasma (citrate), plasma (EDTA), plasma (heparin), cell extract, and tissue extract sample types. Cell culture supernatant, urine, saliva, milk, and CSF samples have not been tested with this kit.
Recombinant Human IL-1 beta, Mouse IL-1 alpha, and Mouse IL-1 receptor1 were prepared at 5 ng/mL and assayed for cross reactivity. No cross-reactivity was observed.
Recombinant Human IL-1 beta, Mouse IL-1 alpha, and Mouse IL-1 receptor1 were prepared at 5 ng/mL and tested for interference. No interference with was observed.

Mouse AST ELISA Kit (Aspartate Aminotransferase) ab263882 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Mouse AST ELISA Kit ab263882
Mouse AST ELISA Kit ab263882 has been used to measure AST plasma to study mitochondrial homeostatis in liver disease(1) and rencofilstat as a treatment for HCV-induced hepatocellular carcinoma(2).

References:
(1)Ai-Lei Xu et al. 2024, PMID: 37902450,
(2)Philippe Gallay et al. 2023, PMID: 37896876

Save your precious samples
Our 384-well Mouse AST ELISA Kit (Aspartate Aminotransferase) only requires a maxmum of 12.5µL

Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Mouse/Rat Aspartate Aminotransferase Antibody Pair - BSA and Azide free ab267702
- Anti-Aspartate Aminotransferase antibody [EPR20781-22] - BSA and Azide free (Capture) ab259695
- Anti-Aspartate Aminotransferase antibody [EPR22206-177] - BSA and Azide free (Detector) ab259696

Related and recommended products
Mouse AST ELISA Kit ab263882 is commonly used by researchers to measure liver function.

Other ELISA kits to measure liver function include:
- Mouse ALT ELISA kit ab234578
- Mouse Albumin ELISA kit ab282297
- Mouse Alkaline Phosphatase ELISA kit ab285274

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" } ]
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Recovery

[ { "sample": "Serum", "range": "90 - 105 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "87 - 104 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "94 - 107 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "110 - 118 %", "average": "= 115" }, { "sample": "Cell Lysate", "range": "99 - 102 %", "average": "= 101" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aspartate Aminotransferase (AST) also known as aspartate transaminase or aspartate transferase is an enzyme important for amino acid metabolism. Mechanically it catalyzes the reversible transfer of an amino group from aspartate to α-ketoglutarate forming oxaloacetate and glutamate. AST has a molecular mass of approximately 92 kDa and is expressed in various tissues with high levels found in liver heart muscle and kidneys. Its presence in these tissues highlights its importance in cellular metabolic processes.
Biological function summary

AST facilitates the interconversion between aspartate and oxaloacetate playing a role in the amino acid and urea cycles. Though not part of a large complex AST works closely with similar enzymes such as alanine aminotransferase (ALT) to maintain amino acid balance and support energy production. This enzymatic activity is important in nitrogen metabolism and glutamate use which are necessary for synthesizing other essential molecules within the cell.

Pathways

AST participates in the citric acid cycle and malate-aspartate shuttle enabling efficient energy production and NADH transport. The enzyme assists in converting oxaloacetate a pivotal intermediate in the citric acid cycle to keep the cycle active ensuring efficient cellular respiration. Additionally in the malate-aspartate shuttle AST works alongside malate dehydrogenase to facilitate the transfer of reducing equivalents across the mitochondrial membrane which is essential for ATP generation.

Elevated AST levels often indicate liver damage such as in hepatitis or cirrhosis reflecting its significant tissue expression. The enzyme is also linked to myocardial infarction as damaged heart muscle releases AST into the bloodstream. In these conditions AST serves as a biomarker for tissue damage often in conjunction with ALT levels allowing clinicians to assess the extent of liver or cardiac injury.
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Product protocols

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Target data

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
See full target information Got1
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Publications (51)

Recent publications for all applications. Explore the full list and refine your search

Hepatology communications 9: PubMed40536537

2025

Rbpj deletion in hepatic progenitor cells attenuates endothelial responses and fibrosis in DDC-fed mice.

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Sanghoon Lee,Lu Ren,Weiwei Li,Aditi Paranjpe,Ping Zhou,Andrew Potter,Stacey S Huppert,Soona Shin

RSC advances 15:20143-20167 PubMed40519680

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Exploration of the anticancer efficacy of a novel 1,3-thiazole analog in an ehrlich ascites carcinoma model: and insights into hepatorenal protective potentials the modulation of apoptosis, oxidative stress and inflammation.

