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AB119580

Mouse BAFF ELISA Kit (TNFSF13B)

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(3 Publications)

Mouse BAFF ELISA Kit (TNFSF13B) is a Sandwich ELISA for the measurement of Mouse BAFF (TNFSF13B) in Mouse in Biofluids, Cell Culture Media, Cell/Tissue Extracts samples.

View Alternative Names

CD257, Baff, Tnfsf13b, Tumor necrosis factor ligand superfamily member 13B, B-cell-activating factor, BAFF

1 Images
Sandwich ELISA - Mouse BAFF ELISA Kit (TNFSF13B) (AB119580)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse BAFF ELISA Kit (TNFSF13B) (AB119580)

Representative Standard Curve using ab119580.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum, Cell Lysate

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

46.9 - 3000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's mouse BAFF (TNFSF13B) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse BAFF (TNFSF13B) in cell culture supernatants, serum and plasma (heparin, EDTA).

A BAFF (TNFSF13B) specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for BAFF (TNFSF13B) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse BAFF (TNFSF13B) amount of sample captured in plate.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "82 pg/mL", "standardDeviation": "6.47", "coefficientOfVariability": "= 7.9" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "76 pg/mL", "standardDeviation": "7.14", "coefficientOfVariability": "= 9.4" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "1209 pg/mL", "standardDeviation": "72.54", "coefficientOfVariability": "= 6" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "1221 pg/mL", "standardDeviation": "67.15", "coefficientOfVariability": "= 5.5" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "287 pg/mL", "standardDeviation": "15.52", "coefficientOfVariability": "= 5.3" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "287 pg/mL", "standardDeviation": "16.07", "coefficientOfVariability": "= 5.6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119580-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB119580-CMP05", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti- mouse BAFF antibody", "number":"AB119580-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-mouse BAFF antibody Microplate (12 x 8 wells)", "number":"AB119580-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB119580-CMP07", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB119580-CMP10", "productcode":"" }, { "size":"1 x 4 Unit", "name":"Plate Seal", "number":"AB119580-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB119580-CMP09", "productcode":"" }, { "size":"2 x 10 ng", "name":"Lyophilized recombinant mouse BAFF standard", "number":"AB119580-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB119580-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB119580-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB119580-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BAFF also known as B-cell activating factor or TNFSF13B is a protein important for B-cell survival and maturation. It's a type II transmembrane protein expressed in cells like macrophages and dendritic cells and has a mass of approximately 31 kDa. The BAFF protein is also present on activated T-cells and in the serum as a soluble form. It functions as a cytokine and influences various stages of B-cell development.
Biological function summary

BAFF plays a significant role in the humoral immune response by enhancing B-cell proliferation and function. It belongs to the tumor necrosis factor (TNF) ligand family and interacts as a homotrimer. The BAFF cytokine can bind to receptors such as BAFF-R BCMA (B-cell maturation antigen) and TACI (transmembrane activator and CAML interactor). These interactions regulate B-cell homeostasis promoting development and differentiation processes important for normal immune responses.

Pathways

BAFF is central to the regulation of immune system signaling particularly in B-cell receptor (BCR) signaling and NF-kB pathway activation. The BAFF-R mediates B-cell survival signals through the NF-kB pathway while TACI and BCMA associations convey additional immune responses regulation. These multiple pathways ensure the maintenance of B-cell populations and their ability to produce antibodies.

BAFF has been linked to conditions such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Elevated levels of BAFF can exacerbate autoimmune responses due to increased B-cell survival. Connections with these disorders involve the BAFF-R and its influence on excessive B-cell proliferation contributing to disease pathology. Therapeutically anti-BAFF therapies target these interactions to modify disease progression and manage autoimmunity.
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Product protocols

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Target data

Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.. Isoform 2 seems to inhibit isoform 1 secretion and bioactivity.
See full target information Tnfsf13b
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

iScience 24:103076 PubMed34585117

2021

Targeting B cells in the pre-phase of systemic autoimmunity globally interferes with autoimmune pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Anja Werner,Simon Schäfer,Olga Zaytseva,Heike Albert,Anja Lux,Jasminka Krištić,Marija Pezer,Gordan Lauc,Thomas Winkler,Falk Nimmerjahn

JCI insight 5: PubMed32434989

2020

Effects of B cell-activating factor on tumor immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Mark Yarchoan,Won Jin Ho,Aditya Mohan,Yajas Shah,Teena Vithayathil,James Leatherman,Lauren Dennison,Neeha Zaidi,Sudipto Ganguly,Skylar Woolman,Kayla Cruz,Todd D Armstrong,Elizabeth M Jaffee

Molecular immunology 75:28-37 PubMed27213814

2016

VNAR single-domain antibodies specific for BAFF inhibit B cell development by molecular mimicry.

Applications

Unspecified application

Species

Unspecified reactive species

Julien Häsler,Martin F Flajnik,Gareth Williams,Frank S Walsh,J Lynn Rutkowski
View all publications
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