AB233624

Mouse Bax ELISA Kit

Recombinant
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(5 Publications)

Mouse Bax ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse Bax in Mouse in Cell/Tissue Extracts samples.

View Alternative Names

Apoptosis regulator BAX, Bax

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Sandwich ELISA - Mouse Bax ELISA Kit (AB233624)

sELISA

Supplier Data

Sandwich ELISA - Mouse Bax ELISA Kit (AB233624)

Example of mouse Bax standard curve in 1X Cell Extraction Buffer PTR.

The Bax standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Bax ELISA Kit (AB233624)

Comparison of Bax distribution in subcellular fractions derived from 3.7x103 HeLa cells and whole cells cultured in the presence (treated) or absence (untreated) of 1 µM staurosporine for 4 hours.

Cells were collected directly after treatment and subcellular fractions were prepared using a cell fractionation kit (ab109719). The concentrations of Bax were measured in three different dilutions of the fraction samples in duplicate and interpolated from the Bax standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean Bax concentration was determined to be 841.4 pg/mL in the untreated cytosol fraction, 100.0 pg/mL in the untreated mitochondrial fraction, 912.6 pg/mL in the untreated whole cell sample, 186.9 pg/mL in the treated cytosol fraction, 1,366 pg/mL in the treated mitochondrial fraction, and 1,622 pg/mL in the treated whole cell sample.

sELISA

Supplier Data

Sandwich ELISA - Mouse Bax ELISA Kit (AB233624)

Interpolated concentrations of native Bax in mouse cell extracts.

Interpolated concentrations of native Bax in mouse C2C12 cell extract based on a 12.5 μg/mL extract load, A20 cell extract based on a 5 μg/mL extract load, and PC-12 cell extract based on a 25 μg/mL extract load. The concentrations of Bax were measured in duplicate and interpolated from the Bax standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Bax concentration was determined to be 3,001 pg/mL in C2C12 cell extract, 1,404 pg/mL in A20 cell extract, and 1,689 pg/mL in PC-12 cell extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse Bax ELISA Kit (AB233624)

Other species reactivity was determined by measuring Bax in rat and human cell extracts within the working range of the assay, interpolating the protein concentrations from the mouse standard curve.

The mean Bax concentration was determined to be 243.5 ng/mg in C2C12 cell extract, 65.15 ng/mg in PC-12 cell extract, and 39.85 ng/mg in HeLa cell extract.

Key facts

Detection method

Colorimetric

Sample types

Tissue Homogenate, Cell Lysate

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 19.4 pg/mL

Range

46.9 - 3000 pg/mL

Assay time

2h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Bax ELISA Kit (ab233624) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Bax protein in tissue homogenate and cell lysate. Quantitate Mouse Bax with 19.4 pg/ml sensitivity.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Cell Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.3" }, { "reproducibilityType": "Intra", "sample": "Cell Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.1" } ]

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Recovery

[ { "sample": "Cell culture extracts", "range": "97 - 108 %", "average": "= 101" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab233624-1x96Tests", "assetComponentDetails": [ { "size":"2 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB233624-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB233624-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB233624-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB233624-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB233624-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB233624-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Bax Capture Antibody", "number":"AB233624-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB233624-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Bax Lyophilized Recombinant Protein", "number":"AB233624-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB233624-CMP05", "productcode":"" }, { "size":"2 x 100 µL", "name":"Streptavidin-HRP", "number":"AB233624-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse 10X Detector Antibody", "number":"AB233624-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB233624-CMP11", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bax also known as Bcl-2-associated X protein plays an important role in the regulation of apoptosis. It is widely known for its pro-apoptotic function opposing the action of anti-apoptotic proteins like Bcl-2. Bax has a molecular weight of approximately 21 kDa making it identifiable in laboratory techniques such as western blot. Researchers often use anti-Bax antibodies to detect and analyze its expression levels in various studies. The Bax protein is mainly expressed in mitochondria where it undergoes changes in response to apoptotic signals.

Biological function summary

The Bax protein forms homodimers or heterodimers with other Bcl-2 family proteins to facilitate apoptosis. This protein is part of a larger complex involving several members of the Bcl-2 family which finely tunes the balance between cell survival and programmed cell death. Through a change in its conformation Bax translocates to the outer mitochondrial membrane where it promotes the release of cytochrome c and other apoptogenic factors into the cytosol.

Pathways

Bax plays a significant role in the intrinsic pathway of apoptosis also known as the mitochondrial pathway. This pathway involves the release of cytochrome c which further activates downstream proteins such as caspase-9 and caspase-3. Bax interacts closely with proteins like Bak another pro-apoptotic member of the Bcl-2 family providing a cooperative function in mitochondrial membrane permeabilization. Together Bax and Bak act as gatekeepers of the mitochondrial pathway determining the cell's fate in response to apoptotic stimuli.

The dysregulation of Bax often associates with various cancers and neurodegenerative diseases. In cancer reduced Bax expression or function can lead to resistance to apoptosis contributing to unchecked cellular proliferation. Conversely in neurodegenerative disorders such as Alzheimer's disease increased Bax activity results in excessive neuronal apoptosis. The balance between Bax and its partner proteins like Bcl-2 or Bcl-xL is important in maintaining normal cellular function and preventing disease progression.

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Product protocols

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Target data

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. BAX deficiency leads to lymphoid hyperplasia and male sterility, because of the cessation of sperm production.

See full target information Bax

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed39405093

2024

A new potential strategy for cutaneous squamous cell carcinoma treatment by generating serum-based antibodies from tumor-exposed mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Liu

International journal of biological sciences 19:4657-4671 PubMed37781037

2023

Pgam5-mediated PHB2 dephosphorylation contributes to endotoxemia-induced myocardial dysfunction by inhibiting mitophagy and the mitochondrial unfolded protein response.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Cai,Ziying Li,Zemao Zheng,Zhongzhou Guo,Qian Li,Shuxian Deng,Nengxian Shi,Qing Ou,Hao Zhou,Zhigang Guo,Zhongqing Chen,Hang Zhu

Journal of advanced research 41:39-48 PubMed36328752

2022

DNA-PKcs promotes sepsis-induced multiple organ failure by triggering mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Rongjun Zou,Jun Tao,Junxiong Qiu,Huimin Lu,Jianhua Wu,Hang Zhu,Ruibing Li,David Mui,Sam Toan,Xing Chang,Hao Zhou,Xiaoping Fan

Journal of cellular and molecular medicine 25:10028-10038 PubMed34612609

2021

A comparative analysis of deferoxamine treatment modalities for dermal radiation-induced fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher V Lavin,Darren B Abbas,Evan J Fahy,Daniel K Lee,Michelle Griffin,Nestor M Diaz Deleon,Shamik Mascharak,Kellen Chen,Arash Momeni,Geoffrey C Gurtner,Michael T Longaker,Derrick C Wan

Frontiers in pharmacology 10:1156 PubMed31636565

2019

A Single δ-Tetrahydrocannabinol (THC) Dose During Brain Development Affects Markers of Neurotrophy, Oxidative Stress, and Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Gaëtan Philippot,Erica Forsberg,Caroline Tahan,Henrik Viberg,Robert Fredriksson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse Bax ELISA Kit

Sandwich ELISA - Mouse Bax ELISA Kit (AB233624)

Sandwich ELISA - Mouse Bax ELISA Kit (AB233624)

Sandwich ELISA - Mouse Bax ELISA Kit (AB233624)

Sandwich ELISA - Mouse Bax ELISA Kit (AB233624)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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