AB206987

Mouse beta IG H3 ELISA Kit

Recombinant
SimpleStep
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Mouse beta IG H3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse beta IG H3 with a sensitivity of 40 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Transforming growth factor-beta-induced protein ig-h3, Beta ig-h3, Tgfbi

6 Images

Sandwich ELISA - Mouse beta IG H3 ELISA Kit (AB206987)

sELISA

Supplier Data

Sandwich ELISA - Mouse beta IG H3 ELISA Kit (AB206987)

Assay specificity is demonstrated on 3T3-L1 cells.

3T3-L1 cells were cultured in cell culture media in the presence and absence 1 μM Dexamethasone, 0.5 mM IBMX, 1 μg/mL insulin for 10 days to generate adipocytes and undifferentiated cells respectively. At the end of the treatment, protein extraction was carried out according to section 11.4 of the booklet. Both 3T3-L1 adipocytes and undifferentiated cell extracts were measured in duplicate at 1 mg/mL using this kit. The concentrations of beta IG H3 were measured in duplicate and interpolated from the beta IG H3 standard. The interpolated dilution factor corrected values are graphed (mean +/- SD).

sELISA

Supplier Data

Sandwich ELISA - Mouse beta IG H3 ELISA Kit (AB206987)

Linearity of dilution of mouse beta IG H3 in plasma samples.

Native mouse beta IG H3 was measured in 0.8% mouse plasma citrate, 0.8% mouse plasma EDTA, and 0.33% mouse plasma heparin samples diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse beta IG H3 were measured in duplicate and interpolated from the mouse beta IG H3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).

sELISA

Supplier Data

Sandwich ELISA - Mouse beta IG H3 ELISA Kit (AB206987)

Example of the mouse beta IG H3 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse beta IG H3 ELISA Kit (AB206987)

Linearity of mouse beta IG H3 in mouse eyeball tissue extract.

Native mouse beta IG H3 was measured in 100 μg/mL of mouse eyeball extract diluted in a 2-fold dilution series in Sample Diluent 1X Cell Extraction Buffer PTR. The concentrations of mouse beta IG H3 were measured in duplicate and interpolated from the mouse beta IG H3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).

sELISA

Supplier Data

Sandwich ELISA - Mouse beta IG H3 ELISA Kit (AB206987)

Example of the mouse beta IG H3 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse beta IG H3 ELISA Kit (AB206987)

Linearity of dilution of mouse beta IG H3 in cell culture media.

Recombinant mouse beta IG H3 was spiked into 50% cell culture media without FBS and into 10% cell culture media with FBS and diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse beta IG H3 were measured in duplicate and interpolated from the mouse beta IG H3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 40 pg/mL

Range

0.16 - 10 ng/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse beta IG H3 ELISA Kit (ab206987) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of beta IG H3 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse beta IG H3 with 40 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Mouse beta IG H3 (TGFBI) is a 683 amino acid (aa) protein with a 23 aa signal peptide, one EMI domain, four FAS1 domains, and one RGD motif. Mouse beta IG H3 is expressed as a 75 kDa protein with no post-translational additions. Following secretion, cleavages at multiple positions near the C-terminal end release peptides with pro-apoptotic activity. The four FAS1 domains of Mouse beta IG H3 contain YH motifs, which allow Mouse beta IG H3 to bind to matrix fibronectin, collagen I, collagen VI, biglycan, and decorin. Mouse beta IG H3 is an adaptor protein that is induced in most cell types in response to TGF-beta stimulation. It is upregulated in keratinocytes and immature dendritic cells but downregulated in osteoblasts. Mouse beta IG H3 shares 91% aa sequence identity with human and porcine beta IG H3.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "EDTA Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" }, { "reproducibilityType": "Intra", "sample": "EDTA Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" } ]

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Recovery

[ { "sample": "EDTA Plasma", "range": "90 - 102 %", "average": "= 95" }, { "sample": "Heparin Plasma", "range": "105 - 110 %", "average": "= 107" }, { "sample": "Citrate plasma", "range": "83 - 93 %", "average": "= 89" }, { "sample": "Cell culture media", "range": "103 - 109 %", "average": "= 107" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab206987-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB206987-CMP13", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB206987-CMP12", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB206987-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse beta IG H3 Lyophilized Recombinant Protein", "number":"AB206987-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Mouse beta IG H3 Capture Antibody (lyophilized)", "number":"AB206987-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB206987-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB206987-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB206987-CMP07", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB206987-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB206987-CMP11", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Mouse beta IG H3 Detector Antibody (lyophilized)", "number":"AB206987-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB206987-CMP06", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Plays a role in cell adhesion (PubMed : 8024701). May play a role in cell-collagen interactions (By similarity).

See full target information Tgfbi

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse beta IG H3 ELISA Kit

Sandwich ELISA - Mouse beta IG H3 ELISA Kit (AB206987)

Sandwich ELISA - Mouse beta IG H3 ELISA Kit (AB206987)

Sandwich ELISA - Mouse beta IG H3 ELISA Kit (AB206987)

Sandwich ELISA - Mouse beta IG H3 ELISA Kit (AB206987)

Sandwich ELISA - Mouse beta IG H3 ELISA Kit (AB206987)

Sandwich ELISA - Mouse beta IG H3 ELISA Kit (AB206987)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Complementary Products

Reagents, Controls & Accessories

Assay Kits

Primary Antibodies

Secondary Antibodies

Product promise