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AB223590

Mouse Beta-2-Microglobulin ELISA Kit

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(3 Publications)

Mouse Beta-2-Microglobulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Beta-2-Microglobulin with a sensitivity of 1.165 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Beta-2-microglobulin, B2m

6 Images
ELISA - Mouse Beta-2-Microglobulin ELISA Kit (AB223590)
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Supplier Data

ELISA - Mouse Beta-2-Microglobulin ELISA Kit (AB223590)
Sandwich ELISA - Mouse Beta-2-Microglobulin ELISA Kit (AB223590)
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Supplier Data

Sandwich ELISA - Mouse Beta-2-Microglobulin ELISA Kit (AB223590)

Interpolated concentrations of native Beta-2-Microglobulin in mouse serum, and plasma samples.

The concentrations of Beta-2-Microglobulin were measured in duplicates, interpolated from the Beta-2-Microglobulin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 100,000, plasma (citrate) 1 : 100,000, plasma (heparin) 1 : 100,000, and plasma (EDTA) 1 : 100,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Beta-2-Microglobulin concentration was determined to be 19.48 μg/mL in neat serum, 15.15 μg/mL in neat plasma (citrate), 18.74 μg/mL in neat plasma (heparin), and 15.87 μg/mL in neat plasma (EDTA).

Sandwich ELISA - Mouse Beta-2-Microglobulin ELISA Kit (AB223590)
  • sELISA

Lab

Sandwich ELISA - Mouse Beta-2-Microglobulin ELISA Kit (AB223590)

Example of mouse Beta-2-Microglobulin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Beta-2-Microglobulin ELISA Kit (AB223590)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Beta-2-Microglobulin ELISA Kit (AB223590)

Interpolated concentrations of native Beta-2-Microglobulin in mouse urine and lung tissue culture supernatant samples.

The concentrations of Beta-2-Microglobulin were measured in duplicates, interpolated from the Beta-2-Microglobulin standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1 : 100, lung supernatant 1 : 5,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Beta-2-Microglobulin concentration was determined to be 16.12 ng/mL in neat urine, 440.7 ng/mL in neat lung tissue culture supernatant.

Sandwich ELISA - Mouse Beta-2-Microglobulin ELISA Kit (AB223590)
  • sELISA

Lab

Sandwich ELISA - Mouse Beta-2-Microglobulin ELISA Kit (AB223590)

Interpolated concentration of native Beta-2-Microglobulin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 100,000 mouse serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

Sandwich ELISA - Mouse Beta-2-Microglobulin ELISA Kit (AB223590)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Beta-2-Microglobulin ELISA Kit (AB223590)

Example of mouse Beta-2-Microglobulin standard curve in Sample Diluent NS + Enhancer.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 1.165 pg/mL

Range

3.125 - 200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Beta-2-Microglobulin ELISA Kit ab223590 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Beta-2-Microglobulin in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, urine. This SimpleStep sensitivity is 1.165 pg/mL.

How the assay works

Mouse Beta-2-Microglobulin SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Beta-2-Microglobulin ELISA Kit ab223590 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Mouse Beta-2-Microglobulin ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-beta 2 Microglobulin antibody [EPR20530-260] - BSA and Azide free (Capture) ab244922
- Anti-beta 2 Microglobulin antibody [EPR20530-203] - BSA and Azide free (Detector) ab244922

Beta-2-Microglobulin is the light chain of the class I major histocompatibility complex (MHC); it forms heterodimers with classical (HLA-A, -B, -C) and non-classical (HLA-E, -F, -G) class I MHC heavy chains and with class I non-MHC heavy chains (CD1). Beta-2-Microglobulin is involved in the presentation of peptide antigens to the immune system. Beta-2-Microglobulin can dissociate from the MHC and circulate in complex with alpha 2-Macroglobulin.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "110 - 118 %", "average": "= 114" }, { "sample": "Serum", "range": "97 - 106 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "101 - 107 %", "average": "= 103" }, { "sample": "Urine", "range": "95 - 100 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "82 - 103 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "101 - 103 %", "average": "= 102" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC I) and plays an important role in presenting peptides to the immune system. B2M weighs approximately 11.8 kDa and is found abundantly in all nucleated cells. It has alternate names such as B2 microglobulin or beta-2-microglobulin. This protein is present in the cell membrane as a part of the MHC I which is important for immune surveillance. Additionally B2M is detectable in various biological fluids including serum and its levels can reflect physiological and pathological states.
Biological function summary

Beta-2-microglobulin is important for the stability and transport of MHC class I molecules to the cell surface. As part of the MHC class I complex B2M assists in binding peptides allowing immune cells to identify and target pathogen-infected cells. Without B2M the MHC class I molecules are not properly expressed on the cell surface disrupting immune recognition. In laboratory settings researchers often use anti-beta-2-microglobulin antibodies to investigate its role in MHC class I function.

Pathways

Beta-2-microglobulin interacts significantly with the immune system most notably in the antigen processing and presentation pathway. It works alongside proteins such as the heavy chain of MHC class I. B2M is important in the pathway that involves the transport of antigens to the endoplasmic reticulum where they are loaded onto MHC class I molecules for inspection by cytotoxic T cells. Another related pathway is the tapasin-mediated processing of antigen peptides highlighting the indispensable role of B2M in immune response regulation.

Beta-2-microglobulin is associated with conditions such as beta-2-microglobulin amyloidosis and certain lymphoproliferative disorders. Elevated levels of B2M in serum serve as a marker for diseases like multiple myeloma where the protein level correlates with disease severity. B2M-related amyloidosis frequently occurs in patients undergoing long-term dialysis where amyloid deposits accumulate in tissues. Linking B2M to immune system dysfunction studies have shown interactions with other proteins including components of the immune system like HLA-A and HLA-B highlighting B2M's relevance in diagnosing and understanding these conditions.
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Product protocols

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Target data

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
See full target information B2m
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 14: PubMed40298637

2025

and Flower Extract Complex Alleviates Kidney Inflammation and Fibrosis by Modulating Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Soyoung Kim,Jeongwon Kim,Jong-Lae Kim,Mi-Ryeong Park,Kye Won Park,Ki Wung Chung

Nature communications 14:1223 PubMed36869030

2023

Polynucleotide phosphorylase protects against renal tubular injury via blocking mt-dsRNA-PKR-eIF2α axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yujie Zhu,Mingchao Zhang,Weiran Wang,Shuang Qu,Minghui Liu,Weiwei Rong,Wenwen Yang,Hongwei Liang,Caihong Zeng,Xiaodong Zhu,Limin Li,Zhihong Liu,Ke Zen

Bioengineered 12:11188-11200 PubMed34784849

2021

Isoliquiritigenin attenuates acute renal injury through suppressing oxidative stress, fibrosis and JAK2/STAT3 pathway in streptozotocin-induced diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Leiming Sun,Zheng Yang,Jiaying Zhang,Jie Wang
View all publications
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