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Mohammed El Behery,Dina M Abo-Elmatty,Maha Alsunbul,Yassmina I Mohey El-Deen,Doaa I Mohamed,Emad Rashad Sindi,Maha H Hashem,Ebtesam Al-Olayan,Ibrahim Abdel Aziz Ibrahim,Ghazi A Bamagous,Suraj Mali,Essa M Saied

Chemical biology & drug design 105:e70113 PubMed40317895

2025

Osthole Induces Hepatic Stellate Cell Ferroptosis to Alleviate Liver Fibrosis by Inhibiting the Y-Box Binding Protein 1-Wnt/β-Catenin Axis Through Downregulating Myocyte Enhancer Factor 2A.

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Ming Tong,Meng Liu,Liang Chen,Yi-He Lin,Qing Zheng

Nature communications 16:1806 PubMed39984450

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Targeted delivery of TGF-β mRNA to murine lung parenchyma using one-component ionizable amphiphilic Janus Dendrimers.

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Jaclynn A Meshanni,Emily R Stevenson,Dapeng Zhang,Rachel Sun,Nathan A Ona,Erin K Reagan,Elena Abramova,Chang-Jiang Guo,Melissa Wilkinson,Ishana Baboo,Yuzi Yang,Liuyan Pan,Devendra S Maurya,Virgil Percec,Yongsheng Li,Andrew Gow,Drew Weissman,Elena N Atochina-Vasserman

Life metabolism 3:loae026 PubMed39873005

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Phytic acid-based nanomedicine against mTOR represses lipogenesis and immune response for metabolic dysfunction-associated steatohepatitis therapy.

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Fenghua Xu,Shoujie Zhao,Yejing Zhu,Jun Zhu,Lingyang Kong,Huichen Li,Shouzheng Ma,Bo Wang,Yongquan Qu,Zhimin Tian,Junlong Zhao,Lei Liu

BMJ open diabetes research & care 13: PubMed39842865

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Mechanism of TGIF1 on glycolipid metabolism disorders in mice with type 2 diabetes.

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Fuyan Bai,Liping Zheng,Li Tao,Shikai Wang,Yuchen Li,Lijun Hou

Frontiers in nutrition 11:1469952 PubMed39742098

2025

High-fat diet mouse model receiving L-glucose supplementations propagates liver injury.

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Johnny Amer,Athar Amleh,Ahmad Salhab,Yuval Kolodny,Shira Yochelis,Baker Saffouri,Yossi Paltiel,Rifaat Safadi

Frontiers in pharmacology 15:1484278 PubMed39654627

2024

Osteopontin neutralization increases vitamin D receptors on NKT cells and ameliorates liver fibrosis by promoting their activity.

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Johnny Amer,Ahmad Salhab,Enas Hussini,Rasha Shweiki,Iman Zahran,Mohammad Far

International journal of molecular sciences 25: PubMed39596109

2024

Vine Tea Extract (VTE) Inhibits High-Fat Diet-Induced Adiposity: Evidence of VTE's Anti-Obesity Effects In Vitro and In Vivo.

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Wonchul Lim,Seongmin Choi,Jinhak Kim,Kwang-Soo Baek,Minkuk Park,Gakyung Lee,Tae-Gyu Lim

Nature metabolism 6:2281-2299 PubMed39578649

2024

Pathogenic role of acyl coenzyme A binding protein (ACBP) in Cushing's syndrome.

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Hui Pan,Ai-Ling Tian,Hui Chen,Yifan Xia,Allan Sauvat,Stephanie Moriceau,Flavia Lambertucci,Omar Motiño,Liwei Zhao,Peng Liu,Misha Mao,Sijing Li,Shuai Zhang,Adrien Joseph,Sylvère Durand,Fanny Aprahamian,Zeyu Luo,Yang Ou,Zhe Shen,Enfu Xue,Yuhong Pan,Vincent Carbonnier,Gautier Stoll,Sabrina Forveille,Marion Leduc,Giulia Cerrato,Alexandra Cerone,Maria Chiara Maiuri,Frederic Castinetti,Thierry Brue,Hongsheng Wang,Yuting Ma,Isabelle Martins,Oliver Kepp,Guido Kroemer
View all publications
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