Mouse beta IG H3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse beta IG H3 with a sensitivity of 40 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.16 - 10 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 40 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Plays a role in cell adhesion (PubMed:8024701). May play a role in cell-collagen interactions (By similarity).
Alternative names
Transforming growth factor-beta-induced protein ig-h3, Beta ig-h3, Tgfbi
What's included?
Recommended products
Mouse beta IG H3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse beta IG H3 with a sensitivity of 40 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.16 - 10 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 40 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample EDTA Plasma | n 8 | mean - | SD - | C.V. 4.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample EDTA Plasma | n 3 | mean - | SD - | C.V. 4.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type EDTA Plasma | Average % = 95 | Range 90 - 102 % |
Sample type Heparin Plasma | Average % = 107 | Range 105 - 110 % |
Sample type Citrate plasma | Average % = 89 | Range 83 - 93 % |
Sample type Cell culture media | Average % = 107 | Range 103 - 109 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse beta IG H3 ELISA Kit (ab206987) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of beta IG H3 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse beta IG H3 with 40 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Mouse beta IG H3 (TGFBI) is a 683 amino acid (aa) protein with a 23 aa signal peptide, one EMI domain, four FAS1 domains, and one RGD motif. Mouse beta IG H3 is expressed as a 75 kDa protein with no post-translational additions. Following secretion, cleavages at multiple positions near the C-terminal end release peptides with pro-apoptotic activity. The four FAS1 domains of Mouse beta IG H3 contain YH motifs, which allow Mouse beta IG H3 to bind to matrix fibronectin, collagen I, collagen VI, biglycan, and decorin. Mouse beta IG H3 is an adaptor protein that is induced in most cell types in response to TGF-beta stimulation. It is upregulated in keratinocytes and immature dendritic cells but downregulated in osteoblasts. Mouse beta IG H3 shares 91% aa sequence identity with human and porcine beta IG H3.
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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6 product images
Sandwich ELISA - Mouse beta IG H3 ELISA Kit (ab206987)
Example of the mouse beta IG H3 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse beta IG H3 ELISA Kit (ab206987)
Example of the mouse beta IG H3 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse beta IG H3 ELISA Kit (ab206987)
Linearity of dilution of mouse beta IG H3 in plasma samples.
Native mouse beta IG H3 was measured in 0.8% mouse plasma citrate, 0.8% mouse plasma EDTA, and 0.33% mouse plasma heparin samples diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse beta IG H3 were measured in duplicate and interpolated from the mouse beta IG H3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
Sandwich ELISA - Mouse beta IG H3 ELISA Kit (ab206987)
Linearity of dilution of mouse beta IG H3 in cell culture media.
Recombinant mouse beta IG H3 was spiked into 50% cell culture media without FBS and into 10% cell culture media with FBS and diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse beta IG H3 were measured in duplicate and interpolated from the mouse beta IG H3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
Sandwich ELISA - Mouse beta IG H3 ELISA Kit (ab206987)
Linearity of mouse beta IG H3 in mouse eyeball tissue extract.
Native mouse beta IG H3 was measured in 100 μg/mL of mouse eyeball extract diluted in a 2-fold dilution series in Sample Diluent 1X Cell Extraction Buffer PTR. The concentrations of mouse beta IG H3 were measured in duplicate and interpolated from the mouse beta IG H3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
Sandwich ELISA - Mouse beta IG H3 ELISA Kit (ab206987)
Assay specificity is demonstrated on 3T3-L1 cells.
3T3-L1 cells were cultured in cell culture media in the presence and absence 1 μM Dexamethasone, 0.5 mM IBMX, 1 μg/mL insulin for 10 days to generate adipocytes and undifferentiated cells respectively. At the end of the treatment, protein extraction was carried out according to section 11.4 of the booklet. Both 3T3-L1 adipocytes and undifferentiated cell extracts were measured in duplicate at 1 mg/mL using this kit. The concentrations of beta IG H3 were measured in duplicate and interpolated from the beta IG H3 standard. The interpolated dilution factor corrected values are graphed (mean +/- SD).
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