Mouse BAFF ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse BAFF with a sensitivity of 7.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 7.4 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Isoform 2 seems to inhibit isoform 1 secretion and bioactivity.
Alternative names
CD257, Baff, Tnfsf13b, Tumor necrosis factor ligand superfamily member 13B, B-cell-activating factor, BAFF
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Mouse BAFF ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse BAFF with a sensitivity of 7.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 7.4 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Mouse Serum | n 8 | mean - | SD - | C.V. 3.3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Mouse Serum | n 3 | mean - | SD - | C.V. 1.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 102 | Range 101 - 103 % |
Sample type EDTA Plasma | Average % = 98 | Range 95 - 102 % |
Sample type Heparin Plasma | Average % = 96 | Range 91 - 101 % |
Sample type Citrate plasma | Average % = 98 | Range 91 - 103 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse BAFF ELISA Kit (ab216948) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BAFF protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse BAFF with 7.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
B-cell activating factor (BAFF) protein is a 309 amino acid cytokine encoded by the TNFSF13B gene. BAFF has been previously referred to as B Lymphocyte Stimulator (BLys), TNF- and APOL- related leukocyte expressed ligand (TALL-1), and Dendritic cell-derived TNF-like molecule (CD257 antigen). BAFF protein is a member of the tumor necrosis factor (TNF) superfamily of ligands. The BAFF cytokine acts as a potent B-cell activator promoting the proliferation and differentiation of B-cells. It is expressed as a type II transmembrane protein on various cell types including monocytes, dendritic cells, and bone marrow stromal cells. The extracellular portion can be cleaved from the membrane, generating a soluble protein fragment. Inadequate levels of BAFF fail to activate B-cells to produce enough immunoglobulin and lead to immunodeficiency. Excessive levels of BAFF cause abnormally high antibody production, resulting in systemic lupus erythematosus, rheumatoid arthritis, and other autoimmune diseases.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
BAFF also known as B-cell activating factor or TNFSF13B is a protein important for B-cell survival and maturation. It's a type II transmembrane protein expressed in cells like macrophages and dendritic cells and has a mass of approximately 31 kDa. The BAFF protein is also present on activated T-cells and in the serum as a soluble form. It functions as a cytokine and influences various stages of B-cell development.
- Biological function summary
BAFF plays a significant role in the humoral immune response by enhancing B-cell proliferation and function. It belongs to the tumor necrosis factor (TNF) ligand family and interacts as a homotrimer. The BAFF cytokine can bind to receptors such as BAFF-R BCMA (B-cell maturation antigen) and TACI (transmembrane activator and CAML interactor). These interactions regulate B-cell homeostasis promoting development and differentiation processes important for normal immune responses.
- Pathways
BAFF is central to the regulation of immune system signaling particularly in B-cell receptor (BCR) signaling and NF-kB pathway activation. The BAFF-R mediates B-cell survival signals through the NF-kB pathway while TACI and BCMA associations convey additional immune responses regulation. These multiple pathways ensure the maintenance of B-cell populations and their ability to produce antibodies.
- Associated diseases and disorders
BAFF has been linked to conditions such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Elevated levels of BAFF can exacerbate autoimmune responses due to increased B-cell survival. Connections with these disorders involve the BAFF-R and its influence on excessive B-cell proliferation contributing to disease pathology. Therapeutically anti-BAFF therapies target these interactions to modify disease progression and manage autoimmunity.
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3 product images
Sandwich ELISA - Mouse BAFF ELISA Kit (ab216948)
Mouse BAFF standard curve comparison data.
Standard curve comparison between mouse BAFF SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
Sandwich ELISA - Mouse BAFF ELISA Kit (ab216948)
Example of mouse BAFF standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse BAFF ELISA Kit (ab216948)
Interpolated concentrations of native BAFF in mouse serum and plasma samples.
The concentrations of BAFF were measured in duplicate, interpolated from the BAFF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (citrate) 5%, plasma (EDTA) 5% and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BAFF concentration was determined to be 22 ng/mL in serum, 16 ng/mL in plasma (citrate), 20 ng/mL in plasma (EDTA), and 24 ng/mL in plasma (heparin).
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