Mouse Axl ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Axl with a sensitivity of 10.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 10.8 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response.
Alternative names
Ark, Ufo, Axl, Tyrosine-protein kinase receptor UFO, Adhesion-related kinase
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Recommended products
Mouse Axl ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Axl with a sensitivity of 10.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 10.8 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 5 | mean - | SD - | C.V. 2.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 3.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 106 | Range 101 - 114 % |
Sample type Serum | Average % = 104 | Range 91 - 116 % |
Sample type EDTA Plasma | Average % = 100 | Range 86 - 111 % |
Sample type Urine | Average % = 109 | Range 104 - 114 % |
Sample type Heparin Plasma | Average % = 83 | Range 74 - 91 % |
Sample type Citrate plasma | Average % = 102 | Range 90 - 111 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse Axl ELISA Kit (ab207619) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Axl protein in cell culture supernatant, serum, urine, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Axl with 10.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Axl is a transmembrane receptor tyrosine kinase on the plasma membrane. The growth factor GAS6 is a ligand for Axl and regulates many physiological processes including cell survival, cell proliferation, migration and differentiation. The antibodies used in this kit were raised against a portion of the extracellular domain of Axl.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Axl protein also known as AXL receptor tyrosine kinase plays a significant role in cell signaling. It has a molecular weight of approximately 98 kDa. Axl is a transmembrane receptor that is expressed in a variety of tissues including the immune system reproductive organs and the central nervous system. It is mainly recognized for transmitting signals from the extracellular matrix into the cytoplasm by binding with its ligand Gas6.
- Biological function summary
Axl is important in mediating cell survival proliferation and migration. It functions as part of the TAM family of receptors which also includes Tyro3 and Mer. Axl often forms complexes with these receptors to facilitate efficient signaling. This interaction triggers phosphorylation events that activate downstream signaling proteins reinforcing its influence in cellular functions.
- Pathways
Axl is deeply involved in the PI3K-Akt and MAPK signaling pathways which are central to cellular growth and survival. In these pathways Axl interacts closely with proteins like PI3K and Akt to regulate processes such as apoptosis and metabolism. Its interaction with these pathways places Axl as an important modulator of intracellular signaling cascades.
- Associated diseases and disorders
Axl has been implicated in cancer progression and immune response dysregulation. In many cancer types Axl overexpression links to tumor invasion metastasis and resistance to therapy. Moreover Axl’s role in autoimmune diseases becomes evident through its association with the Gas6 protein. Exploring these connections furthers understanding of how Axl's aberrant activity influences health and disease.
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3 product images
Sandwich ELISA - Mouse Axl ELISA Kit (ab207619)
Example of mouse Axl standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Axl ELISA Kit (ab207619)
Interpolated concentrations of native Axl in mouse serum, plasma, and urine samples.
The concentrations of Axl were measured in duplicates, interpolated from the Axl standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 4%, plasma (citrate) 4%, plasma (EDTA) 4%, and urine 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Axl concentration was determined to be 113 ng/mL in serum, 73.3 ng/mL in plasma (citrate), 88.9 ng/mL in plasma (heparin), and 112 ng/mL in urine.
Sandwich ELISA - Mouse Axl ELISA Kit (ab207619)
Comparison of secreted Axl in unstimulated and PMA/PHA-stimulated L929 Cells.
L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. Axl was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the Axl Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 169 pg/mL unstimulated, 696 pg/mL stimulated, and undetectable in media.
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